Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THETHE_RS06010 Genome accession   NC_019970
Coordinates   1236156..1237208 (+) Length   350 a.a.
NCBI ID   WP_015311521.1    Uniprot ID   L0IKZ2
Organism   Thermoanaerobacterium thermosaccharolyticum M0795     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1231156..1242208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THETHE_RS05990 (Thethe_01239) sigK 1232061..1232756 (+) 696 WP_015311517.1 RNA polymerase sporulation sigma factor SigK -
  THETHE_RS05995 (Thethe_01240) - 1232901..1233386 (+) 486 WP_041589423.1 YqeG family HAD IIIA-type phosphatase -
  THETHE_RS06000 (Thethe_01241) aroE 1233393..1234244 (+) 852 WP_015311519.1 shikimate dehydrogenase -
  THETHE_RS06005 (Thethe_01242) - 1234401..1236095 (+) 1695 WP_015311520.1 GspE/PulE family protein -
  THETHE_RS06010 (Thethe_01243) pilT 1236156..1237208 (+) 1053 WP_015311521.1 type IV pilus twitching motility protein PilT Machinery gene
  THETHE_RS06015 (Thethe_01244) - 1237238..1238452 (+) 1215 WP_015311522.1 type II secretion system F family protein -
  THETHE_RS06020 (Thethe_01245) - 1238483..1238875 (+) 393 WP_015311523.1 competence type IV pilus major pilin ComGC -
  THETHE_RS06025 (Thethe_01246) - 1238960..1239715 (+) 756 WP_015311524.1 A24 family peptidase -
  THETHE_RS06030 (Thethe_01247) - 1239755..1240219 (+) 465 WP_015311525.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THETHE_RS06035 (Thethe_01248) - 1240230..1240871 (+) 642 WP_015311526.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THETHE_RS06040 (Thethe_01249) - 1240862..1241323 (+) 462 WP_015311527.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THETHE_RS06045 (Thethe_01250) - 1241313..1241888 (+) 576 WP_013297766.1 pilus assembly PilX N-terminal domain-containing protein -
  THETHE_RS06050 (Thethe_01251) - 1241903..1242178 (+) 276 WP_015311528.1 late competence development ComFB family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38715.04 Da        Isoelectric Point: 6.8468

>NTDB_id=55537 THETHE_RS06010 WP_015311521.1 1236156..1237208(+) (pilT) [Thermoanaerobacterium thermosaccharolyticum M0795]
MKTIELLTLVVKNGASDLHITVGVPPVLRINGQLVKLDLPQLTPQDTEEITRDLLSSDELKKLEETGDIDLSYSVQGLGR
FRINAYKQRGTYSLAIRSVALRIPTIDELGLPKVIKELSLKTRGLILVTGPTGSGKSTTLASMIDLINDVRNCHILTLED
PIEYLHKHKKSIVNQREIGHDTLSYASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGSAKTIDRI
IDVFPPYQQQQIKVQLSNVLEGIISQQLLPKLDNSGRVVAVEVMIATPAIRNLIREGKSFQIQSMVQTGSKFGMMTMDMS
ISQLLKKNLISMDDALTYCVDRENFSKLAL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=55537 THETHE_RS06010 WP_015311521.1 1236156..1237208(+) (pilT) [Thermoanaerobacterium thermosaccharolyticum M0795]
ATGAAAACAATTGAACTATTGACGCTAGTTGTAAAAAACGGTGCATCTGATTTACATATAACGGTAGGTGTTCCTCCTGT
ATTAAGAATAAATGGGCAACTTGTAAAACTCGATTTGCCCCAATTGACTCCGCAAGATACGGAAGAAATCACGAGAGATT
TACTTTCAAGCGATGAACTAAAAAAACTTGAAGAGACAGGAGACATAGACCTTTCGTATTCTGTACAAGGGCTTGGAAGA
TTTAGAATAAATGCCTACAAACAAAGAGGTACATATAGTCTTGCAATAAGGTCTGTGGCATTGAGAATACCGACTATCGA
TGAATTGGGACTTCCAAAGGTCATAAAAGAGCTTTCATTGAAAACTCGTGGCCTTATACTTGTAACAGGTCCAACAGGCA
GCGGGAAATCCACAACACTTGCATCTATGATTGATCTGATAAATGATGTGCGAAATTGCCATATATTGACTCTTGAAGAT
CCGATAGAATATCTACATAAACACAAAAAAAGCATAGTAAATCAAAGAGAGATAGGTCACGATACATTATCTTATGCTAG
TGCATTGAGGGCAGCTTTAAGGGAAGATCCTGATGTTATACTTGTAGGTGAGATGAGAGATTTAGAAACAATACAGATAG
CTATAACTGCTGCTGAAACAGGACATCTCGTATTATCTACATTGCATACTATAGGCTCTGCTAAGACTATTGACAGAATC
ATAGATGTATTTCCGCCGTATCAACAACAGCAGATAAAAGTACAGCTTTCGAATGTGTTAGAAGGCATAATTTCACAGCA
GTTGCTGCCTAAACTTGATAATTCAGGACGTGTTGTGGCTGTTGAGGTAATGATAGCGACTCCTGCCATAAGGAATTTGA
TTAGAGAAGGTAAATCATTCCAAATCCAATCTATGGTTCAAACAGGAAGTAAATTTGGAATGATGACAATGGATATGTCA
ATATCACAGCTTTTAAAAAAGAATTTGATTTCGATGGATGATGCATTGACATATTGTGTAGACAGGGAAAACTTTTCAAA
ATTGGCATTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L0IKZ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.145

98.571

0.494
  pilT Legionella pneumophila strain Lp02

50.145

98.571

0.494
  pilT Legionella pneumophila strain ERS1305867

50.145

98.571

0.494
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

98.571

0.491
  pilT Acinetobacter baylyi ADP1

49.855

98.571

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.565

98.571

0.489
  pilT Acinetobacter nosocomialis M2

49.275

98.571

0.486
  pilT Acinetobacter baumannii D1279779

49.275

98.571

0.486
  pilT Acinetobacter baumannii strain A118

49.275

98.571

0.486
  pilT Neisseria meningitidis 8013

46.398

99.143

0.46
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.788

94.286

0.46
  pilT Vibrio cholerae strain A1552

48.788

94.286

0.46
  pilT Neisseria gonorrhoeae MS11

45.665

98.857

0.451
  pilU Vibrio cholerae strain A1552

40

100

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.708

96.857

0.394
  pilU Acinetobacter baylyi ADP1

40

97.143

0.389

Multiple sequence alignment