Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KD050_RS02810 Genome accession   NZ_CP073315
Coordinates   542975..544015 (-) Length   346 a.a.
NCBI ID   WP_211894754.1    Uniprot ID   -
Organism   Psychrobacillus sp. INOP01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 537975..549015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KD050_RS02785 (KD050_02785) - 538479..539489 (-) 1011 WP_211894749.1 LysM peptidoglycan-binding domain-containing protein -
  KD050_RS02790 (KD050_02790) - 539486..540088 (-) 603 WP_211894750.1 PilN domain-containing protein -
  KD050_RS02795 (KD050_02795) pilM 540100..541071 (-) 972 WP_211894751.1 type IV pilus biogenesis protein PilM -
  KD050_RS02800 (KD050_02800) - 541162..541590 (-) 429 WP_211894752.1 competence type IV pilus major pilin ComGC -
  KD050_RS02805 (KD050_02805) - 541767..542975 (-) 1209 WP_211894753.1 type II secretion system F family protein -
  KD050_RS02810 (KD050_02810) pilT 542975..544015 (-) 1041 WP_211894754.1 type IV pilus twitching motility protein PilT Machinery gene
  KD050_RS02815 (KD050_02815) - 544034..545671 (-) 1638 WP_211894755.1 GspE/PulE family protein -
  KD050_RS02820 (KD050_02820) - 545782..546210 (+) 429 WP_211894756.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KD050_RS02825 (KD050_02825) - 546234..546644 (+) 411 WP_211894757.1 type II secretion system protein J -
  KD050_RS02830 (KD050_02830) - 546660..548195 (+) 1536 WP_211894758.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38682.91 Da        Isoelectric Point: 7.0734

>NTDB_id=558992 KD050_RS02810 WP_211894754.1 542975..544015(-) (pilT) [Psychrobacillus sp. INOP01]
MKEKLHQLLQASFELGTSDLHLTVGMPPMMRLHGELKRFGQDILKPIDTEEMAKVAIVPNLWDTFIEKGDLDFSYSLPGV
SRFRVNAYYQRNCVSLAFRVIPKVIPTFEDLNLPTILHDIAKKPQGLVLVTGPTGSGKSTTLASMIQEMNQTVKKHIITL
EDPIEYLHSHGTCIINQREVGFDTKTFASGLRASLRQDPDVILVGEMRDLETISTAITAAETGHLVLATLHTTSASMTID
RIIDVFPPNQQPQVRIQLASVLQAVISQRLFQSITGKGRVAAFEILLNVPAVKNLIRNEKIHQIHSVMQSSKSMGMQTME
SELERLAKEKLVSNEQLEPYFAEKMV

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=558992 KD050_RS02810 WP_211894754.1 542975..544015(-) (pilT) [Psychrobacillus sp. INOP01]
ATGAAAGAAAAGCTACATCAGTTGTTACAAGCCTCTTTTGAACTTGGAACCTCTGATTTACATTTAACAGTTGGTATGCC
CCCAATGATGCGATTACATGGTGAATTAAAAAGATTTGGACAAGATATATTAAAACCAATAGACACGGAAGAAATGGCCA
AAGTAGCTATAGTTCCGAATCTATGGGACACATTTATAGAAAAGGGTGATCTCGATTTCTCATATAGTTTACCTGGAGTT
TCTCGATTTCGTGTAAATGCGTACTATCAACGAAATTGTGTGAGTCTTGCCTTCCGTGTAATACCAAAAGTAATTCCAAC
TTTCGAGGATTTAAATTTGCCAACTATCTTACATGATATTGCAAAGAAACCTCAAGGACTAGTATTAGTAACAGGTCCGA
CTGGTTCAGGGAAATCTACCACACTTGCATCGATGATTCAAGAAATGAATCAAACTGTCAAAAAACATATTATTACATTA
GAAGATCCAATCGAGTATTTACATTCTCATGGCACCTGTATTATCAATCAACGAGAAGTGGGTTTCGATACAAAAACTTT
TGCATCTGGATTAAGAGCTTCGCTACGACAAGATCCTGACGTTATACTAGTTGGTGAAATGAGAGATTTAGAAACAATTT
CTACAGCAATTACTGCAGCAGAAACTGGCCATCTAGTACTTGCTACATTACATACAACGAGTGCTTCTATGACGATTGAT
CGAATCATCGATGTTTTTCCGCCCAATCAGCAGCCACAGGTAAGAATTCAATTGGCTTCCGTACTTCAAGCGGTTATTTC
ACAACGACTTTTCCAATCCATTACTGGTAAAGGTAGAGTAGCTGCATTTGAGATACTTTTAAATGTTCCGGCAGTAAAAA
ATCTAATAAGAAATGAAAAAATCCATCAAATTCATTCCGTTATGCAGTCAAGCAAATCGATGGGTATGCAAACAATGGAA
AGTGAACTAGAACGTCTAGCTAAAGAGAAATTAGTATCAAACGAACAACTAGAACCCTATTTTGCGGAGAAGATGGTATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.672

95.087

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.602

95.954

0.486
  pilT Vibrio cholerae strain A1552

50.602

95.954

0.486
  pilT Pseudomonas stutzeri DSM 10701

49.701

96.532

0.48
  pilT Acinetobacter baumannii strain A118

50.459

94.509

0.477
  pilT Acinetobacter nosocomialis M2

50.459

94.509

0.477
  pilT Acinetobacter baumannii D1279779

50.459

94.509

0.477
  pilT Pseudomonas aeruginosa PAK

48.955

96.821

0.474
  pilT Acinetobacter baylyi ADP1

48.624

94.509

0.46
  pilT Neisseria meningitidis 8013

49.686

91.908

0.457
  pilT Legionella pneumophila strain Lp02

47.734

95.665

0.457
  pilT Legionella pneumophila strain ERS1305867

47.734

95.665

0.457
  pilT Neisseria gonorrhoeae MS11

49.371

91.908

0.454
  pilU Pseudomonas stutzeri DSM 10701

43.344

93.353

0.405
  pilU Vibrio cholerae strain A1552

40.964

95.954

0.393
  pilU Acinetobacter baylyi ADP1

41.195

91.908

0.379