Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J9870_RS27550 Genome accession   NZ_CP072913
Coordinates   6308748..6309782 (-) Length   344 a.a.
NCBI ID   WP_210641777.1    Uniprot ID   -
Organism   Pseudomonas sp. Tri1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6303748..6314782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9870_RS27520 ruvX 6303858..6304295 (+) 438 WP_109752386.1 Holliday junction resolvase RuvX -
  J9870_RS27525 pyrR 6304381..6304887 (+) 507 WP_210641770.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  J9870_RS27530 - 6304912..6305916 (+) 1005 WP_003177495.1 aspartate carbamoyltransferase catalytic subunit -
  J9870_RS27535 - 6305913..6307184 (+) 1272 WP_210641771.1 dihydroorotase -
  J9870_RS27540 - 6307307..6307735 (-) 429 WP_210641773.1 TM2 domain-containing protein -
  J9870_RS27545 - 6307986..6308597 (+) 612 WP_210641775.1 C40 family peptidase -
  J9870_RS27550 pilT 6308748..6309782 (-) 1035 WP_210641777.1 type IV pilus twitching motility protein PilT Machinery gene
  J9870_RS27555 - 6309836..6310516 (+) 681 WP_210641780.1 YggS family pyridoxal phosphate-dependent enzyme -
  J9870_RS27560 proC 6310553..6311371 (+) 819 WP_210641782.1 pyrroline-5-carboxylate reductase -
  J9870_RS27565 - 6311382..6311972 (+) 591 WP_210641784.1 YggT family protein -
  J9870_RS27570 - 6311978..6312271 (+) 294 WP_210641786.1 DUF167 domain-containing protein -
  J9870_RS27575 - 6312463..6313602 (+) 1140 WP_210641788.1 homoserine O-acetyltransferase -
  J9870_RS27580 metW 6313610..6314230 (+) 621 WP_210641791.1 methionine biosynthesis protein MetW -
  J9870_RS27585 - 6314257..6314691 (+) 435 WP_210641793.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38168.77 Da        Isoelectric Point: 7.0158

>NTDB_id=556829 J9870_RS27550 WP_210641777.1 6308748..6309782(-) (pilT) [Pseudomonas sp. Tri1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKVTEAPRGLVLVTGPTGSGKSTTLAAMIDHLNNHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQSFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KDLVSKGLISREHARERARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=556829 J9870_RS27550 WP_210641777.1 6308748..6309782(-) (pilT) [Pseudomonas sp. Tri1]
ATGGATATCACTGAGTTACTGGCGTTCAGCGCCAAGCAGGGCGCGTCGGACCTGCACCTGTCCGCCGGGCTGCCGCCGAT
GATCCGCGTCGATGGCGATGTGCGACGTATCAACCTGCCGGCGCTGGACCACAAGCAGGTTCATGAGCTGATCTACGACA
TCATGAACGACCGGCAACGGGTGGATTACGAGAAGTTTCTGGAAACCGATTTTTCCTTTGATGTTCCAGGTGTGGCGCGG
TTTCGGGTCAACGCCTTCAATCAGAATCGCGGTGCGGGGGCGGTGTTTCGCACCATCCCGTCGAAAGTCCTGACCATGGA
AGACCTGGGGATGGGCGAAGTGTTTCGCAAAGTGACCGAAGCGCCGCGCGGGCTGGTGTTGGTGACCGGGCCGACCGGTT
CAGGCAAGTCCACCACCCTGGCGGCGATGATCGACCACTTGAACAACCACAAGCACCATCACATCCTCACCATCGAAGAC
CCCATCGAATTCGTCCACGAACCGCGCAAATGCCTGATCAACCAGCGCGAAGTACACCGCGACACCCAAAGCTTTTCCAC
GGCCCTGCGCTCGGCTCTGCGGGAAGACCCGGACGTGATCCTGGTGGGGGAGATGCGCGACCTGGAGACCATCCGCCTGG
CCCTGACCGCCGCTGAAACCGGGCACCTGGTGTTCGGCACCTTGCACACCACGTCGGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCGGGGGACGAGAAGTCCATGGTTCGCTCGATGTTGTCGGAATCGTTGCAGGCGGTGATTTCCCAGAC
GCTGGTCAAGAAAATCGGCGGTGGCCGGATCGCCGCTCATGAAATCATGCTGGGGACCTCGGCGATCCGGAACCTGATCC
GCGAAGACAAAGTGGCGCAGATGTACTCGTCGATCCAGACCGGCGGTTCGCTGGGGATGCAGACGCTGGACATGTGCTTG
AAGGATTTGGTGAGCAAGGGCTTGATCAGCCGCGAACATGCCCGGGAGCGGGCGCGTACGCCGGATAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.372

100

0.884
  pilT Pseudomonas aeruginosa PAK

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

78.779

100

0.788
  pilT Acinetobacter baumannii strain A118

78.779

100

0.788
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

72.189

98.256

0.709
  pilT Legionella pneumophila strain ERS1305867

72.189

98.256

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

65.507

100

0.657
  pilT Neisseria gonorrhoeae MS11

65.217

100

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

39.71

100

0.398