Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HU754_RS28375 Genome accession   NZ_CP077090
Coordinates   6435560..6436594 (-) Length   344 a.a.
NCBI ID   WP_116254322.1    Uniprot ID   A0A9E6NP64
Organism   Pseudomonas zeae strain OE 48.2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6430560..6441594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU754_RS28345 (HU754_028345) ruvX 6430687..6431124 (+) 438 WP_064589634.1 Holliday junction resolvase RuvX -
  HU754_RS28350 (HU754_028350) pyrR 6431205..6431711 (+) 507 WP_034153653.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  HU754_RS28355 (HU754_028355) - 6431739..6432743 (+) 1005 WP_007913665.1 aspartate carbamoyltransferase catalytic subunit -
  HU754_RS28360 (HU754_028360) - 6432740..6434011 (+) 1272 WP_099757416.1 dihydroorotase -
  HU754_RS28365 (HU754_028365) - 6434105..6434536 (-) 432 WP_007913651.1 TM2 domain-containing protein -
  HU754_RS28370 (HU754_028370) - 6434785..6435408 (+) 624 WP_093429838.1 C40 family peptidase -
  HU754_RS28375 (HU754_028375) pilT 6435560..6436594 (-) 1035 WP_116254322.1 type IV pilus twitching motility protein PilT Machinery gene
  HU754_RS28380 (HU754_028380) - 6436651..6437337 (+) 687 WP_186620030.1 YggS family pyridoxal phosphate-dependent enzyme -
  HU754_RS28385 (HU754_028385) proC 6437361..6438179 (+) 819 WP_186620031.1 pyrroline-5-carboxylate reductase -
  HU754_RS28390 (HU754_028390) - 6438190..6438780 (+) 591 WP_099757419.1 YggT family protein -
  HU754_RS28395 (HU754_028395) - 6438777..6439073 (+) 297 WP_186620032.1 DUF167 domain-containing protein -
  HU754_RS28400 (HU754_028400) - 6439301..6440440 (+) 1140 WP_186620033.1 homoserine O-acetyltransferase -
  HU754_RS28405 (HU754_028405) metW 6440448..6441068 (+) 621 WP_034153662.1 methionine biosynthesis protein MetW -
  HU754_RS28410 (HU754_028410) - 6441095..6441529 (+) 435 WP_186620035.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38069.76 Da        Isoelectric Point: 7.0158

>NTDB_id=579066 HU754_RS28375 WP_116254322.1 6435560..6436594(-) (pilT) [Pseudomonas zeae strain OE 48.2]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGALGMQTLDMCL
KELVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=579066 HU754_RS28375 WP_116254322.1 6435560..6436594(-) (pilT) [Pseudomonas zeae strain OE 48.2]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGAGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAACTGATCTACGACA
TCATGAACGATACCCAGCGGGTCGACTTCGAGAAGCATCTGGAAACCGACTTTTCCTTCGAAGTGCCGGGTGTCGCGCGT
TTCCGCGTCAACGCTTTCAACCAGAACCGTGGCGCAGGCGCCGTATTCCGTACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTTGCCATGGGCGACGTCTTCCGCAAGATCACCGATGCCCCGCGCGGTCTGGTGTTGGTCACCGGGCCGACCGGTT
CCGGCAAATCGACCACGCTGGCGGCGATGATCGATTACCTCAATACCCACCGGCATCACCACATCCTGACTATCGAAGAT
CCGATCGAGTTTGTTCACGAATCGCGCAAATGCCTGATCAATCAGCGTGAAGTCCACCGTGATACCCGCAGTTTCGCCAC
TGCGTTGCGCTCGGCACTGCGTGAGGACCCGGACGTGATTCTGGTCGGCGAGATGCGTGACCTGGAAACCATTCGCCTGG
CGCTGACCGCAGCCGAAACCGGGCACTTGGTATTCGGCACGCTGCACACCACCTCGGCGGCGAAAACCATCGACCGCGTG
GTCGACGTGTTCCCCGGTGACGAGAAGTCGATGGTGCGCTCGATGCTGTCGGAGTCGTTGCTGGCGGTGGTCTCGCAGAC
GCTGATCAAGAAGATCGGCGGCGGGCGGGTGGCGGCGCACGAGATCATGTTGGGGACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATTCAGACGGGTGGGGCGTTGGGCATGCAGACACTGGACATGTGCCTG
AAGGAACTGGTGACCAAGGGCTTGATCAGTCGCGAGCATGCGCGGGAGAAGGCGCGCACGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

73.077

98.256

0.718
  pilT Legionella pneumophila strain ERS1305867

73.077

98.256

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398