Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KFB96_RS16700 Genome accession   NZ_CP073760
Coordinates   3669916..3670971 (+) Length   351 a.a.
NCBI ID   WP_213457260.1    Uniprot ID   -
Organism   MAG: Thiocapsa sp. isolate M50B4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3664916..3675971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFB96_RS16690 (KFB96_16690) - 3667110..3668576 (+) 1467 WP_213457264.1 glutamate synthase subunit beta -
  KFB96_RS16695 (KFB96_16695) pilU 3668670..3669908 (+) 1239 WP_213457262.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KFB96_RS16700 (KFB96_16700) pilU 3669916..3670971 (+) 1056 WP_213457260.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KFB96_RS16705 (KFB96_16705) gspD 3671003..3673186 (-) 2184 WP_300970415.1 type II secretion system secretin GspD -
  KFB96_RS16710 (KFB96_16710) - 3673230..3673823 (-) 594 WP_300970416.1 hypothetical protein -
  KFB96_RS16715 (KFB96_16715) gspM 3673820..3674434 (-) 615 WP_213457256.1 type II secretion system protein GspM -
  KFB96_RS16720 (KFB96_16720) - 3674431..3675528 (-) 1098 WP_213457254.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38884.62 Da        Isoelectric Point: 6.1903

>NTDB_id=560548 KFB96_RS16700 WP_213457260.1 3669916..3670971(+) (pilU) [MAG: Thiocapsa sp. isolate M50B4]
MDINPYLEMMIRHKASDLFFVPGAPAKIKIEGVIRSIGETPLTATFCREAIASLLDADQERELAESLELDFAVALEDRGR
FRVNAYHQRGHRAMVLRYIRSTIPTPEELDLPPVLSQLVMHKRGIILMVGATGSGKSTTLAAMIGHRNCTAPGHIITIED
PIEFVHPHQQCIVSQRELGLDTKDYRRALKSALREAPDVILIGEIRTRETMEAAIELAGTGHLAISTLHANNAYQAMERI
VNLFPHEMHKTLYTDLSAYMRAIVSQRLVRTTTGARCAAIEILVNTPHIADLIRDGHIESIEEAMQGHGEEGTTTFDEAL
LKLYHKGSITLDEALTNADSAPNLEAKINFG

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=560548 KFB96_RS16700 WP_213457260.1 3669916..3670971(+) (pilU) [MAG: Thiocapsa sp. isolate M50B4]
ATGGACATCAACCCTTATCTCGAGATGATGATTCGACACAAGGCGTCGGATCTCTTTTTCGTGCCCGGAGCACCGGCCAA
GATCAAGATCGAAGGGGTGATCCGCTCGATCGGCGAGACCCCTTTGACGGCGACCTTTTGTCGGGAGGCGATTGCTTCCC
TGCTCGATGCGGACCAGGAGCGCGAGCTCGCGGAGTCGCTCGAGCTCGATTTCGCCGTCGCGTTGGAGGATCGTGGACGC
TTTCGGGTCAACGCCTATCATCAGCGCGGACACCGGGCGATGGTCCTGCGCTACATCCGAAGCACCATCCCGACCCCCGA
GGAGTTGGATCTACCCCCGGTGTTGAGCCAATTGGTCATGCACAAGCGGGGGATCATCCTCATGGTCGGCGCGACGGGCT
CCGGCAAGTCCACGACACTCGCCGCGATGATCGGCCATCGCAATTGCACGGCGCCGGGTCACATCATCACCATCGAGGAC
CCGATCGAGTTCGTGCATCCCCACCAGCAATGCATCGTCAGTCAACGCGAGCTCGGGCTGGATACCAAGGACTACCGGCG
GGCGCTGAAGAGTGCACTGCGCGAGGCGCCCGATGTCATCCTGATCGGCGAGATCCGGACCCGCGAAACCATGGAGGCGG
CGATCGAGCTGGCCGGGACGGGTCATTTGGCCATCTCGACCCTGCACGCCAACAATGCCTATCAGGCGATGGAGCGGATC
GTCAACTTGTTTCCGCATGAGATGCACAAGACGCTCTACACCGACCTCTCGGCCTATATGCGCGCCATCGTCTCGCAACG
CCTGGTCCGCACCACCACCGGTGCGCGCTGTGCGGCCATCGAGATCCTAGTCAACACGCCGCACATCGCAGATCTCATTC
GAGACGGGCACATCGAGTCGATCGAGGAGGCGATGCAGGGACACGGCGAGGAGGGGACAACCACCTTCGACGAGGCGCTT
TTGAAGCTCTACCACAAGGGATCCATCACCTTGGATGAGGCACTCACCAACGCGGACTCGGCCCCCAACCTCGAGGCCAA
GATCAACTTCGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

49.716

100

0.499
  pilU Acinetobacter baylyi ADP1

48.725

100

0.49
  pilU Vibrio cholerae strain A1552

49.275

98.291

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

96.581

0.41
  pilT Pseudomonas aeruginosa PAK

40.116

98.006

0.393
  pilT Pseudomonas stutzeri DSM 10701

39.535

98.006

0.387
  pilT Acinetobacter baylyi ADP1

39.706

96.866

0.385
  pilT Neisseria meningitidis 8013

39.13

98.291

0.385
  pilT Neisseria gonorrhoeae MS11

39.13

98.291

0.385
  pilT Legionella pneumophila strain ERS1305867

40.606

94.017

0.382
  pilT Legionella pneumophila strain Lp02

40.606

94.017

0.382
  pilT Vibrio cholerae strain A1552

40.24

94.872

0.382
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.24

94.872

0.382
  pilT Acinetobacter baumannii strain A118

39.053

96.296

0.376
  pilT Acinetobacter baumannii D1279779

39.053

96.296

0.376
  pilT Acinetobacter nosocomialis M2

39.053

96.296

0.376