NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	KP725_RS04295	Genome accession	NZ_CP076645
Coordinates	891791..892771 (+)	Length	326 a.a.
NCBI ID	WP_071999886.1	Uniprot ID	-
Organism	Escherichia coli strain MSI001
Function	type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 886791..897771

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KP725_RS04265 (KP725_04265)	yggM	887306..888313 (+)	1008	WP_000745244.1	DUF1202 family protein	-
KP725_RS04270 (KP725_04270)	hemW	888468..889604 (-)	1137	WP_032256677.1	radical SAM family heme chaperone HemW	-
KP725_RS04275 (KP725_04275)	rdgB	889597..890190 (-)	594	WP_001174735.1	XTP/dITP diphosphatase	-
KP725_RS04280 (KP725_04280)	yggU	890198..890488 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
KP725_RS04285 (KP725_04285)	yggT	890485..891051 (-)	567	WP_032237483.1	osmotic shock tolerance protein YggT	-
KP725_RS04290 (KP725_04290)	yggS	891069..891773 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
KP725_RS04295 (KP725_04295)	pilT	891791..892771 (+)	981	WP_071999886.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KP725_RS04300 (KP725_04300)	ruvX	892946..893362 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
KP725_RS04305 (KP725_04305)	yqgE	893362..893925 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
KP725_RS04310 (KP725_04310)	gshB	894034..894984 (-)	951	WP_000593273.1	glutathione synthase	-
KP725_RS04315 (KP725_04315)	rsmE	894997..895728 (-)	732	WP_001300912.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
KP725_RS04320 (KP725_04320)	endA	895808..896515 (-)	708	WP_000286500.1	deoxyribonuclease I	-
KP725_RS04325 (KP725_04325)	yggI	896610..897107 (-)	498	WP_216684233.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

Download Length: 326 a.a. Molecular weight: 35976.14 Da Isoelectric Point: 5.7980

                                >NTDB_id=576883 KP725_RS04295 WP_071999886.1 891791..892771(+) (pilT) [Escherichia coli strain MSI001]

MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG

SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE

YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS

FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR

VGEGRL

Nucleotide

Download Length: 981 bp

                                >NTDB_id=576883 KP725_RS04295 WP_071999886.1 891791..892771(+) (pilT) [Escherichia coli strain MSI001]

ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG

ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG

ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC

AGCGCATTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG

CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT

CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA

TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG

GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG

CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA

TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGGAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT

GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA

AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGA

GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	39.514	100	0.399
pilU	Pseudomonas stutzeri DSM 10701	38.554	100	0.393
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362

Detailed information NTG0576883