Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D781_RS18165 Genome accession   NC_020064
Coordinates   3987453..3988445 (-) Length   330 a.a.
NCBI ID   WP_015673794.1    Uniprot ID   L0MMH2
Organism   Serratia sp. FGI94     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3982453..3993445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D781_RS18150 (D781_3767) - 3982600..3983022 (-) 423 WP_233494101.1 DUF1090 domain-containing protein -
  D781_RS18155 (D781_3768) - 3983145..3986681 (-) 3537 WP_015673792.1 transporter substrate-binding domain-containing protein -
  D781_RS24320 (D781_3769) - 3986674..3987306 (-) 633 WP_015673793.1 response regulator transcription factor -
  D781_RS18165 (D781_3770) pilT 3987453..3988445 (-) 993 WP_015673794.1 type IV pilus twitching motility protein PilT Machinery gene
  D781_RS18170 (D781_3771) - 3988466..3989173 (+) 708 WP_015673795.1 YggS family pyridoxal phosphate-dependent enzyme -
  D781_RS18175 (D781_3772) proC 3989194..3990015 (+) 822 WP_015673796.1 pyrroline-5-carboxylate reductase -
  D781_RS18180 (D781_3773) - 3990035..3990589 (+) 555 WP_015673797.1 YggT family protein -
  D781_RS18185 (D781_3774) yggU 3990586..3990876 (+) 291 WP_015673798.1 DUF167 family protein YggU -
  D781_RS18190 (D781_3775) - 3990894..3991490 (+) 597 WP_015673799.1 XTP/dITP diphosphatase -
  D781_RS18195 (D781_3776) hemW 3991480..3992631 (+) 1152 WP_015673800.1 radical SAM family heme chaperone HemW -
  D781_RS23035 (D781_3777) - 3992666..3992914 (-) 249 WP_015673801.1 DUF2442 domain-containing protein -
  D781_RS23270 (D781_3778) - 3992898..3993068 (-) 171 WP_080062367.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35602.02 Da        Isoelectric Point: 7.0024

>NTDB_id=55608 D781_RS18165 WP_015673794.1 3987453..3988445(-) (pilT) [Serratia sp. FGI94]
MDIDEFVALSVKHNASDLHLCTGQPLMMRIDGELQPLAGGAVLPAGAVQRLCAALLDEPQQRQLRRAGQLDCALTTAGGT
RLRANFFYQRAGVSLALRRIAECCPTLEQLQAPAIIPALTEREEGLILVTGATGSGKSTTLAAMIDHINRHQRRHILTLE
DPIEFIHHSVLSLIQQRELGRDTCSFDAALSAALREDPDVILVGELRDSLSIRLALTAAETGHLVLATLHTRNAPQAVER
VIDVFPAEEKAYVRAQLASGLQAVIAQKLLRRAEGGRTAVFEILTATPAVSSMIREGKTHQLASVLQSGAQAGMQTFAQG
MQQRIAQGIL

Nucleotide


Download         Length: 993 bp        

>NTDB_id=55608 D781_RS18165 WP_015673794.1 3987453..3988445(-) (pilT) [Serratia sp. FGI94]
ATGGATATCGATGAATTTGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTACCGGCCAACCGTTGAT
GATGCGCATCGATGGTGAACTTCAGCCGCTGGCGGGCGGCGCCGTTCTGCCCGCCGGGGCGGTGCAGCGGCTGTGCGCGG
CGCTGCTTGACGAGCCTCAGCAGCGGCAATTACGGCGCGCCGGGCAGCTGGATTGCGCATTGACAACCGCGGGCGGCACG
CGGCTGCGCGCCAACTTTTTTTATCAGCGCGCCGGCGTGTCGCTGGCGCTGCGCCGCATTGCGGAATGCTGTCCGACGCT
GGAGCAGCTGCAGGCGCCGGCGATTATCCCGGCGCTCACCGAGCGGGAGGAGGGGCTGATTCTGGTCACGGGCGCGACCG
GTAGCGGCAAATCCACCACGCTGGCGGCGATGATCGACCATATCAACCGTCATCAGCGGCGGCATATTCTGACGCTGGAA
GATCCGATTGAGTTTATCCACCACAGCGTGCTGTCGCTGATTCAGCAGCGCGAGCTGGGGCGTGATACCTGCAGCTTCGA
CGCGGCGCTGAGTGCCGCACTGCGCGAAGATCCCGACGTGATCCTGGTCGGCGAGCTGCGCGACAGCCTCAGCATCCGGC
TGGCGCTGACCGCCGCGGAAACCGGCCACCTGGTGCTGGCGACCCTGCACACCCGCAACGCGCCGCAGGCGGTTGAGCGG
GTGATCGACGTCTTCCCGGCGGAGGAGAAAGCCTACGTGCGCGCCCAGCTGGCCAGCGGTTTGCAGGCGGTCATCGCGCA
GAAATTACTGCGCAGGGCCGAAGGCGGCAGAACGGCGGTGTTTGAGATCCTGACGGCGACGCCGGCGGTAAGCAGCATGA
TCCGTGAAGGAAAAACGCATCAGCTGGCCAGCGTGCTGCAAAGCGGCGCACAGGCGGGTATGCAAACCTTTGCTCAGGGA
ATGCAGCAGCGTATTGCGCAGGGAATTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L0MMH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

52.727

100

0.527
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.727

100

0.527
  pilT Acinetobacter baylyi ADP1

49.55

100

0.5
  pilT Acinetobacter baumannii strain A118

49.249

100

0.497
  pilT Acinetobacter baumannii D1279779

49.249

100

0.497
  pilT Acinetobacter nosocomialis M2

48.949

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.204

100

0.488
  pilT Neisseria meningitidis 8013

47.904

100

0.485
  pilT Pseudomonas stutzeri DSM 10701

47.447

100

0.479
  pilT Legionella pneumophila strain ERS1305867

47.273

100

0.473
  pilT Legionella pneumophila strain Lp02

47.273

100

0.473
  pilT Pseudomonas aeruginosa PAK

46.547

100

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.484

96.364

0.458
  pilU Vibrio cholerae strain A1552

41.358

98.182

0.406
  pilU Pseudomonas stutzeri DSM 10701

39.514

99.697

0.394
  pilU Acinetobacter baylyi ADP1

39.009

97.879

0.382

Multiple sequence alignment