Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CST_RS05955 Genome accession   NC_020134
Coordinates   1369912..1370970 (+) Length   352 a.a.
NCBI ID   WP_015358938.1    Uniprot ID   L7VNB7
Organism   Thermoclostridium stercorarium subsp. stercorarium DSM 8532     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1364912..1375970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CST_RS05935 (Cst_c12570) - 1365240..1365452 (+) 213 WP_015358935.1 DUF3006 domain-containing protein -
  CST_RS05945 (Cst_c12590) - 1366034..1367416 (-) 1383 WP_015358936.1 MATE family efflux transporter -
  CST_RS05950 (Cst_c12600) - 1367744..1369900 (+) 2157 WP_015358937.1 ATPase, T2SS/T4P/T4SS family -
  CST_RS05955 (Cst_c12610) pilT 1369912..1370970 (+) 1059 WP_015358938.1 type IV pilus twitching motility protein PilT Machinery gene
  CST_RS05960 (Cst_c12620) pilM 1371001..1372098 (+) 1098 WP_015358939.1 type IV pilus assembly protein PilM -
  CST_RS05965 (Cst_c12630) - 1372111..1373328 (+) 1218 WP_015358940.1 type II secretion system F family protein -
  CST_RS05970 (Cst_c12640) - 1373435..1373989 (+) 555 WP_015358941.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CST_RS05975 (Cst_c12650) - 1374134..1374553 (+) 420 WP_015358942.1 hypothetical protein -
  CST_RS05980 (Cst_c12660) - 1374649..1375209 (+) 561 WP_015358943.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38857.80 Da        Isoelectric Point: 6.7710

>NTDB_id=55685 CST_RS05955 WP_015358938.1 1369912..1370970(+) (pilT) [Thermoclostridium stercorarium subsp. stercorarium DSM 8532]
MNINEYLQKAVEMDASDLHICAGLPVVIRKHGSLFFVDDHILTGEDCLSLVKQLLNDSQFAFLEEEGEIDFAYMLSGVGR
FRVNAFRQRGTYAIAIRVIPSRVPKISELGLPNILYSLAERQRGLVLITGPTGSGKSTTLAAMIDHINSTKRCHIVTIED
PIEYLHKHNKSVINQREIGSDTKSYSNALRAVLREDPDVILIGEMRDLETISIALTAAETGHLVLSTLHTLGAAKTVDRI
IDVFPPHQQGQVRSQLSTVLQGIVSQQLIKKADNAGRVVATEVMIVTPAIANMIRESRTYQINSSIHTGGEYGMHSMDAC
IASLYKRGLITYENACQYAMDPVAMKKLIALE

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=55685 CST_RS05955 WP_015358938.1 1369912..1370970(+) (pilT) [Thermoclostridium stercorarium subsp. stercorarium DSM 8532]
ATGAATATCAACGAATACTTGCAAAAAGCCGTAGAGATGGATGCTTCAGACCTTCATATTTGTGCAGGTTTACCGGTGGT
CATAAGAAAACACGGCTCGCTGTTTTTTGTCGATGACCACATACTTACAGGAGAAGATTGCCTGAGTTTGGTGAAGCAGT
TGTTGAACGACTCGCAGTTCGCGTTTCTTGAAGAAGAAGGAGAAATTGACTTTGCTTATATGCTCTCCGGTGTCGGAAGA
TTCAGGGTCAATGCTTTCAGGCAAAGAGGAACTTATGCCATAGCCATAAGGGTTATTCCGTCGAGAGTACCGAAAATCAG
TGAATTGGGGCTTCCGAATATCCTTTATTCACTGGCGGAAAGGCAGAGAGGCCTTGTCCTTATAACAGGGCCTACCGGAA
GCGGTAAATCCACAACCCTTGCCGCAATGATAGATCATATTAACAGTACAAAAAGATGCCACATTGTAACCATTGAAGAT
CCTATCGAATATTTGCATAAACACAACAAAAGCGTGATAAATCAGCGTGAAATAGGCTCGGATACTAAAAGTTACAGCAA
CGCATTGAGAGCAGTGTTAAGAGAAGATCCCGACGTTATTCTGATTGGTGAGATGAGAGATTTGGAGACAATTTCCATTG
CGTTAACCGCTGCTGAAACGGGACATCTGGTTCTGTCAACACTGCATACATTGGGAGCGGCCAAGACTGTTGACAGAATT
ATAGACGTATTCCCTCCGCATCAGCAAGGGCAGGTACGCAGCCAGCTGTCGACGGTGCTACAGGGGATTGTTTCGCAGCA
GTTGATAAAGAAAGCTGATAATGCGGGAAGAGTGGTCGCTACAGAGGTCATGATAGTAACTCCTGCGATAGCAAACATGA
TAAGGGAGTCGAGGACGTATCAGATCAACAGCAGCATTCATACGGGCGGAGAATACGGCATGCATTCCATGGATGCGTGC
ATAGCTTCTTTATATAAACGCGGTTTGATTACTTATGAAAATGCATGCCAGTATGCCATGGACCCTGTGGCAATGAAAAA
GTTAATAGCGCTGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L7VNB7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.77

97.443

0.514
  pilT Pseudomonas stutzeri DSM 10701

51.312

97.443

0.5
  pilT Neisseria meningitidis 8013

50.867

98.295

0.5
  pilT Neisseria gonorrhoeae MS11

50.578

98.295

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

98.864

0.494
  pilT Acinetobacter nosocomialis M2

50

97.159

0.486
  pilT Acinetobacter baumannii D1279779

50

97.159

0.486
  pilT Acinetobacter baumannii strain A118

50

97.159

0.486
  pilT Acinetobacter baylyi ADP1

49.415

97.159

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.057

94.034

0.48
  pilT Vibrio cholerae strain A1552

51.057

94.034

0.48
  pilT Legionella pneumophila strain ERS1305867

49.267

96.875

0.477
  pilT Legionella pneumophila strain Lp02

49.267

96.875

0.477
  pilU Pseudomonas stutzeri DSM 10701

41.311

99.716

0.412
  pilU Acinetobacter baylyi ADP1

39.773

100

0.398
  pilU Vibrio cholerae strain A1552

39.528

96.307

0.381

Multiple sequence alignment