Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MYSTI_RS31685 Genome accession   NC_020126
Coordinates   8248376..8249488 (-) Length   370 a.a.
NCBI ID   WP_015351907.1    Uniprot ID   L7UI04
Organism   Myxococcus stipitatus DSM 14675     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 8243376..8254488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYSTI_RS31670 (MYSTI_06377) - 8244029..8245417 (-) 1389 WP_015351904.1 sigma-54-dependent transcriptional regulator -
  MYSTI_RS31675 (MYSTI_06378) - 8245465..8247018 (-) 1554 WP_015351905.1 two-component system sensor histidine kinase NtrB -
  MYSTI_RS31680 (MYSTI_06379) - 8247065..8248318 (-) 1254 WP_015351906.1 type II secretion system F family protein -
  MYSTI_RS31685 (MYSTI_06380) pilT 8248376..8249488 (-) 1113 WP_015351907.1 type IV pilus twitching motility protein PilT Machinery gene
  MYSTI_RS31690 (MYSTI_06381) pilB 8249527..8251227 (-) 1701 WP_015351908.1 type IV-A pilus assembly ATPase PilB -
  MYSTI_RS31695 (MYSTI_06382) - 8251443..8252372 (-) 930 WP_015351909.1 bifunctional riboflavin kinase/FAD synthetase -
  MYSTI_RS31700 (MYSTI_06383) trmB 8252442..8253062 (+) 621 WP_015351910.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  MYSTI_RS31705 (MYSTI_06384) - 8253171..8254214 (+) 1044 WP_015351911.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40504.80 Da        Isoelectric Point: 8.2933

>NTDB_id=55660 MYSTI_RS31685 WP_015351907.1 8248376..8249488(-) (pilT) [Myxococcus stipitatus DSM 14675]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEDNELDLSFGVKGLS
RFRANIFMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQLSFVLEGVMSQSLVAKAGAPGRVLALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKFGMQTFN
QALAALLLRRVISQDEAFGRSSDPDELRNILATGGGMPVGQRAPGGPAGR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=55660 MYSTI_RS31685 WP_015351907.1 8248376..8249488(-) (pilT) [Myxococcus stipitatus DSM 14675]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACGTCACCACCGGCTCTCCGCC
CCAGTTGCGCGTGGACGGCGAGCTCGTCCCCTTGAAGACGGCGCCGCTGACGCCCGTGGAGACCAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAGGAGGACAACGAGCTGGACCTGTCCTTCGGCGTGAAGGGCCTGTCG
CGCTTCCGCGCCAACATCTTCATGCAGCGTGGCGCGGTGGCCGGGGCCTTCCGCACCATTCCCTTCAAGATCCTGACCTT
CCAGGAGCTGGGCCTGCCGCCCGTGGTCGCGGAGCTGGTGAAGAAGCCGCGCGGCCTCATCCTGGTGACGGGCCCCACGG
GCTCGGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCACACAAGAACTGCCTGGTGAACCAGCGCGAGGTGGGGGCGGACACCCGGAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACGTGGTGCTGGTCGGTGAGCTCCGAGACCTGGAGACCATCGAAG
CGGCGCTCACCATCGCGGAGACAGGCCATATCTGCTACGCGACACTGCACACCAACAGCGCGGTGCAGACCATCAACCGC
GTGCTGGACGTGTTCCCGCCGTACCAGCAGCCCCAGGTGCGCGCGCAGCTCTCCTTCGTGCTGGAGGGCGTGATGAGCCA
GTCGCTGGTGGCCAAGGCGGGTGCTCCGGGCCGCGTGCTGGCCCTGGAGGTCATGGTGCCCAACCCCGCCATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGATCTACTCCTCCATGCAGGTGGGTCAGGCGAAGTTCGGCATGCAGACGTTCAAC
CAGGCGCTGGCCGCGCTGCTCCTGCGGCGGGTCATCTCCCAGGACGAGGCCTTCGGACGCTCCAGCGACCCGGACGAGCT
GCGAAACATCCTGGCCACGGGAGGTGGAATGCCGGTCGGACAGCGGGCTCCAGGCGGGCCAGCGGGTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L7UI04

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.541

100

0.505
  pilT Legionella pneumophila strain ERS1305867

53.433

90.541

0.484
  pilT Legionella pneumophila strain Lp02

53.433

90.541

0.484
  pilT Acinetobacter baumannii D1279779

51.014

93.243

0.476
  pilT Acinetobacter baumannii strain A118

51.014

93.243

0.476
  pilT Acinetobacter nosocomialis M2

51.014

93.243

0.476
  pilT Pseudomonas stutzeri DSM 10701

51.014

93.243

0.476
  pilT Pseudomonas aeruginosa PAK

51.014

93.243

0.476
  pilT Acinetobacter baylyi ADP1

50.435

93.243

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.802

90.27

0.441
  pilT Vibrio cholerae strain A1552

48.802

90.27

0.441
  pilU Vibrio cholerae strain A1552

42.577

96.486

0.411
  pilT Neisseria meningitidis 8013

44.282

92.162

0.408
  pilT Neisseria gonorrhoeae MS11

44.282

92.162

0.408
  pilU Pseudomonas stutzeri DSM 10701

40.741

94.865

0.386
  pilU Acinetobacter baylyi ADP1

40.058

93.784

0.376

Multiple sequence alignment