Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J4O68_RS16250 Genome accession   NZ_CP071876
Coordinates   3373186..3374166 (+) Length   326 a.a.
NCBI ID   WP_045332866.1    Uniprot ID   A0A837FII0
Organism   Enterobacter hormaechei strain GX4-8L     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3368186..3379166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4O68_RS16210 (J4O68_16190) - 3368216..3368542 (+) 327 WP_003862421.1 YggL family protein -
  J4O68_RS16215 (J4O68_16195) - 3368600..3369316 (+) 717 WP_003862419.1 DUF2884 domain-containing protein -
  J4O68_RS16220 (J4O68_16200) - 3369429..3369791 (-) 363 WP_235155219.1 endonuclease domain-containing protein -
  J4O68_RS16225 (J4O68_16205) hemW 3369848..3370996 (-) 1149 WP_032610104.1 radical SAM family heme chaperone HemW -
  J4O68_RS16230 (J4O68_16210) - 3370989..3371582 (-) 594 WP_022651831.1 XTP/dITP diphosphatase -
  J4O68_RS16235 (J4O68_16215) yggU 3371586..3371882 (-) 297 WP_003860023.1 DUF167 family protein YggU -
  J4O68_RS16240 (J4O68_16220) - 3371879..3372445 (-) 567 WP_003860024.1 YggT family protein -
  J4O68_RS16245 (J4O68_16225) - 3372467..3373168 (-) 702 WP_003860026.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4O68_RS16250 (J4O68_16230) pilT 3373186..3374166 (+) 981 WP_045332866.1 type IV pilus twitching motility protein PilT Machinery gene
  J4O68_RS16255 (J4O68_16235) ruvX 3374177..3374593 (-) 417 WP_003860030.1 Holliday junction resolvase RuvX -
  J4O68_RS16260 (J4O68_16240) - 3374593..3375153 (-) 561 WP_100154209.1 YqgE/AlgH family protein -
  J4O68_RS16265 (J4O68_16245) gshB 3375228..3376175 (-) 948 WP_048209588.1 glutathione synthase -
  J4O68_RS16270 (J4O68_16250) rsmE 3376203..3376934 (-) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J4O68_RS16275 (J4O68_16255) endA 3376986..3377693 (-) 708 WP_003860034.1 deoxyribonuclease I -
  J4O68_RS16280 (J4O68_16260) - 3377788..3378285 (-) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35749.75 Da        Isoelectric Point: 6.2344

>NTDB_id=548718 J4O68_RS16250 WP_045332866.1 3373186..3374166(+) (pilT) [Enterobacter hormaechei strain GX4-8L]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLADRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGLHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=548718 J4O68_RS16250 WP_045332866.1 3373186..3374166(+) (pilT) [Enterobacter hormaechei strain GX4-8L]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCTCCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCCTGGTGGGCAAATGGGCAGGTTGATTTTGCCGTTACCCTCGCAGACCGTCAGCGCCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGTGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCTGCACAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGATACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAACTGGCGGGAAGCCTGCGTGCGGTTCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A837FII0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.653

99.08

0.433
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38
  pilB Haemophilus influenzae 86-028NP

33.146

100

0.362