Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J6320_RS21045 Genome accession   NZ_CP072782
Coordinates   2798537..2799577 (-) Length   346 a.a.
NCBI ID   WP_248487003.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain HY9901     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2793537..2804577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J6320_RS21015 (J6320_20905) endA 2793682..2794377 (+) 696 WP_213963128.1 deoxyribonuclease I -
  J6320_RS21020 (J6320_20910) rsmE 2794491..2795222 (+) 732 WP_154205024.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J6320_RS21025 (J6320_20915) gshB 2795236..2796186 (+) 951 WP_248487001.1 glutathione synthase -
  J6320_RS21030 (J6320_20920) - 2796314..2796877 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  J6320_RS21035 (J6320_20925) ruvX 2796913..2797338 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  J6320_RS21040 (J6320_20930) pilU 2797400..2798506 (-) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J6320_RS21045 (J6320_20935) pilT 2798537..2799577 (-) 1041 WP_248487003.1 type IV pilus twitching motility protein PilT Machinery gene
  J6320_RS21050 (J6320_20940) - 2799606..2800307 (+) 702 WP_154205023.1 YggS family pyridoxal phosphate-dependent enzyme -
  J6320_RS21055 (J6320_20945) proC 2800467..2801285 (+) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  J6320_RS21060 (J6320_20950) - 2801338..2801895 (+) 558 WP_005381012.1 YggT family protein -
  J6320_RS21065 (J6320_20955) yggU 2801895..2802185 (+) 291 WP_005393980.1 DUF167 family protein YggU -
  J6320_RS21070 (J6320_20960) - 2802314..2802745 (+) 432 WP_005381010.1 DUF4426 domain-containing protein -
  J6320_RS21075 (J6320_20965) - 2802864..2803466 (+) 603 WP_213963126.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38531.04 Da        Isoelectric Point: 6.6198

>NTDB_id=555638 J6320_RS21045 WP_248487003.1 2798537..2799577(-) (pilT) [Vibrio alginolyticus strain HY9901]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTINRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=555638 J6320_RS21045 WP_248487003.1 2798537..2799577(-) (pilT) [Vibrio alginolyticus strain HY9901]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCGGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTGAGAAAACTAGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAGTTGCATAACGTTGGCCGA
TTCCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTATTCCGTACCATTCCAAGTAGCATTCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTGGTCACTGGCCCAACAGGTT
CAGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCACGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATCAACCAGCGTGAAGTTCACCGTGACACCCATAGTTTCCAAAA
TGCTTTGCGTAGTGCATTGCGCGAAGACCCAGACGTTATTCTGGTTGGTGAGATGCGTGACAAAGAAACCATCAGCCTTG
CCTTAACGGCGGCAGAAACGGGTCACTTAGTTTTCGGGACGTTGCACACCAGCTCGGCGGCAAAAACCATTAACCGTATT
ATCGATGTGTTTCCTGGTAGTGATAAAGATATGGTCCGCTCGATGCTATCTGAGTCCCTTCGTTCGGTTATCGCGCAAAA
GCTTCTGAAGCGTAATGGTGGTGGCCGTATTGCATGCCATGAAATTATGATGGCAACGCCTGCGATTCGTAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATTATTCAAACAGGTGCGGCTCACGGCATGCAAACCATGGAGCAAAATGCT
CGTCAGTTAATGGCGCAAGGCATGGTTGCTCGTGAAGAGGTCGATAGTAAGATCGGATTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

71.818

95.376

0.685
  pilT Acinetobacter baumannii strain A118

71.818

95.376

0.685
  pilT Acinetobacter nosocomialis M2

71.515

95.376

0.682
  pilT Acinetobacter baylyi ADP1

71.125

95.087

0.676
  pilT Pseudomonas aeruginosa PAK

68.843

97.399

0.671
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.399

0.668
  pilT Legionella pneumophila strain Lp02

67.976

95.665

0.65
  pilT Legionella pneumophila strain ERS1305867

67.976

95.665

0.65
  pilT Neisseria meningitidis 8013

65.465

96.243

0.63
  pilT Neisseria gonorrhoeae MS11

65.165

96.243

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.516

91.908

0.483
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.532

0.39
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361