Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   UWK_RS07295 Genome accession   NC_020304
Coordinates   1654714..1655766 (-) Length   350 a.a.
NCBI ID   WP_015403718.1    Uniprot ID   M1P8Q9
Organism   Desulfocapsa sulfexigens DSM 10523     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1649714..1660766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UWK_RS07270 (UWK_01463) - 1649803..1650597 (-) 795 WP_153304852.1 peptidase MA family metallohydrolase -
  UWK_RS07280 (UWK_01464) - 1651390..1652406 (+) 1017 WP_015403715.1 aldo/keto reductase -
  UWK_RS07285 (UWK_01465) - 1652425..1653657 (+) 1233 WP_015403716.1 MFS transporter -
  UWK_RS07290 (UWK_01466) - 1653677..1654576 (-) 900 WP_015403717.1 hypothetical protein -
  UWK_RS07295 (UWK_01467) pilT 1654714..1655766 (-) 1053 WP_015403718.1 type IV pilus twitching motility protein PilT Machinery gene
  UWK_RS07300 (UWK_01468) - 1655839..1656933 (-) 1095 WP_015403719.1 type IV pilus twitching motility protein PilT -
  UWK_RS07305 (UWK_01469) - 1656963..1657199 (-) 237 WP_015403720.1 hypothetical protein -
  UWK_RS07310 (UWK_01470) - 1657343..1659601 (+) 2259 WP_015403721.1 peptidase U32 family protein -
  UWK_RS07315 (UWK_01471) - 1659613..1660320 (-) 708 WP_015403722.1 TIGR04211 family SH3 domain-containing protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38692.70 Da        Isoelectric Point: 6.7678

>NTDB_id=56239 UWK_RS07295 WP_015403718.1 1654714..1655766(-) (pilT) [Desulfocapsa sulfexigens DSM 10523]
MAQIDTFFKQMKKHGASDLHMVVGFPPLLRLRGDLIPLDAPPLTAESAKDILFEVLDPGQQETFNKNRDFDKAYELEGVG
RFRCNFLYQHRGPGAVFRIIPTKILTLDQLGMPDVVKKIASYERGLVLVTGPTGSGKSTTMAAIIDLINDTYPKHIITIE
DPLEFVHPNKKCIFSQREIGTHAETFGTALMVANREDPDVILVGEMRDLETISLALTCAELGILVFGTLHTNSAAKTIDR
IINAFPAEQQAQTRTMLSESLKAVIAQQLLKTRDGKGRCAAIEVLIGSSALASIIREGKITQIDSLIQTGKSLGMQTMDS
HLRELVDTKQISREAAREKAINKNLFNEHE

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=56239 UWK_RS07295 WP_015403718.1 1654714..1655766(-) (pilT) [Desulfocapsa sulfexigens DSM 10523]
ATGGCTCAGATAGATACTTTTTTCAAACAGATGAAGAAACATGGTGCAAGTGACTTGCATATGGTGGTCGGTTTTCCACC
TCTGCTGCGTCTTCGCGGAGACCTTATCCCTCTCGATGCCCCTCCCCTCACAGCAGAATCCGCCAAGGATATCCTCTTTG
AAGTACTTGATCCAGGACAACAGGAAACCTTTAACAAAAACAGAGATTTTGATAAGGCCTATGAACTTGAGGGTGTGGGA
CGATTTCGTTGTAATTTTTTATATCAGCATCGAGGTCCCGGCGCAGTCTTTCGGATTATTCCCACCAAAATTCTCACTCT
CGATCAGCTCGGCATGCCAGATGTAGTCAAAAAAATAGCCTCTTACGAGAGAGGCCTGGTTCTGGTAACCGGACCTACCG
GCAGCGGAAAGTCAACAACCATGGCGGCAATTATCGATTTGATTAACGATACCTATCCAAAGCATATCATCACCATAGAA
GACCCCCTGGAGTTTGTCCATCCCAACAAAAAATGTATCTTTTCCCAAAGAGAGATCGGTACCCATGCAGAGACCTTCGG
CACGGCTTTGATGGTCGCAAACAGAGAAGATCCCGATGTCATTCTGGTGGGCGAAATGCGAGATCTTGAGACCATATCCC
TGGCCCTGACCTGTGCCGAACTCGGTATCCTGGTTTTTGGGACACTGCACACCAACAGTGCGGCAAAAACGATTGATAGA
ATCATCAATGCCTTTCCCGCAGAGCAACAGGCTCAGACCAGAACCATGCTTTCTGAGTCACTCAAGGCAGTTATTGCTCA
GCAGTTATTAAAAACCCGCGATGGAAAGGGAAGGTGCGCCGCAATTGAAGTCTTAATCGGCTCATCAGCTCTGGCATCCA
TTATCAGAGAAGGAAAAATCACCCAGATAGACTCACTGATTCAGACAGGTAAATCCCTGGGAATGCAGACCATGGACAGC
CACCTGCGGGAACTGGTGGACACCAAACAAATATCCCGGGAAGCAGCAAGAGAGAAAGCTATAAATAAAAACCTGTTCAA
CGAGCATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M1P8Q9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.488

93.714

0.52
  pilT Neisseria gonorrhoeae MS11

53.235

97.143

0.517
  pilT Pseudomonas stutzeri DSM 10701

54.878

93.714

0.514
  pilT Neisseria meningitidis 8013

52.941

97.143

0.514
  pilT Legionella pneumophila strain Lp02

52.994

95.429

0.506
  pilT Legionella pneumophila strain ERS1305867

52.994

95.429

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.459

89.714

0.489
  pilT Vibrio cholerae strain A1552

54.459

89.714

0.489
  pilT Acinetobacter nosocomialis M2

52.134

93.714

0.489
  pilT Acinetobacter baumannii D1279779

52.134

93.714

0.489
  pilT Acinetobacter baumannii strain A118

52.134

93.714

0.489
  pilT Acinetobacter baylyi ADP1

51.057

94.571

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.494

94.857

0.46
  pilU Pseudomonas stutzeri DSM 10701

41.813

97.714

0.409
  pilU Vibrio cholerae strain A1552

40.936

97.714

0.4
  pilU Acinetobacter baylyi ADP1

40.896

95.714

0.391

Multiple sequence alignment