Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H045_RS19255 Genome accession   NC_020209
Coordinates   4389699..4390733 (-) Length   344 a.a.
NCBI ID   WP_003234595.1    Uniprot ID   -
Organism   Pseudomonas poae RE*1-1-14     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4384699..4395733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H045_RS19225 (H045_19205) ruvX 4384848..4385285 (+) 438 WP_003234589.1 Holliday junction resolvase RuvX -
  H045_RS19230 (H045_19210) pyrR 4385360..4385866 (+) 507 WP_003234590.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  H045_RS19235 (H045_19215) - 4385891..4386895 (+) 1005 WP_003234591.1 aspartate carbamoyltransferase catalytic subunit -
  H045_RS19240 (H045_19220) - 4386892..4388163 (+) 1272 WP_015373211.1 dihydroorotase -
  H045_RS19245 (H045_19225) - 4388306..4388716 (-) 411 WP_163001024.1 TM2 domain-containing protein -
  H045_RS19250 (H045_19230) - 4388985..4389623 (+) 639 WP_032870762.1 C40 family peptidase -
  H045_RS19255 (H045_19235) pilT 4389699..4390733 (-) 1035 WP_003234595.1 type IV pilus twitching motility protein PilT Machinery gene
  H045_RS19260 (H045_19240) - 4390791..4391486 (+) 696 WP_015373212.1 YggS family pyridoxal phosphate-dependent enzyme -
  H045_RS19265 (H045_19245) proC 4391511..4392329 (+) 819 WP_015373213.1 pyrroline-5-carboxylate reductase -
  H045_RS19270 (H045_19250) - 4392355..4392942 (+) 588 WP_003234598.1 YggT family protein -
  H045_RS19275 (H045_19255) - 4393137..4394276 (+) 1140 WP_015373214.1 homoserine O-acetyltransferase -
  H045_RS19280 (H045_19260) metW 4394284..4394904 (+) 621 WP_003234603.1 methionine biosynthesis protein MetW -
  H045_RS19285 (H045_19265) - 4394926..4395360 (+) 435 WP_003234607.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37549.38 Da        Isoelectric Point: 7.2827

>NTDB_id=55805 H045_RS19255 WP_003234595.1 4389699..4390733(-) (pilT) [Pseudomonas poae RE*1-1-14]
MDITQLLTASVRRGASDLHLSAGLVPMLRVDGEVWPLDWPVLSPPQVADLLSPLLNQHQQKDFETSLETDFAFELPGVAR
FRVNVFRQARGVGAVFRTIPCEVQSLESLGLGEVFQRIARLPRGLVLVTGPTGSGKSTTLAAMIDDLNRHRRQHILTLED
PIEFIHRPNMALINQRQVHRDTHSFSTALRSALREDPDVILIGELRDLETIRLALTAAETGHLVFGTLHTTSAAKSVDRL
VDVFPAGEKAMVRSMLAESLQAVVSQVLVKRLGGGRVAAHEIMLGTPAIRNLIRENKVAQMVSAIQTGGAVGMKTLDMSL
RALVGEGLVSREEAREKATVPGDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=55805 H045_RS19255 WP_003234595.1 4389699..4390733(-) (pilT) [Pseudomonas poae RE*1-1-14]
ATGGATATCACTCAATTGCTGACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCATCTGTCGGCTGGTCTGGTGCCGAT
GTTGCGAGTTGATGGCGAGGTATGGCCACTGGATTGGCCGGTGCTTTCACCGCCGCAAGTGGCGGACTTGTTGAGCCCTT
TGCTCAATCAGCACCAGCAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCATTCGAACTGCCGGGCGTGGCGCGG
TTCCGCGTGAACGTGTTCCGCCAGGCGCGTGGCGTGGGGGCGGTGTTTCGCACTATTCCTTGCGAGGTTCAGAGCCTGGA
GAGCCTGGGGCTGGGCGAAGTCTTCCAGCGCATCGCGCGCTTGCCGCGCGGCCTGGTGCTGGTGACCGGTCCCACCGGCT
CGGGCAAGTCCACTACGTTGGCGGCGATGATCGATGACCTGAATCGGCATCGCCGCCAGCACATCCTCACGCTGGAAGAC
CCCATCGAATTTATCCACCGGCCGAACATGGCGCTGATCAATCAGCGTCAGGTGCACCGTGATACCCACAGCTTCTCGAC
GGCCCTGCGCTCGGCCCTGCGCGAAGACCCGGACGTGATTCTGATCGGCGAGTTGCGCGACCTGGAGACCATTCGCCTGG
CATTGACGGCGGCGGAAACCGGGCACCTGGTCTTCGGCACCCTGCACACCACGTCAGCGGCCAAAAGCGTGGACCGTCTG
GTGGATGTGTTCCCGGCGGGGGAAAAGGCCATGGTGCGCTCGATGTTGGCGGAATCACTGCAGGCGGTGGTGTCACAAGT
GCTGGTCAAGAGGCTCGGCGGCGGACGCGTTGCTGCCCATGAAATCATGCTGGGCACGCCGGCCATTCGTAATCTGATCC
GCGAGAACAAAGTGGCGCAGATGGTCTCGGCGATTCAGACCGGCGGAGCGGTGGGGATGAAGACGCTGGATATGAGCTTG
AGGGCGCTGGTGGGCGAGGGGCTGGTCAGCCGGGAAGAGGCGCGCGAGAAGGCGACGGTGCCTGGGGACATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.848

99.128

0.712
  pilT Pseudomonas stutzeri DSM 10701

69.795

99.128

0.692
  pilT Acinetobacter baumannii D1279779

68.328

99.128

0.677
  pilT Acinetobacter baumannii strain A118

68.328

99.128

0.677
  pilT Acinetobacter nosocomialis M2

68.328

99.128

0.677
  pilT Acinetobacter baylyi ADP1

67.449

99.128

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.095

97.965

0.628
  pilT Vibrio cholerae strain A1552

64.095

97.965

0.628
  pilT Neisseria meningitidis 8013

61.947

98.547

0.61
  pilT Legionella pneumophila strain Lp02

62.13

98.256

0.61
  pilT Legionella pneumophila strain ERS1305867

62.13

98.256

0.61
  pilT Neisseria gonorrhoeae MS11

61.652

98.547

0.608
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.585

99.419

0.503
  pilU Pseudomonas stutzeri DSM 10701

38.841

100

0.39
  pilU Vibrio cholerae strain A1552

38.841

100

0.39
  pilU Acinetobacter baylyi ADP1

37.572

100

0.378

Multiple sequence alignment