Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilT   Type   Machinery gene
Locus tag   KRO88_RS04695 Genome accession   NZ_CP076739
Coordinates   984372..985409 (-) Length   345 a.a.
NCBI ID   WP_000355489.1    Uniprot ID   A0A009PTR1
Organism   Acinetobacter baumannii strain X4-584     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 941951..1008851 984372..985409 within 0


Gene organization within MGE regions


Location: 941951..1008851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KRO88_RS04485 (KRO88_04455) - 942586..944211 (-) 1626 WP_000834616.1 NAD+ synthase -
  KRO88_RS04490 (KRO88_04460) - 944596..945156 (-) 561 WP_001048150.1 hypothetical protein -
  KRO88_RS04495 (KRO88_04465) - 945247..946107 (-) 861 WP_000838098.1 pirin family protein -
  KRO88_RS04500 (KRO88_04470) hemJ 946278..946730 (-) 453 WP_000339889.1 protoporphyrinogen oxidase HemJ -
  KRO88_RS04505 (KRO88_04475) - 946749..947978 (-) 1230 WP_000832710.1 beta-ketoacyl-ACP synthase II -
  KRO88_RS04510 (KRO88_04480) - 948275..949600 (+) 1326 WP_000160011.1 EcsC family protein -
  KRO88_RS04515 (KRO88_04485) rpsL 949801..950175 (+) 375 WP_000246374.1 30S ribosomal protein S12 -
  KRO88_RS04520 (KRO88_04490) rpsG 950335..950805 (+) 471 WP_001138055.1 30S ribosomal protein S7 -
  KRO88_RS04525 (KRO88_04495) fusA 950984..953122 (+) 2139 WP_000113824.1 elongation factor G -
  KRO88_RS04530 (KRO88_04500) tuf 953217..954407 (+) 1191 WP_001029610.1 elongation factor Tu -
  KRO88_RS04535 (KRO88_04505) - 954611..955492 (+) 882 WP_000992305.1 metal-dependent hydrolase -
  KRO88_RS04540 (KRO88_04510) - 955729..956604 (+) 876 WP_000992291.1 metal-dependent hydrolase -
  KRO88_RS04545 (KRO88_04515) rimI 956713..957168 (+) 456 WP_000621543.1 ribosomal protein S18-alanine N-acetyltransferase -
  KRO88_RS04550 (KRO88_04520) - 957213..958028 (-) 816 WP_000844343.1 arginyltransferase -
  KRO88_RS04555 (KRO88_04525) aat 958047..958778 (-) 732 WP_000854785.1 leucyl/phenylalanyl-tRNA--protein transferase -
  KRO88_RS04560 (KRO88_04530) trxB 958898..959845 (-) 948 WP_001276144.1 thioredoxin-disulfide reductase -
  KRO88_RS04565 (KRO88_04535) - 960115..963147 (+) 3033 WP_000127823.1 DNA translocase FtsK -
  KRO88_RS04570 (KRO88_04540) rhtC 963297..963917 (+) 621 WP_000959259.1 threonine export protein RhtC -
  KRO88_RS04575 (KRO88_04545) - 963973..964395 (-) 423 Protein_872 hypothetical protein -
  KRO88_RS04580 (KRO88_04550) - 964429..966075 (-) 1647 WP_001094416.1 IS66-like element ISAba17 family transposase -
  KRO88_RS04585 (KRO88_04555) tnpB 966150..966485 (-) 336 WP_000618090.1 IS66 family insertion sequence element accessory protein TnpB -
  KRO88_RS04590 (KRO88_04560) - 966482..966910 (-) 429 WP_000364662.1 transposase -
  KRO88_RS04595 - 966992..967120 (-) 129 Protein_876 hypothetical protein -
  KRO88_RS04600 (KRO88_04570) - 967532..967819 (-) 288 WP_001218560.1 PA4642 family protein -
  KRO88_RS04605 (KRO88_04575) minE 967903..968175 (-) 273 WP_000896934.1 cell division topological specificity factor MinE -
  KRO88_RS04610 (KRO88_04580) minD 968178..968990 (-) 813 WP_001074362.1 septum site-determining protein MinD -
  KRO88_RS04615 (KRO88_04585) minC 969061..969783 (-) 723 WP_000763677.1 septum site-determining protein MinC -
  KRO88_RS04620 (KRO88_04590) - 969905..970951 (-) 1047 WP_001181639.1 hypothetical protein -
  KRO88_RS04625 (KRO88_04595) - 970968..971894 (-) 927 WP_000100965.1 acyltransferase -
  KRO88_RS04630 (KRO88_04600) - 972139..972792 (+) 654 WP_001202415.1 OmpA family protein -
  KRO88_RS04635 (KRO88_04605) rep 972986..975025 (+) 2040 WP_000093035.1 DNA helicase Rep -
  KRO88_RS04640 (KRO88_04610) dut 975050..975502 (+) 453 WP_000868152.1 dUTP diphosphatase -
  KRO88_RS04645 (KRO88_04615) - 975702..977120 (+) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  KRO88_RS04650 (KRO88_04620) argB 977135..978043 (+) 909 WP_001135419.1 acetylglutamate kinase -
  KRO88_RS04655 (KRO88_04625) - 978164..978946 (+) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  KRO88_RS04660 (KRO88_04630) - 979055..979903 (+) 849 WP_000336548.1 class II glutamine amidotransferase -
  KRO88_RS04665 (KRO88_04635) - 980156..980611 (+) 456 WP_000782976.1 bacteriohemerythrin -
  KRO88_RS04670 (KRO88_04640) - 980764..981831 (+) 1068 WP_002019572.1 hypothetical protein -
  KRO88_RS04675 (KRO88_04645) - 981831..982154 (+) 324 WP_000442587.1 RnfH family protein -
  KRO88_RS04680 (KRO88_04650) bamE 982209..982607 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  KRO88_RS04685 (KRO88_04655) fur 982719..983156 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  KRO88_RS04690 (KRO88_04660) pilU 983226..984344 (-) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KRO88_RS04695 (KRO88_04665) pilT 984372..985409 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  KRO88_RS04700 (KRO88_04670) - 985536..986228 (+) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  KRO88_RS04705 (KRO88_04675) - 986430..990026 (-) 3597 WP_000698785.1 SbcC/MukB-like Walker B domain-containing protein -
  KRO88_RS04710 (KRO88_04680) - 990036..991304 (-) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  KRO88_RS04715 (KRO88_04685) - 991468..991941 (+) 474 WP_000974358.1 OsmC family protein -
  KRO88_RS04720 (KRO88_04690) - 992009..992386 (-) 378 WP_001059438.1 VOC family protein -
  KRO88_RS04725 (KRO88_04695) - 992766..993218 (+) 453 WP_000164153.1 ABZJ_00895 family protein -
  KRO88_RS04730 (KRO88_04700) - 993308..994213 (+) 906 WP_001039356.1 TIGR01777 family oxidoreductase -
  KRO88_RS04735 (KRO88_04705) - 994205..995320 (-) 1116 WP_000546701.1 FAD-dependent oxidoreductase -
  KRO88_RS04740 (KRO88_04710) hemB 995346..996359 (-) 1014 WP_000222569.1 porphobilinogen synthase -
  KRO88_RS04745 (KRO88_04715) - 996502..996999 (+) 498 WP_002019577.1 thioesterase family protein -
  KRO88_RS04750 (KRO88_04720) - 997269..998420 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  KRO88_RS04755 (KRO88_04725) - 998427..999950 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  KRO88_RS04760 (KRO88_04730) ggt 1000253..1002238 (+) 1986 WP_001042923.1 gamma-glutamyltransferase -
  KRO88_RS04770 (KRO88_04740) - 1002940..1004169 (+) 1230 WP_001991065.1 tyrosine-type recombinase/integrase -
  KRO88_RS04775 (KRO88_04745) - 1004317..1005084 (+) 768 WP_000578618.1 hypothetical protein -
  KRO88_RS04780 (KRO88_04750) - 1005294..1005509 (+) 216 WP_000153154.1 hypothetical protein -
  KRO88_RS04785 (KRO88_04755) - 1005512..1005721 (+) 210 WP_001016479.1 helix-turn-helix domain-containing protein -
  KRO88_RS04790 (KRO88_04760) - 1005718..1006272 (+) 555 WP_001988245.1 BRO family protein -
  KRO88_RS04795 (KRO88_04765) - 1006265..1006633 (+) 369 WP_001046481.1 hypothetical protein -
  KRO88_RS04800 - 1006643..1006945 (+) 303 Protein_916 hypothetical protein -
  KRO88_RS04805 (KRO88_04770) - 1007073..1007351 (+) 279 WP_001064707.1 hypothetical protein -

Sequence


Protein


Download         Length: 345 a.a.        Molecular weight: 38312.92 Da        Isoelectric Point: 6.7271

>NTDB_id=577904 KRO88_RS04695 WP_000355489.1 984372..985409(-) (pilT) [Acinetobacter baumannii strain X4-584]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=577904 KRO88_RS04695 WP_000355489.1 984372..985409(-) (pilT) [Acinetobacter baumannii strain X4-584]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTTTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCTCTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCCGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CAGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACTATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACACTACACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCAGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGTGGTGGCCGTGTCGCAGCACATGAGATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTACTCAGCCATTCAAACTGGTGCAAATCATGGCATGACCACCCTCGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTGATTAGCCCACAAACCGCACGTACTGCGGCAAAACAGCCTGAATCCTTCCTATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A009PTR1

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

100

100

1

  pilT Acinetobacter baumannii D1279779

100

100

1

  pilT Acinetobacter nosocomialis M2

99.71

100

0.997

  pilT Acinetobacter baylyi ADP1

88.953

99.71

0.887

  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841

  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838

  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754

  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754

  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699

  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699

  pilT Neisseria meningitidis 8013

68.116

100

0.681

  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678

  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522

  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409

  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394

  pilU Acinetobacter baylyi ADP1

38.728

100

0.388