Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KBU60_RS02460 Genome accession   NZ_CP073068
Coordinates   508199..509305 (+) Length   368 a.a.
NCBI ID   WP_021454419.1    Uniprot ID   A0A7H5D0D6
Organism   Vibrio parahaemolyticus strain FB-11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 503199..514305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBU60_RS02425 (KBU60_02425) - 503269..503871 (-) 603 WP_005482459.1 XTP/dITP diphosphatase -
  KBU60_RS02430 (KBU60_02430) - 503989..504420 (-) 432 WP_005461703.1 DUF4426 domain-containing protein -
  KBU60_RS02435 (KBU60_02435) yggU 504548..504838 (-) 291 WP_005482461.1 DUF167 family protein YggU -
  KBU60_RS02440 (KBU60_02440) - 504838..505395 (-) 558 WP_025796429.1 YggT family protein -
  KBU60_RS02445 (KBU60_02445) proC 505448..506266 (-) 819 WP_005482485.1 pyrroline-5-carboxylate reductase -
  KBU60_RS02450 (KBU60_02450) - 506386..507099 (-) 714 WP_005461710.1 YggS family pyridoxal phosphate-dependent enzyme -
  KBU60_RS02455 (KBU60_02455) pilT 507128..508168 (+) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  KBU60_RS02460 (KBU60_02460) pilU 508199..509305 (+) 1107 WP_021454419.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KBU60_RS02465 (KBU60_02465) ruvX 509367..509792 (-) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  KBU60_RS02470 (KBU60_02470) - 509841..510404 (-) 564 WP_140288127.1 YqgE/AlgH family protein -
  KBU60_RS02475 (KBU60_02475) gshB 510518..511468 (-) 951 WP_005425823.1 glutathione synthase -
  KBU60_RS02480 (KBU60_02480) rsmE 511483..512214 (-) 732 WP_212569299.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KBU60_RS02485 (KBU60_02485) endA 512339..513034 (-) 696 WP_005493878.1 deoxyribonuclease I -
  KBU60_RS02490 (KBU60_02490) - 513154..513651 (-) 498 WP_029805631.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41429.68 Da        Isoelectric Point: 7.0235

>NTDB_id=557643 KBU60_RS02460 WP_021454419.1 508199..509305(+) (pilU) [Vibrio parahaemolyticus strain FB-11]
MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTEHDVAKLLDSAMDPDRRQEFRKSRESNFAIVRDCGRFR
ISAFFQRELPGAVIRRIETIIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEPFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=557643 KBU60_RS02460 WP_021454419.1 508199..509305(+) (pilU) [Vibrio parahaemolyticus strain FB-11]
ATGGATCTGAATAAATTTCTTGAAGGTATGTTGACGCTAAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAGAAACTCACCGAACATGATGTTGCAAAGTTGCTTGATAGCGCAA
TGGATCCGGATCGGCGTCAGGAGTTTCGCAAAAGCCGCGAATCAAACTTTGCTATCGTCAGAGATTGTGGTCGCTTCCGT
ATTAGTGCTTTTTTTCAGCGAGAACTGCCGGGAGCAGTCATTCGCCGTATCGAAACCATTATTCCTACTTTTGAGCAGTT
AAAATTGCCTTTAGTGCTGCAAGACTTAGCGATTGCCAAGCGTGGTTTGGTGCTGGTGGTCGGCGCTACTGGCTCGGGTA
AATCGACCACGATGGCGGCGATGACGGGCTATCGAAACAGCAACAAAACCGGTCACATCTTGACGGTCGAAGATCCGATC
GAATTTGTGCATGAGCATAAGCGTTGTATCGTGACTCAGCGTGAGGTGGGCTTGGATACCGAAAGTTATGAAGTCGCACT
GAAAAACTCGTTGCGCCAAGCGCCTGATATGATTTTGATTGGTGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACTTGTGTATGGCAACGTTGCACGCCAACAACGCCAACCAAGCGTTAGAACGTATTCTCCAT
TTGGTGCCTAAAGATCAAAAAGAGCAATTCTTGTTTGATTTGTCGATGAACTTAAAAGGCGTGATTGGTCAGCAGCTGAT
CCGTGATAAGAATGGTCAAGGGCGTCATGGTGTGTTTGAGATCTTGCTAAACAGTCCACGAGTGTCGGATTTGATCCGTC
GTGGTGATTTACACGAATTGAAGTCAACCATGGCACGATCCAATGAGTTTGGTATGCTGACGTTTGACCAATCGCTCTAC
AAGCTGGTGATGCAAGGTAAGATCAGCGAAGAGGATGCATTGCACAGTGCGGATTCAGCCAACGATCTTCGTTTAATGCT
GAAAACACAGCGTGGTGAGCCGTTCTCTACAGGCAGTCTTGCCAACGTCAAAATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H5D0D6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

57.714

95.109

0.549
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.923

88.315

0.397
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.349

92.663

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361