Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KGP22_RS18475 Genome accession   NZ_CP074159
Coordinates   3908647..3909627 (-) Length   326 a.a.
NCBI ID   WP_149367656.1    Uniprot ID   -
Organism   Enterobacter sp. JBIWA005     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3903647..3914627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KGP22_RS18445 (KGP22_18350) - 3904523..3905020 (+) 498 WP_025757721.1 SprT family zinc-dependent metalloprotease -
  KGP22_RS18450 (KGP22_18355) endA 3905115..3905822 (+) 708 WP_115876242.1 deoxyribonuclease I -
  KGP22_RS18455 (KGP22_18360) rsmE 3905874..3906605 (+) 732 WP_149367659.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KGP22_RS18460 (KGP22_18365) gshB 3906625..3907572 (+) 948 WP_149367658.1 glutathione synthase -
  KGP22_RS18465 (KGP22_18370) - 3907660..3908220 (+) 561 WP_020883695.1 YqgE/AlgH family protein -
  KGP22_RS18470 (KGP22_18375) ruvX 3908220..3908636 (+) 417 WP_149367657.1 Holliday junction resolvase RuvX -
  KGP22_RS18475 (KGP22_18380) pilT 3908647..3909627 (-) 981 WP_149367656.1 type IV pilus twitching motility protein PilT Machinery gene
  KGP22_RS18480 (KGP22_18385) - 3909645..3910346 (+) 702 WP_111963225.1 YggS family pyridoxal phosphate-dependent enzyme -
  KGP22_RS18485 (KGP22_18390) - 3910368..3910934 (+) 567 WP_111963226.1 YggT family protein -
  KGP22_RS18490 (KGP22_18395) yggU 3910931..3911227 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  KGP22_RS18495 (KGP22_18400) - 3911231..3911824 (+) 594 WP_149367655.1 XTP/dITP diphosphatase -
  KGP22_RS18500 (KGP22_18405) hemW 3911817..3912959 (+) 1143 WP_223580468.1 radical SAM family heme chaperone HemW -
  KGP22_RS18505 (KGP22_18410) - 3913133..3913849 (-) 717 WP_008499762.1 DUF2884 domain-containing protein -
  KGP22_RS18510 (KGP22_18415) - 3913906..3914232 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35623.62 Da        Isoelectric Point: 6.6965

>NTDB_id=563713 KGP22_RS18475 WP_149367656.1 3908647..3909627(-) (pilT) [Enterobacter sp. JBIWA005]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPPPDVRALLKAWLNDEQQGTWWANGQVDFAATVTGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLATLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQDGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGLQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=563713 KGP22_RS18475 WP_149367656.1 3908647..3909627(-) (pilT) [Enterobacter sp. JBIWA005]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCTCCTCCTGACGTCAGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGAACATGGTGGGCAAATGGACAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGTCTGCGCGGG
AGTGCCTTTAAGCAGATGCGCGGTGTCTCTGTGACGCTGCGTCTGTTGCCGCGTACCTGTCCACAACTCGCTACGCTGGG
CGCGCCGCGGGCAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACCGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTTGATTTTCTCAACCACCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATTGGCCAGCACAGTCCGTCATTTGCCGACGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACCG
CGGCGGAAACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTCCGGGCAGTGCTGGCGCAGAAGCTGCTTCC
CGATCTGCAGGACGGGCGCGTCGCGCTGTATGAACTGCTGGTGAACACCGCTGCGGCGGCAAATCTTATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATCCAAACCGGTCTGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.582

99.08

0.442
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365