Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A11Q_RS12865 Genome accession   NC_020813
Coordinates   2585367..2586404 (+) Length   345 a.a.
NCBI ID   WP_015471261.1    Uniprot ID   M4VFE1
Organism   Pseudobdellovibrio exovorus JSS     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2580367..2591404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A11Q_RS12840 (A11Q_2550) - 2580719..2581288 (-) 570 WP_015471256.1 hypothetical protein -
  A11Q_RS12845 (A11Q_2551) - 2581365..2581988 (+) 624 WP_015471257.1 hypothetical protein -
  A11Q_RS12850 (A11Q_2552) - 2582007..2582321 (-) 315 WP_015471258.1 YbjQ family protein -
  A11Q_RS12855 (A11Q_2553) - 2582379..2583602 (-) 1224 WP_015471259.1 hypothetical protein -
  A11Q_RS12860 (A11Q_2554) - 2583662..2585182 (-) 1521 WP_015471260.1 Rne/Rng family ribonuclease -
  A11Q_RS12865 (A11Q_2555) pilT 2585367..2586404 (+) 1038 WP_015471261.1 type IV pilus twitching motility protein PilT Machinery gene
  A11Q_RS12870 (A11Q_2556) deoC 2586471..2587166 (+) 696 WP_015471262.1 deoxyribose-phosphate aldolase -
  A11Q_RS12875 (A11Q_2557) - 2587265..2588581 (+) 1317 WP_015471263.1 thymidine phosphorylase -
  A11Q_RS12880 (A11Q_2558) - 2588608..2589423 (+) 816 WP_015471264.1 purine-nucleoside phosphorylase -
  A11Q_RS12885 (A11Q_2559) - 2589801..2591105 (+) 1305 WP_015471265.1 S8 family peptidase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37857.78 Da        Isoelectric Point: 7.6210

>NTDB_id=57389 A11Q_RS12865 WP_015471261.1 2585367..2586404(+) (pilT) [Pseudobdellovibrio exovorus JSS]
MATIDDLFRLMVEQKASDLHLTSGAPPFLRLHGNMSPLNYRQLSNQDVQGLIFEILTEKQKKAFVEKWELDFAYVVEGLG
RFRCNVFMQRKGLGAVFRTIPEKIKTAQELNLPPAIIDLVDADRGLILVTGPTGSGKSTTLAAMIQHINATREAHILTVE
DPIEFVHPNLKSLVNQREVGSHTKTFANALKAALREDPDIILVGELRDLETISLALTAAETGHLVFGTLHTSSAAKTVDR
IIDVFPQGQQGQIRTMLSESIRGIVAQSLFTRADGQGRVAAFEIMKGTKAIGNLIREGKIHQIPSIIQTSAAHGMVLYEK
YVDDLVKKGLITLADAKTFLGKDAA

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=57389 A11Q_RS12865 WP_015471261.1 2585367..2586404(+) (pilT) [Pseudobdellovibrio exovorus JSS]
ATGGCTACTATCGATGATCTTTTTAGATTAATGGTCGAACAAAAAGCATCAGATTTACACTTAACAAGTGGCGCTCCTCC
GTTTCTGCGTTTACACGGCAACATGTCACCGCTGAATTACCGCCAATTAAGCAATCAAGATGTTCAAGGTCTTATTTTCG
AAATCCTAACTGAAAAGCAAAAGAAAGCCTTCGTTGAAAAATGGGAGCTCGATTTCGCCTACGTTGTCGAAGGGCTCGGG
CGATTCCGCTGTAACGTCTTCATGCAACGTAAAGGTTTAGGAGCCGTTTTCAGAACGATTCCTGAAAAAATTAAAACCGC
ACAAGAGTTAAATTTGCCTCCGGCTATTATCGATTTAGTCGATGCGGATCGCGGTTTGATTTTGGTTACAGGTCCAACAG
GTTCAGGTAAGTCGACAACGCTGGCTGCGATGATTCAACATATCAACGCCACTCGCGAAGCACATATTCTGACGGTAGAA
GATCCTATCGAGTTCGTGCATCCAAATTTAAAATCATTAGTGAATCAACGTGAAGTTGGCTCGCATACAAAAACTTTTGC
CAACGCTTTAAAAGCCGCACTTCGTGAAGATCCAGATATCATTCTGGTGGGTGAGCTTCGTGACTTAGAAACAATTTCAC
TGGCGTTGACCGCAGCCGAAACGGGACACTTAGTTTTCGGAACACTTCATACCAGCTCGGCGGCGAAAACCGTGGATCGT
ATTATCGACGTTTTCCCACAGGGCCAACAAGGTCAGATCCGTACGATGTTATCGGAGTCTATCCGCGGGATAGTAGCTCA
ATCTTTGTTTACTCGTGCTGACGGACAGGGCCGTGTTGCTGCTTTTGAAATCATGAAGGGAACCAAAGCCATTGGGAACT
TAATCCGTGAAGGTAAGATTCACCAAATCCCATCTATTATTCAAACCAGCGCTGCCCATGGCATGGTGCTTTATGAAAAA
TACGTGGACGACCTTGTTAAAAAGGGTCTTATCACATTAGCCGATGCCAAAACTTTCTTAGGAAAAGACGCGGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M4VFE1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

56.886

96.812

0.551
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

56.707

95.072

0.539
  pilT Vibrio cholerae strain A1552

56.707

95.072

0.539
  pilT Acinetobacter nosocomialis M2

55.224

97.101

0.536
  pilT Acinetobacter baumannii D1279779

55.224

97.101

0.536
  pilT Acinetobacter baumannii strain A118

55.224

97.101

0.536
  pilT Pseudomonas aeruginosa PAK

55.389

96.812

0.536
  pilT Pseudomonas stutzeri DSM 10701

55.09

96.812

0.533
  pilT Legionella pneumophila strain ERS1305867

55.623

95.362

0.53
  pilT Legionella pneumophila strain Lp02

55.623

95.362

0.53
  pilT Neisseria gonorrhoeae MS11

52.994

96.812

0.513
  pilT Neisseria meningitidis 8013

53.659

95.072

0.51
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.191

95.362

0.507
  pilU Acinetobacter baylyi ADP1

41.742

96.522

0.403
  pilU Vibrio cholerae strain A1552

42.724

93.623

0.4
  pilU Pseudomonas stutzeri DSM 10701

41.265

96.232

0.397

Multiple sequence alignment