Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KR767_RS02970 Genome accession   NZ_CP077072
Coordinates   654783..655892 (+) Length   369 a.a.
NCBI ID   WP_284732868.1    Uniprot ID   -
Organism   Luteibacter anthropi strain SM7.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 620712..660289 654783..655892 within 0


Gene organization within MGE regions


Location: 620712..660289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KR767_RS02840 (KR767_02850) - 620712..623519 (+) 2808 WP_250878019.1 glycosyltransferase family 1 protein -
  KR767_RS02845 (KR767_02855) - 623651..624397 (+) 747 WP_250878020.1 electron transfer flavoprotein subunit beta/FixA family protein -
  KR767_RS02850 (KR767_02860) - 624422..625363 (+) 942 WP_250878021.1 FAD-binding protein -
  KR767_RS02855 (KR767_02865) - 625496..626896 (+) 1401 WP_250879213.1 UbiA family prenyltransferase -
  KR767_RS02860 (KR767_02870) - 626896..628197 (+) 1302 WP_250878022.1 FAD-binding oxidoreductase -
  KR767_RS02865 (KR767_02875) - 628201..628938 (+) 738 WP_250878023.1 SDR family oxidoreductase -
  KR767_RS02870 (KR767_02880) - 628945..629352 (+) 408 WP_250878024.1 GtrA family protein -
  KR767_RS02875 (KR767_02885) - 629418..631694 (+) 2277 WP_250878025.1 hypothetical protein -
  KR767_RS02880 (KR767_02890) - 631955..633304 (-) 1350 WP_284732867.1 phosphomannomutase -
  KR767_RS02885 (KR767_02895) - 633337..634242 (-) 906 WP_250878027.1 NAD-dependent epimerase/dehydratase family protein -
  KR767_RS02890 (KR767_02900) gmd 634220..635254 (-) 1035 WP_250878028.1 GDP-mannose 4,6-dehydratase -
  KR767_RS02895 (KR767_02905) - 635296..639066 (-) 3771 WP_250878029.1 glycosyltransferase -
  KR767_RS02900 (KR767_02910) - 639063..640967 (-) 1905 WP_250878030.1 FkbM family methyltransferase -
  KR767_RS02905 (KR767_02915) - 640964..642190 (-) 1227 WP_250878031.1 ABC transporter ATP-binding protein -
  KR767_RS02910 (KR767_02920) - 642187..642876 (-) 690 WP_250878032.1 ABC transporter permease -
  KR767_RS02915 (KR767_02925) - 643051..644211 (-) 1161 WP_250878033.1 PLP-dependent aspartate aminotransferase family protein -
  KR767_RS02920 (KR767_02930) - 644583..646001 (+) 1419 WP_284732886.1 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase -
  KR767_RS02925 (KR767_02935) rfbB 646097..647140 (+) 1044 WP_250878035.1 dTDP-glucose 4,6-dehydratase -
  KR767_RS02930 (KR767_02940) rfbA 647137..648024 (+) 888 WP_250878036.1 glucose-1-phosphate thymidylyltransferase RfbA -
  KR767_RS02935 (KR767_02945) rfbC 648021..648575 (+) 555 WP_250878037.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  KR767_RS02940 (KR767_02950) rfbD 648575..649495 (+) 921 WP_250878038.1 dTDP-4-dehydrorhamnose reductase -
  KR767_RS02945 (KR767_02955) glmS 649507..651336 (-) 1830 WP_250878039.1 glutamine--fructose-6-phosphate transaminase (isomerizing) -
  KR767_RS02950 (KR767_02960) - 651351..652721 (-) 1371 WP_250878040.1 pyridoxal-phosphate dependent enzyme -
  KR767_RS02955 (KR767_02965) - 652938..653171 (-) 234 WP_345779891.1 YdcH family protein -
  KR767_RS02960 (KR767_02970) - 653499..654161 (-) 663 WP_166951623.1 DUF4398 domain-containing protein -
  KR767_RS02965 (KR767_02975) - 654163..654687 (-) 525 WP_166951620.1 DUF4398 domain-containing protein -
  KR767_RS02970 (KR767_02980) pilU 654783..655892 (+) 1110 WP_284732868.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KR767_RS02975 (KR767_02985) maiA 656081..656725 (-) 645 WP_166951615.1 maleylacetoacetate isomerase -
  KR767_RS02980 (KR767_02990) - 656921..658102 (+) 1182 WP_250878041.1 phosphoglycerate kinase -
  KR767_RS02985 (KR767_02995) - 658102..658734 (+) 633 WP_250878042.1 HAD hydrolase-like protein -
  KR767_RS02990 (KR767_03000) - 659435..660289 (+) 855 WP_250878043.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41014.36 Da        Isoelectric Point: 6.5080

>NTDB_id=578618 KR767_RS02970 WP_284732868.1 654783..655892(+) (pilU) [Luteibacter anthropi strain SM7.4]
MDIGYFLKLMVDKGASDMFLTTGAPVNIKVEGKLYPLGNTGLPAGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEIGMVIRAIKSEIPSLEELRLPAIFRELVMEPRGLILVVGATGSGKSTTLAAMIDHRNQNTPGHILTIED
PIEYLHRHKRSIVNQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDTETMEAAIAFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVIGKDGRRVPAVEVLLNTPLIRDMIRRGQIHEVKDAMDRSLQEGMQTFDQSL
YRLYKDGQITLDECLAKADSRDGLALKIRLSEGAPGNEFASSDPYGIGV

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=578618 KR767_RS02970 WP_284732868.1 654783..655892(+) (pilU) [Luteibacter anthropi strain SM7.4]
GTGGATATTGGTTATTTCCTCAAGCTAATGGTGGACAAGGGCGCGTCGGACATGTTCCTGACGACCGGTGCCCCGGTGAA
CATCAAGGTGGAGGGCAAGCTTTACCCGCTCGGCAATACGGGCCTGCCGGCGGGAATGGTCAAGAAGATCGCCTACTCCC
TGATGGACGAAGGCCAGGTGCCCCAGTTCGAGCGCGACCTGGAACTGAACATGGCCCTGGCCGTGAAGGAAGCCGGACGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAGATCGGCATGGTGATCCGTGCCATCAAGAGCGAGATCCCCTCGCTTGA
GGAACTGCGCCTGCCGGCGATCTTCCGTGAGCTGGTGATGGAGCCGCGCGGGCTGATTCTGGTGGTGGGCGCCACGGGGT
CGGGCAAGTCCACCACCCTGGCCGCGATGATCGACCACCGCAACCAGAACACCCCGGGACACATCCTCACCATCGAGGAC
CCCATCGAATACCTGCACCGGCACAAGCGTTCCATCGTGAACCAGCGCGAGGTGGGGCTGGATACCCACAGCTATCACGA
GGCCCTCAAGAACGCGATGCGTGAGGCGCCGGACGTGATCATGATCGGCGAGATCCGCGACACGGAGACCATGGAGGCGG
CCATTGCCTTCTCCGAGACGGGCCACCTGTGCCTGGCCACCCTGCACTCGAACAATGCCGACCAGACCCTGGAACGCATC
CTCAACTTCTTCCCCGAGTCCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTCATCAGCCAGCG
CCTGGTGATCGGCAAGGACGGCCGCCGCGTGCCGGCCGTGGAAGTACTGCTGAACACCCCGCTGATCCGCGACATGATCC
GCCGCGGCCAGATCCACGAGGTGAAGGACGCCATGGATCGCAGCCTGCAGGAGGGCATGCAGACCTTCGACCAGTCGCTC
TACCGGCTCTACAAGGACGGGCAGATCACGCTGGACGAGTGCCTGGCCAAGGCCGATTCGCGTGACGGGCTGGCGTTGAA
GATCCGCCTGTCGGAGGGTGCGCCGGGGAATGAGTTTGCGTCGAGTGATCCTTATGGGATCGGGGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.829

98.103

0.499
  pilU Vibrio cholerae strain A1552

49.864

100

0.499
  pilU Acinetobacter baylyi ADP1

50.843

96.477

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.989

99.187

0.436
  pilT Acinetobacter baylyi ADP1

42.647

92.141

0.393
  pilT Pseudomonas stutzeri DSM 10701

41.42

91.599

0.379
  pilT Pseudomonas aeruginosa PAK

41.42

91.599

0.379
  pilT Legionella pneumophila strain Lp02

42.121

89.431

0.377
  pilT Acinetobacter nosocomialis M2

42.121

89.431

0.377
  pilT Acinetobacter baumannii D1279779

42.121

89.431

0.377
  pilT Acinetobacter baumannii strain A118

42.121

89.431

0.377
  pilT Legionella pneumophila strain ERS1305867

42.121

89.431

0.377
  pilT Neisseria meningitidis 8013

39.481

94.038

0.371
  pilT Neisseria gonorrhoeae MS11

39.481

94.038

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.356

91.328

0.369
  pilT Vibrio cholerae strain A1552

40.356

91.328

0.369