Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CLOAM_RS06620 Genome accession   NC_020449
Coordinates   1636135..1637190 (-) Length   351 a.a.
NCBI ID   WP_015425110.1    Uniprot ID   B0VJ51
Organism   Candidatus Cloacimonas acidaminovorans str. Evry     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1631135..1642190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLOAM_RS06595 (CLOAM1395) - 1631299..1631892 (-) 594 WP_015425105.1 hypothetical protein -
  CLOAM_RS06600 (CLOAM1396) pilM 1631908..1632978 (-) 1071 WP_015425106.1 type IV pilus assembly protein PilM -
  CLOAM_RS06605 (CLOAM1397) - 1633020..1634978 (-) 1959 WP_015425107.1 hypothetical protein -
  CLOAM_RS06610 (CLOAM1398) - 1634998..1635648 (-) 651 WP_232502664.1 type II secretion system protein -
  CLOAM_RS06615 (CLOAM1399) - 1635656..1636132 (-) 477 WP_015425109.1 hypothetical protein -
  CLOAM_RS06620 (CLOAM1400) pilT 1636135..1637190 (-) 1056 WP_015425110.1 type IV pilus twitching motility protein PilT Machinery gene
  CLOAM_RS06625 (CLOAM1401) - 1637210..1637647 (-) 438 WP_015425111.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CLOAM_RS06630 (CLOAM1402) - 1637830..1639869 (+) 2040 WP_015425112.1 peptidyl-prolyl cis-trans isomerase -
  CLOAM_RS06635 (CLOAM1403) - 1640368..1641222 (-) 855 WP_044279029.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39097.76 Da        Isoelectric Point: 6.3470

>NTDB_id=56449 CLOAM_RS06620 WP_015425110.1 1636135..1637190(-) (pilT) [Candidatus Cloacimonas acidaminovorans str. Evry]
MTIHELLRFTAEAGASDLHIAAGSHPMVRVNGRMKKLNLPIQSPEEVETLVYGVMNESQQALFKKNLEIDFSTKLSNDVR
FRVNAFHQINGVSCAFRVIPNEIKSYEELHLPDILKRLTTKEKGLILVTGPTGSGKSTTLATMIDTINDSRNCHIITIED
PIEFVHRSKNSLINQRELGHDTWSFTAALRSALREDPDVILVGEMRDLETVSLALTAAETGHLVFATLHTGSCTKSIDRI
IDMFPKEQQQQVRSMLSESLEAVIAQTLLPTKDGKGRVPAVEIMIANAAVRNLIREEKTYQIPSIIQASTKEGMQTRDQS
LYNLVMNNLIERTIAEEFADNPKQFATGAGF

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=56449 CLOAM_RS06620 WP_015425110.1 1636135..1637190(-) (pilT) [Candidatus Cloacimonas acidaminovorans str. Evry]
TTGACAATCCATGAATTACTGCGCTTTACTGCAGAAGCAGGTGCGTCTGACCTTCATATAGCGGCTGGCTCACATCCTAT
GGTTCGGGTAAATGGCAGAATGAAAAAATTAAATCTGCCAATTCAAAGTCCGGAAGAGGTGGAAACACTTGTTTACGGAG
TGATGAACGAATCACAGCAAGCTCTCTTTAAAAAGAATCTGGAAATTGACTTTTCCACGAAATTGAGCAATGATGTCCGA
TTCAGAGTGAACGCTTTTCACCAGATAAATGGTGTTTCCTGTGCTTTCAGAGTGATTCCTAATGAGATAAAAAGTTATGA
GGAACTCCATCTGCCCGATATCTTAAAGCGCTTAACTACAAAAGAAAAAGGGCTGATTCTGGTTACAGGACCTACGGGAA
GCGGAAAATCAACTACTTTGGCTACAATGATTGATACTATAAATGATAGCCGTAACTGCCATATTATTACTATTGAAGAC
CCTATTGAATTTGTCCACCGCAGTAAAAACAGCTTAATCAATCAGCGTGAACTGGGTCATGATACCTGGAGCTTTACAGC
TGCCTTAAGAAGTGCCTTGCGTGAAGACCCGGATGTGATTTTAGTCGGTGAAATGCGTGATCTGGAGACGGTATCTTTAG
CATTAACAGCTGCTGAAACAGGTCACTTGGTTTTTGCTACTTTGCATACCGGAAGCTGCACAAAATCCATAGACCGGATA
ATAGATATGTTTCCTAAGGAACAACAACAGCAGGTGCGTTCAATGCTTTCGGAATCCCTGGAAGCGGTTATAGCACAAAC
TTTGTTACCTACCAAAGATGGTAAAGGTCGTGTTCCGGCTGTAGAAATAATGATTGCTAATGCTGCTGTGCGAAATCTGA
TCCGTGAAGAAAAAACCTATCAGATACCGTCTATAATTCAAGCAAGCACCAAGGAAGGAATGCAAACCAGAGATCAATCT
TTGTATAACCTGGTAATGAATAATCTGATTGAAAGAACAATTGCGGAAGAATTTGCGGATAATCCTAAACAATTTGCTAC
AGGAGCAGGTTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0VJ51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

58.092

98.575

0.573
  pilT Neisseria gonorrhoeae MS11

57.803

98.575

0.57
  pilT Legionella pneumophila strain Lp02

57.681

98.291

0.567
  pilT Legionella pneumophila strain ERS1305867

57.681

98.291

0.567
  pilT Acinetobacter nosocomialis M2

56.812

98.291

0.558
  pilT Acinetobacter baumannii D1279779

56.812

98.291

0.558
  pilT Acinetobacter baumannii strain A118

56.812

98.291

0.558
  pilT Pseudomonas aeruginosa PAK

56.522

98.291

0.556
  pilT Acinetobacter baylyi ADP1

56.232

98.291

0.553
  pilT Pseudomonas stutzeri DSM 10701

55.942

98.291

0.55
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

57.1

94.302

0.538
  pilT Vibrio cholerae strain A1552

57.1

94.302

0.538
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.326

98.006

0.513
  pilU Pseudomonas stutzeri DSM 10701

45.161

97.151

0.439
  pilU Acinetobacter baylyi ADP1

41.108

97.721

0.402
  pilU Vibrio cholerae strain A1552

41.896

93.162

0.39

Multiple sequence alignment