Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PCNPT3_RS01675 Genome accession   NC_020802
Coordinates   369141..370175 (+) Length   344 a.a.
NCBI ID   WP_015464140.1    Uniprot ID   M4U1X7
Organism   Psychromonas sp. CNPT3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 364141..375175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCNPT3_RS01640 (PCNPT3_01550) hemW 364352..365491 (-) 1140 WP_015464133.1 radical SAM family heme chaperone HemW -
  PCNPT3_RS01645 (PCNPT3_01555) - 365658..366260 (-) 603 WP_015464134.1 XTP/dITP diphosphatase -
  PCNPT3_RS01650 (PCNPT3_01560) - 366272..366700 (-) 429 WP_015464135.1 DUF4426 domain-containing protein -
  PCNPT3_RS01655 (PCNPT3_01565) yggU 366693..366992 (-) 300 WP_156801483.1 DUF167 family protein YggU -
  PCNPT3_RS01660 (PCNPT3_01570) - 366992..367534 (-) 543 WP_015464137.1 YggT family protein -
  PCNPT3_RS01665 (PCNPT3_01575) proC 367560..368384 (-) 825 WP_015464138.1 pyrroline-5-carboxylate reductase -
  PCNPT3_RS01670 (PCNPT3_01580) - 368424..369122 (-) 699 WP_015464139.1 YggS family pyridoxal phosphate-dependent enzyme -
  PCNPT3_RS01675 (PCNPT3_01585) pilT 369141..370175 (+) 1035 WP_015464140.1 type IV pilus twitching motility protein PilT Machinery gene
  PCNPT3_RS01680 (PCNPT3_01590) yaaA 370362..371138 (+) 777 WP_015464141.1 peroxide stress protein YaaA -
  PCNPT3_RS01685 (PCNPT3_01595) srmB 371180..372577 (-) 1398 WP_015464142.1 ATP-dependent RNA helicase SrmB -
  PCNPT3_RS01690 (PCNPT3_01600) - 372761..373075 (-) 315 WP_015464143.1 helix-hairpin-helix domain-containing protein -
  PCNPT3_RS01695 (PCNPT3_01605) - 373255..373941 (-) 687 WP_015464144.1 energy transducer TonB -
  PCNPT3_RS01700 (PCNPT3_01610) - 373938..374345 (-) 408 WP_015464145.1 biopolymer transporter ExbD -
  PCNPT3_RS01705 (PCNPT3_01615) - 374342..375055 (-) 714 WP_015464146.1 MotA/TolQ/ExbB proton channel family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38133.12 Da        Isoelectric Point: 6.1772

>NTDB_id=57364 PCNPT3_RS01675 WP_015464140.1 369141..370175(+) (pilT) [Psychromonas sp. CNPT3]
MDITELLAFSVKSNASDLHLSAGLVPMIRVDGEIRKINIAALAQKDVKALVYDIMTDKQRKDYEMNLEVDFSFEIPDLAR
FRVNVFTQERGMSGVFRTIPSKVMSLEELNAPEIFQKIANFPRGLVLVTGPTGSGKSTTLAAIIDYINITKNEHILTIED
PIEFVHESKGCLINQREVYRDTLSFDNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKSVDRI
IDVFPAAEKEMVRSMLSESLRAVISQTLLKKPAGGRVAAHEIMIGIPAIRNLIREDKVAQMYSVIQTGMIHGMQTLDQCL
RELVNQGVISNGVARQKAADPKTF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=57364 PCNPT3_RS01675 WP_015464140.1 369141..370175(+) (pilT) [Psychromonas sp. CNPT3]
ATGGATATTACTGAATTATTAGCTTTTAGTGTAAAGTCTAATGCTTCTGATTTACACCTTTCTGCTGGGCTAGTTCCCAT
GATCAGGGTCGATGGTGAAATACGCAAGATAAATATAGCCGCTTTAGCGCAAAAAGATGTAAAAGCACTGGTTTATGACA
TTATGACAGATAAACAGCGCAAAGATTATGAAATGAATTTAGAGGTCGATTTTTCTTTTGAAATCCCTGATTTGGCGCGT
TTTCGTGTGAATGTGTTCACCCAAGAGCGAGGCATGAGTGGGGTGTTTCGTACTATCCCCAGTAAAGTAATGTCCCTTGA
AGAATTAAATGCCCCTGAAATATTCCAAAAAATAGCTAATTTCCCGCGTGGATTGGTCTTAGTGACAGGGCCAACGGGCT
CAGGTAAATCTACGACGTTAGCAGCTATCATTGATTATATTAATATAACAAAAAACGAACACATTTTGACCATTGAAGAT
CCCATTGAATTTGTGCATGAAAGTAAAGGTTGTTTAATCAATCAACGTGAAGTTTATCGAGATACATTAAGTTTTGATAA
CGCGCTAAGAAGCGCATTACGTGAAGATCCCGATGTTATTTTGGTGGGGGAATTACGCGACCTTGAAACGATCCGCTTAG
CATTAACTGCTGCTGAAACTGGGCATCTGGTATTTGGTACGTTACATACGACCTCTGCAGCTAAAAGTGTGGATCGTATT
ATAGATGTTTTCCCCGCTGCTGAAAAAGAGATGGTGAGATCGATGCTATCTGAATCATTACGCGCGGTTATCTCGCAAAC
ATTACTTAAAAAACCTGCGGGTGGGCGTGTGGCAGCCCATGAGATCATGATTGGTATCCCTGCTATTCGTAACTTAATTC
GTGAAGATAAAGTGGCGCAAATGTATTCTGTTATTCAAACCGGGATGATCCATGGCATGCAAACGCTGGATCAATGTTTA
CGTGAACTTGTAAACCAAGGTGTTATCTCTAACGGAGTGGCACGTCAAAAAGCAGCCGATCCTAAAACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M4U1X7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

75.291

100

0.753
  pilT Acinetobacter baumannii strain A118

75.291

100

0.753
  pilT Acinetobacter nosocomialis M2

75.291

100

0.753
  pilT Pseudomonas stutzeri DSM 10701

75

100

0.75
  pilT Pseudomonas aeruginosa PAK

75

100

0.75
  pilT Acinetobacter baylyi ADP1

74.709

100

0.747
  pilT Legionella pneumophila strain Lp02

70.93

100

0.709
  pilT Legionella pneumophila strain ERS1305867

70.93

100

0.709
  pilT Neisseria meningitidis 8013

68.986

100

0.692
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.733

95.64

0.686
  pilT Vibrio cholerae strain A1552

71.733

95.64

0.686
  pilT Neisseria gonorrhoeae MS11

68.406

100

0.686
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Acinetobacter baylyi ADP1

40.634

100

0.41
  pilU Vibrio cholerae strain A1552

41.692

96.221

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.964

96.512

0.395

Multiple sequence alignment