Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J8C02_RS08340 Genome accession   NZ_CP072646
Coordinates   2008837..2010006 (+) Length   389 a.a.
NCBI ID   WP_211425560.1    Uniprot ID   -
Organism   Chloracidobacterium sp. MS 40/45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2003837..2015006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8C02_RS08330 (J8C02_08330) - 2003959..2006616 (-) 2658 WP_211425558.1 ComEC/Rec2 family competence protein -
  J8C02_RS08335 (J8C02_08335) pilB 2006816..2008660 (+) 1845 WP_211425559.1 type IV-A pilus assembly ATPase PilB -
  J8C02_RS08340 (J8C02_08340) pilT 2008837..2010006 (+) 1170 WP_211425560.1 type IV pilus twitching motility protein PilT Machinery gene
  J8C02_RS08345 (J8C02_08345) - 2010275..2011498 (+) 1224 WP_058867079.1 type II secretion system F family protein -
  J8C02_RS08350 (J8C02_08350) - 2011609..2012490 (-) 882 WP_211425561.1 hypothetical protein -
  J8C02_RS08355 (J8C02_08355) - 2012610..2013251 (+) 642 WP_211421763.1 zinc ribbon domain-containing protein -
  J8C02_RS08360 (J8C02_08360) - 2013360..2013674 (-) 315 WP_211425562.1 hypothetical protein -
  J8C02_RS08365 (J8C02_08365) - 2013711..2013971 (-) 261 WP_014100186.1 TM2 domain-containing protein -
  J8C02_RS08370 (J8C02_08370) - 2014217..2014630 (-) 414 WP_246604591.1 TM2 domain-containing protein -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 43181.58 Da        Isoelectric Point: 7.9023

>NTDB_id=554789 J8C02_RS08340 WP_211425560.1 2008837..2010006(+) (pilT) [Chloracidobacterium sp. MS 40/45]
MSSNFVLSDLLRKMIELGGSDLHITTNSPPQVRVHGHLRPLDYPEMTPADTKQLAYSVLTDAQKHRFEETLELDFSFGIK
GMSRFRANLFNQRGAVGAVFRAIPYEIRTFEDLGLPPVVRKLCEKPRGLILVTGPTGSGKSTTLASMINKINEDRHEHII
TIEDPIEFLHPHKNCLVNQREVHADTHSFANSLRAALRQDPDVVLIGEMRDLETVETALRIAETGHLTFGTLHTNSAYST
INRIIDIFPSHQQSQIRTQLSLVLEGVLCQSLLPKANGQGRCMALEILVPNSAIRNLIREDKIHQIYGMMQTGQDKFGMQ
TFNQSLLSLYLSRQITLEVAMARSHNPDELQDLINRATSGVGGGAMPPGGRPMPPGGPARPGMPPPVRR

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=554789 J8C02_RS08340 WP_211425560.1 2008837..2010006(+) (pilT) [Chloracidobacterium sp. MS 40/45]
ATGTCGTCAAACTTTGTGCTGAGCGACCTGCTTCGGAAAATGATTGAACTGGGCGGCTCAGACCTCCACATCACGACGAA
CTCGCCACCCCAGGTTCGGGTTCACGGGCACCTTCGTCCGCTCGACTACCCGGAAATGACGCCGGCGGATACCAAGCAAC
TGGCCTACTCCGTACTGACCGATGCCCAAAAGCACCGTTTCGAGGAAACCCTTGAACTCGACTTCTCATTCGGTATCAAA
GGCATGTCCCGCTTCCGGGCCAACCTCTTCAACCAGCGTGGCGCGGTGGGGGCCGTCTTCCGGGCCATTCCCTACGAAAT
CCGCACCTTTGAGGACCTGGGACTGCCACCGGTAGTCAGAAAGCTGTGTGAAAAGCCGCGCGGTCTCATTCTGGTCACCG
GGCCGACGGGTTCCGGCAAGTCCACCACCCTGGCTTCGATGATCAACAAAATCAACGAAGACCGGCACGAGCACATCATC
ACCATCGAAGACCCCATCGAGTTTCTCCATCCCCACAAGAACTGTCTCGTCAACCAGCGTGAGGTCCACGCCGACACACA
CAGTTTTGCCAACTCCCTGCGTGCCGCCCTGCGTCAGGACCCTGACGTGGTGCTCATCGGGGAGATGCGCGACCTGGAAA
CCGTCGAAACGGCATTGCGTATCGCCGAAACCGGACACCTGACCTTCGGGACGCTGCACACGAACTCGGCTTACTCGACC
ATCAACCGTATCATTGACATCTTTCCGTCCCACCAGCAGTCCCAGATTCGGACGCAGCTCAGCCTCGTTCTCGAAGGTGT
GCTGTGCCAGTCCCTCCTCCCCAAAGCCAATGGACAGGGACGCTGCATGGCGCTCGAAATCCTCGTCCCGAACTCGGCAA
TCCGCAACCTCATCCGGGAAGACAAAATCCACCAGATTTACGGGATGATGCAAACCGGGCAGGACAAGTTCGGGATGCAG
ACCTTCAATCAGTCGCTGCTTAGCCTGTATCTCAGCCGTCAGATTACGCTCGAAGTGGCCATGGCGCGGTCGCACAATCC
CGATGAGTTACAGGACCTCATCAACCGTGCCACCAGTGGCGTTGGCGGCGGGGCGATGCCCCCTGGCGGGCGGCCCATGC
CACCGGGTGGCCCGGCGCGCCCCGGCATGCCCCCCCCGGTTCGCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.267

96.144

0.483
  pilT Legionella pneumophila strain Lp02

53.801

87.918

0.473
  pilT Legionella pneumophila strain ERS1305867

53.801

87.918

0.473
  pilT Acinetobacter baylyi ADP1

50.872

88.432

0.45
  pilT Pseudomonas stutzeri DSM 10701

50.872

88.432

0.45
  pilT Acinetobacter nosocomialis M2

50.581

88.432

0.447
  pilT Acinetobacter baumannii D1279779

50.581

88.432

0.447
  pilT Acinetobacter baumannii strain A118

50.581

88.432

0.447
  pilT Neisseria meningitidis 8013

50.291

88.432

0.445
  pilT Neisseria gonorrhoeae MS11

49.709

88.432

0.44
  pilT Pseudomonas aeruginosa PAK

49.419

88.432

0.437
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.411

82.005

0.422
  pilT Vibrio cholerae strain A1552

51.411

82.005

0.422
  pilU Pseudomonas stutzeri DSM 10701

44.348

88.689

0.393
  pilU Vibrio cholerae strain A1552

42.939

89.203

0.383
  pilU Acinetobacter baylyi ADP1

41.499

89.203

0.37