Detailed information NTG0057393

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: HYDSN_RS01245
• Genome accession: NC_020814
• Coordinates: 212616..213695 (-)
• Length: 359 a.a.
• NCBI ID: WP_015418884.1
• Uniprot ID: M1RBV6
• Organism: Hydrogenobaculum sp. SN
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 207616..218695

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HYDSN_RS01220 (HydSN_0247)	-	208061..208276 (-)	216	WP_015418878.1	hypothetical protein	-
HYDSN_RS01225 (HydSN_0248)	-	208273..209184 (-)	912	WP_015418879.1	MoxR family ATPase	-
HYDSN_RS01230 (HydSN_0249)	-	209205..211127 (-)	1923	WP_015418880.1	GGDEF domain-containing protein	-
HYDSN_RS01235 (HydSN_0251)	-	211422..211718 (+)	297	WP_237698614.1	hypothetical protein	-
HYDSN_RS01240 (HydSN_0252)	recR	211708..212307 (+)	600	WP_015418883.1	recombination mediator RecR	-
HYDSN_RS01245 (HydSN_0253)	pilT	212616..213695 (-)	1080	WP_015418884.1	type IV pilus twitching motility protein PilT	Machinery gene
HYDSN_RS01250 (HydSN_0254)	-	213711..215390 (-)	1680	WP_015418885.1	GspE/PulE family protein	-

Sequence

Protein

• Length: 359 a.a.
• Molecular weight: 40116.43 Da
• Isoelectric Point: 6.8596

>NTDB_id=57393 HYDSN_RS01245 WP_015418884.1 212616..213695(-) (pilT) [Hydrogenobaculum sp. SN]
MQLDIGTLTEILVKNRGSDIHIVAGSKPAIRVDGKIRFLEEYDTLTPDDTQMLAYGIMLEKYKKVFEEKNAVDFSIGIHS
LGRFRVNVFRQRDSISMVLRYLSSDIKDITKLGLEAKVLELTKRSMGLVLVTGPTGSGKSTTLASMIQYILDNEPAHVIT
VEDPIEYLFKHSKGIVNQRELGQDVPSFSDALRSALREDPDVILIGEMRDRETIEMALRAAETGHLVFGTLHTNTAISTI
NRIVDVFSAEEKDQIRTQLSISLQGIISQRLLPKIGGGRVLAYELLIPNAGIRNLIKENKLPQIYALMQSGQAQTGMQTM
NQHLRKLYGMGLITKEDALKYSPDPKELARLMGVAYEEI

Nucleotide

• Length: 1080 bp

>NTDB_id=57393 HYDSN_RS01245 WP_015418884.1 212616..213695(-) (pilT) [Hydrogenobaculum sp. SN]
ATGCAGCTTGACATAGGAACGTTAACGGAGATACTTGTAAAAAATAGAGGAAGCGATATCCATATAGTAGCAGGCTCTAA
ACCTGCCATAAGAGTGGATGGCAAAATAAGGTTTTTAGAAGAATACGATACATTAACACCTGACGATACGCAAATGCTGG
CATACGGCATTATGCTTGAAAAATATAAAAAAGTATTTGAAGAAAAAAATGCGGTAGATTTTTCTATCGGAATACACAGT
TTAGGCAGATTTCGTGTAAACGTGTTTAGACAAAGAGACTCCATAAGCATGGTTTTAAGATACTTATCCAGCGATATAAA
AGATATTACAAAATTAGGATTAGAAGCAAAGGTATTAGAGCTTACAAAAAGGAGTATGGGACTTGTTTTAGTAACAGGTC
CAACCGGCAGTGGTAAATCAACGACCTTAGCATCGATGATACAATATATACTTGACAACGAACCAGCACATGTTATAACA
GTGGAAGACCCCATAGAATACCTTTTCAAACACAGCAAAGGCATAGTAAACCAACGCGAGTTAGGGCAAGACGTGCCAAG
TTTTTCCGATGCTTTAAGAAGCGCTTTAAGAGAAGACCCAGACGTAATATTAATAGGTGAAATGAGAGATAGAGAAACTA
TAGAAATGGCTTTAAGAGCAGCTGAAACCGGACACTTGGTATTTGGAACGCTTCATACAAATACAGCTATATCCACTATA
AACAGAATTGTAGATGTATTTAGCGCTGAAGAAAAGGATCAAATAAGGACGCAGCTCTCTATCTCTTTACAAGGTATCAT
ATCTCAAAGGCTTTTGCCTAAAATAGGCGGGGGCAGAGTGTTAGCTTACGAGCTTTTAATACCAAACGCAGGTATAAGAA
ACTTAATAAAAGAAAATAAGCTACCACAAATTTATGCATTGATGCAATCAGGTCAAGCTCAAACAGGTATGCAAACTATG
AACCAACACTTAAGAAAACTATACGGTATGGGGCTTATAACAAAAGAAGATGCTCTAAAATACAGTCCAGATCCAAAAGA
ATTGGCAAGGTTAATGGGTGTAGCTTACGAAGAGATATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (10-273)

3D structure

• AlphaFold DB: M1RBV6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Legionella pneumophila strain Lp02	52.083	93.593	0.487
pilT	Legionella pneumophila strain ERS1305867	52.083	93.593	0.487
pilT	Acinetobacter baylyi ADP1	50	96.379	0.482
pilT	Acinetobacter baumannii D1279779	49.711	96.379	0.479
pilT	Acinetobacter baumannii strain A118	49.711	96.379	0.479
pilT	Acinetobacter nosocomialis M2	49.711	96.379	0.479
pilT	Pseudomonas aeruginosa PAK	48.844	96.379	0.471
pilT	Pseudomonas stutzeri DSM 10701	48.266	96.379	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	47.714	97.493	0.465
pilT	Neisseria meningitidis 8013	46.552	96.936	0.451
pilT	Vibrio cholerae strain A1552	47.463	93.315	0.443
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	47.463	93.315	0.443
pilT	Neisseria gonorrhoeae MS11	47.041	94.15	0.443
pilU	Pseudomonas stutzeri DSM 10701	41.643	98.329	0.409
pilU	Acinetobacter baylyi ADP1	39.368	96.936	0.382
pilU	Vibrio cholerae strain A1552	38.596	95.265	0.368