Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J8C00_RS09450 Genome accession   NZ_CP072644
Coordinates   2210042..2211211 (+) Length   389 a.a.
NCBI ID   WP_058867080.1    Uniprot ID   -
Organism   Chloracidobacterium sp. E     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2205042..2216211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8C00_RS09440 (J8C00_09440) - 2205165..2207903 (-) 2739 WP_211424782.1 ComEC/Rec2 family competence protein -
  J8C00_RS09445 (J8C00_09445) pilB 2208022..2209866 (+) 1845 WP_211421760.1 type IV-A pilus assembly ATPase PilB -
  J8C00_RS09450 (J8C00_09450) pilT 2210042..2211211 (+) 1170 WP_058867080.1 type IV pilus twitching motility protein PilT Machinery gene
  J8C00_RS09455 (J8C00_09455) - 2211480..2212703 (+) 1224 WP_211421761.1 type II secretion system F family protein -
  J8C00_RS09460 (J8C00_09460) - 2212835..2213716 (-) 882 WP_211421762.1 hypothetical protein -
  J8C00_RS09465 (J8C00_09465) - 2213836..2214477 (+) 642 WP_211421763.1 zinc ribbon domain-containing protein -
  J8C00_RS09470 (J8C00_09470) - 2214579..2214923 (-) 345 WP_211421764.1 hypothetical protein -
  J8C00_RS09475 (J8C00_09475) - 2214930..2215190 (-) 261 WP_014100186.1 TM2 domain-containing protein -
  J8C00_RS09480 (J8C00_09480) - 2215437..2215763 (-) 327 WP_246840684.1 TM2 domain-containing protein -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 43195.60 Da        Isoelectric Point: 7.9023

>NTDB_id=554779 J8C00_RS09450 WP_058867080.1 2210042..2211211(+) (pilT) [Chloracidobacterium sp. E]
MSSNFVLSDLLRKMIELGGSDLHITTNSPPQVRVHGHLRPLDYPEMTPADTKQLAYSVLTDAQKHRFEETLELDFSFGIK
GMSRFRANLFNQRGAVAAVFRAIPYEIRTFEDLGLPPVVRKLCEKPRGLILVTGPTGSGKSTTLASMINKINEDRHEHII
TIEDPIEFLHPHKNCLVNQREVHADTHSFANSLRAALRQDPDVVLIGEMRDLETVETALRIAETGHLTFGTLHTNSAYST
INRIIDIFPSHQQSQIRTQLSLVLEGVLCQSLLPKANGQGRCMALEILVPNSAIRNLIREDKIHQIYGMMQTGQDKFGMQ
TFNQSLLSLYLSRQITLEVAMARSHNPDELQDLINRATSGVGGGAMPPGGRPMPPGGPARPGMPPPVRR

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=554779 J8C00_RS09450 WP_058867080.1 2210042..2211211(+) (pilT) [Chloracidobacterium sp. E]
ATGTCGTCAAACTTTGTGCTGAGCGACCTGCTTCGGAAAATGATCGAACTGGGCGGCTCAGACCTCCACATCACAACCAA
TTCACCACCCCAGGTTCGGGTTCACGGACATCTTCGTCCACTCGACTACCCCGAAATGACGCCGGCGGATACCAAGCAAC
TGGCCTACTCCGTGCTGACCGACGCCCAGAAGCACCGTTTCGAGGAGACCCTGGAACTCGACTTCTCGTTCGGTATCAAA
GGCATGTCCCGCTTCCGGGCCAACCTCTTCAACCAGCGCGGCGCTGTGGCAGCGGTCTTCCGGGCCATTCCCTACGAAAT
CCGCACCTTTGAAGACTTGGGACTGCCACCGGTAGTCAGAAAGCTGTGTGAAAAGCCGCGTGGTCTCATTCTGGTCACCG
GGCCGACGGGTTCCGGCAAGTCCACCACCCTGGCTTCGATGATCAACAAAATCAACGAAGACCGGCACGAGCACATCATC
ACCATCGAAGACCCCATCGAGTTTCTCCATCCCCACAAGAACTGCCTCGTCAACCAGCGCGAAGTCCACGCCGACACACA
CAGTTTTGCCAACTCCCTGCGCGCTGCCCTGCGTCAGGACCCTGATGTGGTGCTCATCGGGGAGATGCGCGACCTGGAAA
CCGTCGAAACGGCGCTGCGGATCGCCGAAACCGGACACCTGACCTTCGGCACGCTGCACACGAACTCGGCTTACTCGACC
ATCAACCGTATCATTGACATCTTTCCGTCCCACCAGCAGTCCCAGATTCGGACGCAGCTCAGCCTCGTTCTCGAAGGCGT
GCTGTGCCAGTCGCTCCTCCCCAAAGCCAATGGGCAGGGACGCTGCATGGCGCTTGAAATCCTCGTCCCGAACTCGGCCA
TCCGCAACCTCATCCGGGAAGACAAAATCCACCAGATTTACGGGATGATGCAAACCGGGCAGGACAAGTTCGGGATGCAG
ACCTTCAATCAGTCGCTGCTCAGCTTGTACCTCAGCCGTCAGATTACGCTCGAAGTGGCCATGGCGCGGTCGCACAACCC
CGACGAGTTACAGGACCTCATCAACCGTGCCACCAGCGGCGTCGGCGGCGGGGCGATGCCCCCTGGCGGGCGGCCCATGC
CCCCGGGTGGCCCGGCGCGCCCCGGCATGCCCCCTCCGGTTCGCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

96.144

0.481
  pilT Legionella pneumophila strain Lp02

54.094

87.918

0.476
  pilT Legionella pneumophila strain ERS1305867

54.094

87.918

0.476
  pilT Neisseria meningitidis 8013

50.581

88.432

0.447
  pilT Pseudomonas stutzeri DSM 10701

50.581

88.432

0.447
  pilT Acinetobacter baylyi ADP1

50.581

88.432

0.447
  pilT Acinetobacter baumannii strain A118

50.291

88.432

0.445
  pilT Acinetobacter nosocomialis M2

50.291

88.432

0.445
  pilT Acinetobacter baumannii D1279779

50.291

88.432

0.445
  pilT Neisseria gonorrhoeae MS11

50

88.432

0.442
  pilT Pseudomonas aeruginosa PAK

49.128

88.432

0.434
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.411

82.005

0.422
  pilT Vibrio cholerae strain A1552

51.411

82.005

0.422
  pilU Pseudomonas stutzeri DSM 10701

44.058

88.689

0.391
  pilU Vibrio cholerae strain A1552

42.651

89.203

0.38
  pilU Acinetobacter baylyi ADP1

41.21

89.203

0.368