Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KP625_RS00860 Genome accession   NZ_CP076562
Coordinates   191292..192344 (+) Length   350 a.a.
NCBI ID   WP_177970988.1    Uniprot ID   -
Organism   Eubacterium sp. MSJ-33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 186292..197344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KP625_RS00840 (KP625_00840) - 186822..187922 (+) 1101 WP_238298717.1 PD-(D/E)XK nuclease family transposase -
  KP625_RS00845 (KP625_00845) sigK 187990..188613 (-) 624 WP_238298719.1 RNA polymerase sporulation sigma factor SigK -
  KP625_RS00850 (KP625_00850) - 188732..189418 (+) 687 WP_238298721.1 CPBP family intramembrane glutamic endopeptidase -
  KP625_RS00855 (KP625_00855) - 189528..191255 (+) 1728 WP_238298723.1 GspE/PulE family protein -
  KP625_RS00860 (KP625_00860) pilT 191292..192344 (+) 1053 WP_177970988.1 type IV pilus twitching motility protein PilT Machinery gene
  KP625_RS00865 (KP625_00865) - 192483..193685 (+) 1203 WP_238298725.1 type II secretion system F family protein -
  KP625_RS00870 (KP625_00870) - 193769..194389 (+) 621 WP_238298727.1 type II secretion system protein -
  KP625_RS00875 (KP625_00875) - 194493..195245 (+) 753 WP_370641380.1 A24 family peptidase -
  KP625_RS00880 (KP625_00880) pilM 195302..196942 (+) 1641 WP_238298729.1 type IV pilus assembly protein PilM -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38657.57 Da        Isoelectric Point: 7.0425

>NTDB_id=576397 KP625_RS00860 WP_177970988.1 191292..192344(+) (pilT) [Eubacterium sp. MSJ-33]
MIDMKELLAFAVEQNASDIHIATGIPPKLRINGRLTEVELPPMTPADAAEAIGSTMHDRHKQILKERGEVDFSFSSPETG
RFRVNVFMDRGNMAAAYRKVDTIIPRPDQLGIPEAVVQLYKKKRGLVLVTGPTGSGKSTTLASIINKANENLTDHIITLE
DPIEYIHKHKKSVVNQREVGMDTLSYANALRAALREDPDIILVGEMRDLETISTAITAAETGHLVFSTLHTIGAASTIDR
IIDVFPTHQQQQTRIQLAMILEGVISQALIPTADGKGRVAAFEVMLASPAIRSLIREQKNHQIQSTIQTSRAQGMITLDD
FILDLYKSGKITRDMALVYAQDQVAMKKQM

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=576397 KP625_RS00860 WP_177970988.1 191292..192344(+) (pilT) [Eubacterium sp. MSJ-33]
ATGATAGACATGAAAGAACTTTTGGCATTCGCGGTAGAACAGAATGCATCGGATATCCATATTGCGACAGGGATTCCGCC
GAAACTCCGTATCAATGGCCGGCTGACAGAAGTAGAGTTACCGCCGATGACGCCGGCAGATGCGGCAGAGGCGATTGGGT
CGACGATGCATGACCGTCACAAACAGATCTTGAAAGAGAGAGGTGAGGTCGACTTCTCCTTCTCATCTCCGGAGACAGGC
CGATTCCGTGTGAATGTTTTCATGGACCGTGGCAATATGGCGGCTGCATACAGAAAGGTTGATACGATTATTCCAAGACC
GGATCAGCTAGGTATTCCGGAGGCGGTCGTACAGTTATATAAGAAGAAAAGAGGTCTGGTACTCGTAACAGGTCCGACAG
GTTCCGGTAAATCAACGACACTTGCTTCTATTATTAATAAAGCAAATGAAAACCTGACAGACCATATCATTACACTGGAG
GATCCGATCGAGTATATCCACAAACATAAGAAGTCGGTTGTAAACCAGCGAGAGGTTGGTATGGATACGCTCTCTTATGC
GAATGCTCTTCGTGCAGCACTTCGTGAGGATCCGGATATTATTCTGGTAGGAGAGATGCGTGACTTGGAGACAATCTCTA
CAGCAATCACAGCTGCTGAGACCGGACATCTGGTGTTTTCAACACTGCATACAATCGGTGCTGCATCCACGATCGACCGT
ATCATCGATGTGTTCCCGACACATCAGCAGCAACAGACACGTATCCAGTTAGCGATGATTCTGGAAGGCGTTATCTCGCA
GGCACTGATTCCGACAGCAGACGGAAAAGGACGAGTAGCGGCATTTGAGGTCATGCTTGCCAGCCCGGCGATTCGAAGCC
TGATTCGTGAGCAGAAGAACCATCAGATTCAGTCTACGATTCAGACAAGCCGTGCACAGGGCATGATTACATTGGATGAT
TTTATCTTAGATCTCTACAAGAGTGGTAAGATCACAAGAGATATGGCGCTTGTATACGCACAGGATCAGGTCGCAATGAA
GAAACAGATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.44

97.429

0.491
  pilT Neisseria meningitidis 8013

49.275

98.571

0.486
  pilT Neisseria gonorrhoeae MS11

48.986

98.571

0.483
  pilT Legionella pneumophila strain ERS1305867

50.448

95.714

0.483
  pilT Pseudomonas aeruginosa PAK

50.448

95.714

0.483
  pilT Pseudomonas stutzeri DSM 10701

50.448

95.714

0.483
  pilT Legionella pneumophila strain Lp02

50.448

95.714

0.483
  pilT Acinetobacter baylyi ADP1

51.057

94.571

0.483
  pilT Vibrio cholerae strain A1552

52.83

90.857

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.83

90.857

0.48
  pilT Acinetobacter nosocomialis M2

49.118

97.143

0.477
  pilT Acinetobacter baumannii D1279779

49.849

94.571

0.471
  pilT Acinetobacter baumannii strain A118

49.849

94.571

0.471
  pilU Pseudomonas stutzeri DSM 10701

40.708

96.857

0.394
  pilU Acinetobacter baylyi ADP1

40.588

97.143

0.394
  pilU Vibrio cholerae strain A1552

40.476

96

0.389