Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KAF59_RS11975 Genome accession   NZ_CP072849
Coordinates   2648461..2649498 (-) Length   345 a.a.
NCBI ID   WP_000350198.1    Uniprot ID   A0A0X1L2R0
Organism   Vibrio cholerae MO10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2643461..2654498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAF59_RS11950 (KAF59_11950) - 2643932..2644495 (+) 564 WP_001054768.1 YqgE/AlgH family protein -
  KAF59_RS11955 (KAF59_11955) ruvX 2644576..2644998 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  KAF59_RS11960 (KAF59_11960) tyrS 2645036..2646316 (-) 1281 WP_001088155.1 tyrosine--tRNA ligase -
  KAF59_RS11965 (KAF59_11965) - 2646425..2647222 (-) 798 WP_001123226.1 helix-turn-helix transcriptional regulator -
  KAF59_RS11970 (KAF59_11970) pilU 2647340..2648446 (-) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KAF59_RS11975 (KAF59_11975) pilT 2648461..2649498 (-) 1038 WP_000350198.1 type IV pilus twitching motility protein PilT Machinery gene
  KAF59_RS11980 (KAF59_11980) - 2649524..2650234 (+) 711 WP_001256653.1 YggS family pyridoxal phosphate-dependent enzyme -
  KAF59_RS11985 (KAF59_11985) proC 2650287..2651105 (+) 819 WP_000437941.1 pyrroline-5-carboxylate reductase -
  KAF59_RS11990 (KAF59_11990) - 2651159..2651716 (+) 558 WP_001087263.1 YggT family protein -
  KAF59_RS11995 (KAF59_11995) yggU 2651716..2652006 (+) 291 WP_000718491.1 DUF167 family protein YggU -
  KAF59_RS12000 (KAF59_12000) - 2652052..2652483 (+) 432 WP_001233674.1 DUF4426 domain-containing protein -
  KAF59_RS12005 (KAF59_12005) - 2652553..2653155 (+) 603 WP_000725019.1 XTP/dITP diphosphatase -
  KAF59_RS12010 (KAF59_12010) hemW 2653149..2654324 (+) 1176 WP_000957351.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38162.72 Da        Isoelectric Point: 6.5570

>NTDB_id=556331 KAF59_RS11975 WP_000350198.1 2648461..2649498(-) (pilT) [Vibrio cholerae MO10]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELSDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=556331 KAF59_RS11975 WP_000350198.1 2648461..2649498(-) (pilT) [Vibrio cholerae MO10]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTCATCCCGACGCTAGA
GCAACTGGATGCTCCTGAAATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGCT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCTCATCACAATAAGCATATTTTGACCATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATCCTCGTCGGTGAGCTGAGTGACCAAGAAACGATTAGCTTGG
CGCTTACTGCGGCAGAAACCGGTCACTTGGTATTTGGCACTCTGCACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGTTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGCGTCGGGGGTGGCCGTGTTGCGTGTCATGAAATCATGCTGGCGACGCCGGCAATTCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCGCGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X1L2R0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

99.71

100

0.997
  pilT Vibrio cholerae strain A1552

99.71

100

0.997
  pilT Acinetobacter baumannii D1279779

71.429

97.391

0.696
  pilT Acinetobacter baumannii strain A118

71.429

97.391

0.696
  pilT Acinetobacter nosocomialis M2

71.131

97.391

0.693
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

66.471

98.551

0.655
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

64.242

95.652

0.614
  pilT Neisseria gonorrhoeae MS11

63.939

95.652

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.682

94.783

0.49
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.533

97.971

0.397
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374