Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KFZ67_RS02280 Genome accession   NZ_CP074080
Coordinates   452004..453038 (+) Length   344 a.a.
NCBI ID   WP_005297689.1    Uniprot ID   -
Organism   Photobacterium damselae strain Pdd0210     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 447004..458038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFZ67_RS02245 (KFZ67_02240) - 447247..447651 (+) 405 WP_050787742.1 DUF2884 family protein -
  KFZ67_RS02250 (KFZ67_02245) hemW 447726..448886 (-) 1161 WP_068968284.1 radical SAM family heme chaperone HemW -
  KFZ67_RS02255 (KFZ67_02250) - 448886..449479 (-) 594 WP_284660384.1 XTP/dITP diphosphatase -
  KFZ67_RS02260 (KFZ67_02255) yggU 449581..449880 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  KFZ67_RS02265 (KFZ67_02260) - 449877..450434 (-) 558 WP_005297682.1 YggT family protein -
  KFZ67_RS02270 (KFZ67_02265) proC 450446..451264 (-) 819 WP_284660385.1 pyrroline-5-carboxylate reductase -
  KFZ67_RS02275 (KFZ67_02270) - 451280..451981 (-) 702 WP_176284100.1 YggS family pyridoxal phosphate-dependent enzyme -
  KFZ67_RS02280 (KFZ67_02275) pilT 452004..453038 (+) 1035 WP_005297689.1 type IV pilus twitching motility protein PilT Machinery gene
  KFZ67_RS02285 (KFZ67_02280) pilU 453050..454156 (+) 1107 WP_005297691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KFZ67_RS02290 (KFZ67_02285) ruvX 454484..454909 (-) 426 WP_284660386.1 Holliday junction resolvase RuvX -
  KFZ67_RS02295 (KFZ67_02290) - 454906..455469 (-) 564 WP_284660387.1 YqgE/AlgH family protein -
  KFZ67_RS02300 (KFZ67_02295) gshB 455623..456570 (-) 948 WP_284660388.1 glutathione synthase -
  KFZ67_RS02305 (KFZ67_02300) rsmE 456591..457322 (-) 732 WP_284660389.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38180.73 Da        Isoelectric Point: 6.1433

>NTDB_id=563057 KFZ67_RS02280 WP_005297689.1 452004..453038(+) (pilT) [Photobacterium damselae strain Pdd0210]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPALEHSEVHRLIFDIMNDAQRRQFEEELEVDFSFELPDVGR
FRVNAFHQSRGCSAVFRTIPMTIPTLESLNVPDVFYKIAQCQRGLVLVTGATGSGKSTTIAALVDYINEHTNRHILTIED
PIEFVHHSKRCLINQREVHRDTHSFQAALRSALREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKSIDRI
IDVFPGSDKSMVRSMLSESLRAVVSQSLLKCISGGRVASHEIMMATPAIRNLIREDKIAQMYSMIQTGSAMGMQTMEQSV
KMLVAQGLVEAEEGRRIVDSAQQI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=563057 KFZ67_RS02280 WP_005297689.1 452004..453038(+) (pilT) [Photobacterium damselae strain Pdd0210]
ATGGATATCACCGAATTATTAGACTTCAGTGTAAAACATAACGCATCGGATCTGCATCTTTCAGCAGGTGTTCCACCTAT
GATCCGTGTAGATGGTGATGTACGCAAGTTAAGTTTACCTGCATTAGAGCACTCAGAGGTTCATCGTCTGATTTTTGATA
TCATGAATGATGCCCAACGTCGTCAATTTGAAGAAGAGTTAGAAGTCGACTTCTCATTTGAATTACCCGATGTTGGTCGT
TTTCGTGTTAACGCCTTTCACCAATCTCGAGGTTGTTCTGCGGTCTTTCGTACTATCCCTATGACCATTCCAACCTTGGA
ATCTCTGAATGTGCCCGATGTTTTCTACAAAATAGCACAATGTCAGCGTGGATTGGTATTAGTTACAGGGGCTACAGGTT
CTGGTAAATCCACCACCATTGCAGCATTGGTTGATTATATTAATGAGCACACCAATCGTCATATCCTCACGATTGAAGAT
CCAATTGAATTTGTGCATCACAGTAAACGTTGTTTGATTAACCAACGAGAAGTGCATCGTGATACCCACAGTTTTCAAGC
CGCTTTGCGCTCGGCATTACGTGAAGATCCCGATGTGATTGTTGTGGGGGAATTGCGAGATCAAGAGACCATCAGTTTAG
CGTTAACTGCTGCAGAAACCGGACACTTGGTTTTAGGTACGCTACACACAAGTTCAGCAGCCAAAAGTATTGACCGTATT
ATCGATGTATTTCCTGGTAGTGATAAATCCATGGTTCGCTCCATGTTATCTGAGTCATTACGTGCAGTGGTTTCCCAAAG
TTTGTTGAAATGCATTAGCGGTGGTCGTGTGGCTTCCCATGAGATCATGATGGCAACCCCTGCGATTCGAAATTTGATCC
GCGAAGATAAGATTGCTCAGATGTATTCCATGATCCAAACCGGATCGGCAATGGGAATGCAGACAATGGAACAGAGTGTG
AAGATGCTGGTTGCCCAAGGACTAGTGGAAGCAGAAGAAGGTCGTCGTATTGTCGACAGTGCCCAACAGATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

79.88

96.802

0.773
  pilT Vibrio cholerae strain A1552

79.88

96.802

0.773
  pilT Acinetobacter baumannii D1279779

70.448

97.384

0.686
  pilT Acinetobacter baumannii strain A118

70.448

97.384

0.686
  pilT Acinetobacter nosocomialis M2

70.149

97.384

0.683
  pilT Pseudomonas aeruginosa PAK

70.149

97.384

0.683
  pilT Pseudomonas stutzeri DSM 10701

69.851

97.384

0.68
  pilT Acinetobacter baylyi ADP1

68.421

99.419

0.68
  pilT Legionella pneumophila strain Lp02

69.605

95.64

0.666
  pilT Legionella pneumophila strain ERS1305867

69.605

95.64

0.666
  pilT Neisseria meningitidis 8013

65.579

97.965

0.642
  pilT Neisseria gonorrhoeae MS11

65.282

97.965

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

100

0.5
  pilU Vibrio cholerae strain A1552

41.159

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

39.13

93.605

0.366