Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   J4Q40_RS05485 Genome accession   NZ_CP071891
Coordinates   1199173..1199598 (-) Length   141 a.a.
NCBI ID   WP_208151338.1    Uniprot ID   -
Organism   Xanthomonas oryzae pv. oryzae strain T7133     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1196809..1210385 1199173..1199598 within 0


Gene organization within MGE regions


Location: 1196809..1210385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4Q40_RS05475 - 1196809..1197438 (-) 630 WP_011408070.1 hypothetical protein -
  J4Q40_RS05480 - 1197449..1199038 (-) 1590 WP_011408069.1 phosphoethanolamine transferase -
  J4Q40_RS05485 pilA2 1199173..1199598 (-) 426 WP_208151338.1 pilin Machinery gene
  J4Q40_RS05490 pilC 1199953..1201212 (+) 1260 WP_011408067.1 type II secretion system F family protein Machinery gene
  J4Q40_RS05495 - 1201219..1202082 (+) 864 WP_011258358.1 A24 family peptidase -
  J4Q40_RS05500 coaE 1202096..1202704 (+) 609 WP_011258357.1 dephospho-CoA kinase -
  J4Q40_RS05505 - 1202775..1204094 (+) 1320 WP_115801881.1 IS701-like element ISXo15 family transposase -
  J4Q40_RS05510 - 1204655..1204957 (-) 303 WP_158645227.1 hypothetical protein -
  J4Q40_RS05515 - 1204941..1205897 (-) 957 WP_041182041.1 IS30-like element IS1112a family transposase -
  J4Q40_RS05520 - 1205987..1206208 (-) 222 Protein_1074 IS4 family transposase -
  J4Q40_RS05525 - 1206326..1206493 (-) 168 Protein_1075 IS701 family transposase -
  J4Q40_RS05530 - 1206589..1207557 (+) 969 WP_011258802.1 IS5-like element ISXo1 family transposase -
  J4Q40_RS05535 - 1207727..1208095 (-) 369 WP_011408064.1 DUF6869 domain-containing protein -
  J4Q40_RS05540 - 1208101..1209534 (-) 1434 Protein_1078 RHS repeat-associated core domain-containing protein -
  J4Q40_RS05545 - 1209611..1209940 (-) 330 WP_146772721.1 hypothetical protein -
  J4Q40_RS05550 - 1209937..1210236 (-) 300 WP_125168747.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14412.74 Da        Isoelectric Point: 9.0090

>NTDB_id=548839 J4Q40_RS05485 WP_208151338.1 1199173..1199598(-) (pilA2) [Xanthomonas oryzae pv. oryzae strain T7133]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKLSEALTMSAPAKLAVTETASSLGGVANVTLANSGYSFPGAAK
YVSNVTIADGTGLVTVTSTVPNAAGTILLTPKDVGGGQLKWTCSSAILTKYLPAECRSSGT

Nucleotide


Download         Length: 426 bp        

>NTDB_id=548839 J4Q40_RS05485 WP_208151338.1 1199173..1199598(-) (pilA2) [Xanthomonas oryzae pv. oryzae strain T7133]
ATGAAGAAGCAGCAAGGCTTTACACTTATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCTGCCATCGCGCT
GCCGGCTTATCAGGACTACACCACTCGTGCCAAGCTCAGTGAAGCGCTGACGATGTCGGCGCCTGCCAAACTTGCGGTAA
CGGAGACTGCATCGTCGCTCGGCGGTGTTGCTAATGTTACTCTCGCGAATTCGGGCTACTCATTCCCGGGTGCCGCGAAG
TATGTCAGCAACGTCACCATCGCCGATGGTACGGGCCTAGTCACTGTAACTTCTACCGTGCCGAACGCAGCCGGTACCAT
CCTGCTGACCCCGAAGGATGTCGGTGGCGGCCAGCTGAAGTGGACCTGCTCTTCCGCCATCCTCACCAAGTACCTGCCGG
CGGAGTGCCGTAGCTCGGGCACCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

50.735

96.454

0.489
  pilA2 Legionella pneumophila str. Paris

50.735

96.454

0.489
  pilA Ralstonia pseudosolanacearum GMI1000

41.212

100

0.482
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.054

100

0.447
  pilE Neisseria gonorrhoeae strain FA1090

37.278

100

0.447
  comP Acinetobacter baylyi ADP1

42.857

100

0.447
  pilE Neisseria gonorrhoeae MS11

37.736

100

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.041

100

0.404
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.222

95.745

0.404
  pilA/pilA1 Eikenella corrodens VA1

37.333

100

0.397
  pilA Pseudomonas aeruginosa PAK

36.667

100

0.39
  pilA Acinetobacter baumannii strain A118

37.589

100

0.376
  pilA Vibrio cholerae C6706

31.875

100

0.362
  pilA Vibrio cholerae strain A1552

31.875

100

0.362
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

31.875

100

0.362
  pilA Vibrio parahaemolyticus RIMD 2210633

40.157

90.071

0.362