Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KMZ14_RS11900 Genome accession   NZ_CP076603
Coordinates   2460879..2461916 (+) Length   345 a.a.
NCBI ID   WP_163168563.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain MCDA01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2455879..2466916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KMZ14_RS11890 (KMZ14_11835) - 2458917..2460023 (+) 1107 WP_273797941.1 acyltransferase family protein -
  KMZ14_RS11895 (KMZ14_11840) - 2460069..2460755 (-) 687 WP_273799078.1 YggS family pyridoxal phosphate-dependent enzyme -
  KMZ14_RS11900 (KMZ14_11845) pilT 2460879..2461916 (+) 1038 WP_163168563.1 type IV pilus twitching motility protein PilT Machinery gene
  KMZ14_RS11905 (KMZ14_11850) pilU 2461947..2463068 (+) 1122 WP_048881346.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KMZ14_RS11910 (KMZ14_11855) fur 2463144..2463581 (-) 438 WP_004811125.1 ferric iron uptake transcriptional regulator -
  KMZ14_RS11915 (KMZ14_11860) - 2463694..2464092 (+) 399 WP_004811126.1 outer membrane protein assembly factor BamE -
  KMZ14_RS11920 (KMZ14_11865) - 2464202..2464528 (-) 327 WP_163171950.1 RnfH family protein -
  KMZ14_RS11925 (KMZ14_11870) - 2464528..2465607 (-) 1080 WP_273797942.1 hypothetical protein -
  KMZ14_RS11930 (KMZ14_11875) - 2465747..2466202 (-) 456 WP_273797943.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38223.80 Da        Isoelectric Point: 6.5056

>NTDB_id=576599 KMZ14_RS11900 WP_163168563.1 2460879..2461916(+) (pilT) [Acinetobacter schindleri strain MCDA01]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFKDICEYPRGLVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVVSQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGVISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=576599 KMZ14_RS11900 WP_163168563.1 2460879..2461916(+) (pilT) [Acinetobacter schindleri strain MCDA01]
ATGGACATTACAGAATTATTAGCATTTTCTGCTAAAAATGGCGCGTCAGATTTACACTTGTCTGCGGGGATGCCACCACT
GATTCGTGTTGATGGTGAAGTTCGTCGTATTAACCTGCCTGCCCTGGAACATAAAGAAGTGCACAAGCTGGTTTACGATA
TCATGAACGATAAACAGCGCCGTGACTATGAAGAAAATCTAGAAACTGACTTTTCCTTTGAAGTTCCTGGTGTTGCCCGT
TTCCGTGTGAACGCATTTAATCAGAACCGTGGTGCCGGTGCCGTATTCCGTACCATTCCCTCTAAAGTTTTAACCATTGA
AGATCTGGGCATGGGTCAGATCTTTAAAGATATTTGTGAATATCCGCGTGGTCTGGTACTGGTCACTGGTCCAACCGGTT
CAGGTAAATCAACAACACTGGCAGCGATGTTGGACTATATCAATGATAACCGTTATGACCATATTCTGACCGTCGAAGAT
CCAATTGAATTTGTACACCAATCCAAAAAGTGTCTGATCAACCAGCGTGAAGTGCATCGTGACACGCATGGTTTCAATGA
AGCATTACGTTCTGCATTACGTGAAGACCCGGATATTATCCTGGTGGGTGAGATGCGTGACCTTGAAACCATCCGTCTGG
CACTCACTGCTGCTGAAACCGGCCACCTGGTGTTTGGCACCCTGCATACGACTTCTGCTGCTAAAACTATTGACCGTGTA
ATCGACGTATTCCCGGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCATTACAAGCAGTCGTTTCACAAAC
CCTGCTCAAGAAAAATGGTGGTGGTCGTGTCGCTGCACATGAAATCATGATCGGTATTCCGGCCATCCGTAACCTGATCC
GTGAAAACAAAGTTGCTCAAATGTACTCTGCCATTCAGACCGGTGCCAACTATGGTATGACTACGCTGGATCAGAGCCTG
AAAACACTGGTATCTAAAGGCGTGATCAGTCCACAAGTTGCGCGTACTGCTGCTAAACAGCCTGAATCTTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

95.362

100

0.954
  pilT Acinetobacter baumannii D1279779

95.072

100

0.951
  pilT Acinetobacter baumannii strain A118

95.072

100

0.951
  pilT Acinetobacter baylyi ADP1

86.919

99.71

0.867
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Neisseria meningitidis 8013

68.406

100

0.684
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.88

96.232

0.672
  pilT Vibrio cholerae strain A1552

69.88

96.232

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.692

95.942

0.4
  pilU Acinetobacter baylyi ADP1

38.15

100

0.383