Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KFZ68_RS02285 Genome accession   NZ_CP074084
Coordinates   451866..452900 (+) Length   344 a.a.
NCBI ID   WP_284647954.1    Uniprot ID   -
Organism   Photobacterium damselae strain Pdd1411     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446866..457900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFZ68_RS02250 (KFZ68_02255) - 447109..447513 (+) 405 WP_050787742.1 DUF2884 family protein -
  KFZ68_RS02255 (KFZ68_02260) hemW 447588..448748 (-) 1161 WP_284647952.1 radical SAM family heme chaperone HemW -
  KFZ68_RS02260 (KFZ68_02265) - 448748..449341 (-) 594 WP_044174415.1 XTP/dITP diphosphatase -
  KFZ68_RS02265 (KFZ68_02270) yggU 449443..449742 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  KFZ68_RS02270 (KFZ68_02275) - 449739..450296 (-) 558 WP_005297682.1 YggT family protein -
  KFZ68_RS02275 (KFZ68_02280) proC 450308..451126 (-) 819 WP_284647953.1 pyrroline-5-carboxylate reductase -
  KFZ68_RS02280 (KFZ68_02285) - 451142..451843 (-) 702 WP_005297686.1 YggS family pyridoxal phosphate-dependent enzyme -
  KFZ68_RS02285 (KFZ68_02290) pilT 451866..452900 (+) 1035 WP_284647954.1 type IV pilus twitching motility protein PilT Machinery gene
  KFZ68_RS02290 (KFZ68_02295) pilU 452912..454018 (+) 1107 WP_005297691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KFZ68_RS02295 (KFZ68_02300) ruvX 454346..454771 (-) 426 WP_284647955.1 Holliday junction resolvase RuvX -
  KFZ68_RS02300 (KFZ68_02305) - 454768..455331 (-) 564 WP_044174430.1 YqgE/AlgH family protein -
  KFZ68_RS02305 (KFZ68_02310) gshB 455485..456432 (-) 948 WP_005297697.1 glutathione synthase -
  KFZ68_RS02310 (KFZ68_02315) rsmE 456453..457184 (-) 732 WP_044174434.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38166.70 Da        Isoelectric Point: 6.1433

>NTDB_id=563092 KFZ68_RS02285 WP_284647954.1 451866..452900(+) (pilT) [Photobacterium damselae strain Pdd1411]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPALEHSEVHRLIFDIMNDAQRRQFEEELEVDFSFELPDVGR
FRVNAFHQSRGCSAVFRTIPMTIPTLESLNVPDVFYKIAQCQRGLVLVTGATGSGKSTTIAALVDYINEHTNRHILTIED
PIEFVHHSKRCLVNQREVHRDTHSFQAALRSALREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKSIDRI
IDVFPGSDKSMVRSMLSESLRAVVSQSLLKCISGGRVASHEIMMATPAIRNLIREDKIAQMYSMIQTGSAMGMQTMEQSV
KMLVAQGLVEAEEGRRIVDSAQQI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=563092 KFZ68_RS02285 WP_284647954.1 451866..452900(+) (pilT) [Photobacterium damselae strain Pdd1411]
ATGGATATCACCGAATTATTAGACTTCAGTGTAAAACATAACGCATCGGATCTGCATCTTTCAGCAGGTGTTCCACCTAT
GATCCGTGTAGATGGTGATGTACGCAAGTTAAGTTTACCTGCATTAGAGCACTCAGAGGTTCATCGTCTGATTTTTGATA
TCATGAATGATGCCCAACGTCGTCAATTTGAAGAAGAGTTAGAAGTCGACTTCTCATTTGAATTACCCGATGTTGGCCGT
TTTCGTGTTAACGCCTTTCACCAATCTCGAGGTTGTTCTGCGGTCTTTCGTACTATCCCTATGACCATTCCAACCTTGGA
ATCTCTGAATGTGCCCGATGTTTTCTACAAAATAGCACAATGTCAGCGTGGATTGGTATTAGTTACAGGGGCTACAGGTT
CTGGTAAATCCACCACCATTGCGGCATTGGTTGATTATATTAATGAGCATACCAATCGTCATATCCTCACGATTGAAGAT
CCAATTGAATTTGTGCATCACAGTAAACGTTGTTTGGTTAACCAACGAGAAGTGCATCGTGATACCCACAGTTTTCAAGC
CGCTTTGCGCTCGGCATTACGTGAAGATCCCGATGTGATTGTTGTGGGGGAATTGCGAGATCAAGAGACCATCAGTTTAG
CGTTAACTGCTGCAGAAACCGGACACTTGGTTTTAGGTACGCTACACACAAGTTCCGCAGCCAAAAGTATTGACCGTATT
ATCGATGTATTTCCTGGTAGTGATAAATCCATGGTTCGCTCCATGTTATCTGAGTCATTACGTGCGGTAGTTTCCCAAAG
TTTGTTGAAATGCATTAGCGGTGGTCGTGTGGCTTCCCATGAGATCATGATGGCAACTCCTGCGATTCGAAATTTGATCC
GCGAAGATAAAATTGCTCAGATGTATTCCATGATCCAAACCGGATCGGCCATGGGAATGCAGACAATGGAACAGAGTGTG
AAGATGCTGGTTGCCCAAGGACTAGTGGAAGCAGAAGAAGGTCGTCGTATTGTCGACAGTGCCCAACAGATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

79.58

96.802

0.77
  pilT Vibrio cholerae strain A1552

79.58

96.802

0.77
  pilT Pseudomonas aeruginosa PAK

70.448

97.384

0.686
  pilT Acinetobacter baumannii strain A118

70.149

97.384

0.683
  pilT Acinetobacter baumannii D1279779

70.149

97.384

0.683
  pilT Pseudomonas stutzeri DSM 10701

70.149

97.384

0.683
  pilT Acinetobacter nosocomialis M2

69.851

97.384

0.68
  pilT Acinetobacter baylyi ADP1

68.129

99.419

0.677
  pilT Legionella pneumophila strain Lp02

69.909

95.64

0.669
  pilT Legionella pneumophila strain ERS1305867

69.909

95.64

0.669
  pilT Neisseria meningitidis 8013

65.282

97.965

0.64
  pilT Neisseria gonorrhoeae MS11

64.985

97.965

0.637
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.709

100

0.497
  pilU Vibrio cholerae strain A1552

41.449

100

0.416
  pilU Pseudomonas stutzeri DSM 10701

40.87

100

0.41
  pilU Acinetobacter baylyi ADP1

38.82

93.605

0.363