Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HU759_RS28750 Genome accession   NZ_CP077082
Coordinates   6365908..6366942 (-) Length   344 a.a.
NCBI ID   WP_186616996.1    Uniprot ID   -
Organism   Pseudomonas sp. OE 28.3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6360908..6371942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU759_RS28720 (HU759_028720) ruvX 6360995..6361432 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  HU759_RS28725 (HU759_028725) pyrR 6361515..6362021 (+) 507 WP_017139446.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  HU759_RS28730 (HU759_028730) - 6362046..6363050 (+) 1005 WP_056857699.1 aspartate carbamoyltransferase catalytic subunit -
  HU759_RS28735 (HU759_028735) - 6363047..6364318 (+) 1272 WP_099550338.1 dihydroorotase -
  HU759_RS28740 (HU759_028740) - 6364464..6364892 (-) 429 WP_186616998.1 TM2 domain-containing protein -
  HU759_RS28745 (HU759_028745) - 6365142..6365777 (+) 636 WP_017139450.1 C40 family peptidase -
  HU759_RS28750 (HU759_028750) pilT 6365908..6366942 (-) 1035 WP_186616996.1 type IV pilus twitching motility protein PilT Machinery gene
  HU759_RS28755 (HU759_028755) - 6367000..6367686 (+) 687 WP_099550335.1 YggS family pyridoxal phosphate-dependent enzyme -
  HU759_RS28760 (HU759_028760) proC 6367720..6368538 (+) 819 WP_169987580.1 pyrroline-5-carboxylate reductase -
  HU759_RS28765 (HU759_028765) - 6368564..6369151 (+) 588 WP_017139454.1 YggT family protein -
  HU759_RS28770 (HU759_028770) - 6369346..6370485 (+) 1140 WP_138450794.1 homoserine O-acetyltransferase -
  HU759_RS28775 (HU759_028775) metW 6370493..6371113 (+) 621 WP_016972957.1 methionine biosynthesis protein MetW -
  HU759_RS28780 (HU759_028780) - 6371134..6371568 (+) 435 WP_138813460.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37328.20 Da        Isoelectric Point: 7.2989

>NTDB_id=578876 HU759_RS28750 WP_186616996.1 6365908..6366942(-) (pilT) [Pseudomonas sp. OE 28.3]
MDITELLTASVRRGASDLHLSAGLAPMLRVDGEIWPLEGPVLSPTQVADLLSPLLNQYQQKDFETSLETDFAFALPGLAR
FRANVFRQDRGMGAVFRTIPTEVQSLEDLGLGEVFRRIAQLPRGLVLVTGATGSGKSTTLAAMIDHLNQHRRQHILTLED
PIEFIHRPKMALINQRQVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAAKSVDRL
VDVFPAGEKAMVRAMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVSAGLISREDARGLARVPTDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=578876 HU759_RS28750 WP_186616996.1 6365908..6366942(-) (pilT) [Pseudomonas sp. OE 28.3]
ATGGATATCACTGAACTGCTGACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCATTTATCGGCTGGCCTGGCGCCAAT
GCTGCGTGTCGATGGCGAAATCTGGCCATTGGAGGGGCCGGTGCTTTCGCCTACGCAAGTTGCGGACTTATTAAGCCCTT
TGCTCAACCAGTACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCGTTCGCGCTGCCGGGCCTGGCGCGG
TTCCGCGCAAATGTGTTCCGCCAGGATCGCGGCATGGGCGCAGTGTTTCGCACTATCCCGACCGAAGTGCAAAGCCTGGA
AGACCTCGGATTAGGCGAGGTCTTCCGCCGTATCGCGCAGTTGCCGCGCGGCCTGGTACTGGTGACTGGGGCAACCGGCT
CCGGCAAGTCGACGACCCTGGCGGCGATGATTGACCACCTCAACCAGCACCGGCGCCAGCACATTCTCACGCTGGAAGAC
CCGATCGAATTTATCCACAGGCCGAAAATGGCCCTGATCAACCAGCGCCAGGTACACCGCGACACTCATAGTTTTTCGGC
GGCGCTGCGCTCGGCCCTGCGTGAAGACCCGGATGTGATTCTGGTGGGCGAGCTGCGTGACCTGGAAACCATCCGCCTGG
CACTGACGGCGGCAGAGACCGGGCACTTGGTATTGGGCACGCTGCACACCACTTCAGCGGCAAAGAGCGTGGATCGGCTG
GTGGATGTGTTCCCTGCGGGGGAAAAAGCCATGGTGCGCGCCATGCTGTCGGAGTCGCTGCAGGCGGTGGTGTCCCAGGT
GCTGGTTAAGAAAATCGGCGGCGGCCGGGTGGCGGCGCACGAGATCATGCTGGGCACGCCAGCTATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGGTCTCGGCGATTCAAACCGGCGGGGCGTTGGGGATGAAAACGCTGGACATGAGCTTG
AAGGCGTTGGTCAGCGCAGGGCTGATCAGCCGGGAGGATGCGCGGGGGCTGGCGCGGGTGCCGACAGATATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.512

100

0.715
  pilT Pseudomonas stutzeri DSM 10701

69.767

100

0.698
  pilT Acinetobacter nosocomialis M2

69.208

99.128

0.686
  pilT Acinetobacter baumannii D1279779

68.915

99.128

0.683
  pilT Acinetobacter baumannii strain A118

68.915

99.128

0.683
  pilT Acinetobacter baylyi ADP1

68.622

99.128

0.68
  pilT Legionella pneumophila strain Lp02

63.314

98.256

0.622
  pilT Legionella pneumophila strain ERS1305867

63.314

98.256

0.622
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.202

97.674

0.608
  pilT Vibrio cholerae strain A1552

62.202

97.674

0.608
  pilT Neisseria meningitidis 8013

60.714

97.674

0.593
  pilT Neisseria gonorrhoeae MS11

60.417

97.674

0.59
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.176

98.837

0.506
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398