Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J4N37_RS13615 Genome accession   NZ_CP071855
Coordinates   2862869..2863909 (-) Length   346 a.a.
NCBI ID   WP_010450347.1    Uniprot ID   A0A2K7SNY4
Organism   Vibrio sp. SCSIO 43153     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2857869..2868909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N37_RS13590 (J4N37_13460) rsmE 2858647..2859378 (+) 732 WP_038886322.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J4N37_RS13595 (J4N37_13465) gshB 2859393..2860343 (+) 951 WP_045389733.1 glutathione synthase -
  J4N37_RS13600 (J4N37_13470) - 2860472..2861035 (+) 564 WP_010450344.1 YqgE/AlgH family protein -
  J4N37_RS13605 (J4N37_13475) ruvX 2861083..2861508 (+) 426 WP_010450345.1 Holliday junction resolvase RuvX -
  J4N37_RS13610 (J4N37_13480) pilU 2861733..2862839 (-) 1107 WP_010450346.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J4N37_RS13615 (J4N37_13485) pilT 2862869..2863909 (-) 1041 WP_010450347.1 type IV pilus twitching motility protein PilT Machinery gene
  J4N37_RS13620 (J4N37_13490) - 2863938..2864648 (+) 711 WP_010450348.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4N37_RS13625 (J4N37_13495) proC 2864779..2865597 (+) 819 WP_071235275.1 pyrroline-5-carboxylate reductase -
  J4N37_RS13630 (J4N37_13500) - 2865651..2866208 (+) 558 WP_182027296.1 YggT family protein -
  J4N37_RS13635 (J4N37_13505) yggU 2866208..2866498 (+) 291 WP_005425805.1 DUF167 family protein YggU -
  J4N37_RS13640 (J4N37_13510) - 2866558..2866989 (+) 432 WP_005425804.1 DUF4426 domain-containing protein -
  J4N37_RS13645 (J4N37_13515) - 2867095..2867697 (+) 603 WP_005436525.1 XTP/dITP diphosphatase -
  J4N37_RS13650 (J4N37_13520) hemW 2867716..2868888 (+) 1173 WP_045480178.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38662.17 Da        Isoelectric Point: 6.5043

>NTDB_id=548425 J4N37_RS13615 WP_010450347.1 2862869..2863909(-) (pilT) [Vibrio sp. SCSIO 43153]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFRKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVTREEVDKKIEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=548425 J4N37_RS13615 WP_010450347.1 2862869..2863909(-) (pilT) [Vibrio sp. SCSIO 43153]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTGAGAAAACTGGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGACTTCTCATTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCCTTCAACCAATCTCGCGGTTGTGCGGCTGTGTTCCGTACCATCCCAAGTAGTATCCCAACACTGGA
AGAGCTAGAAGCTCCAGAGATCTTCAGAAAGATCGCCAATGCCGAAAAAGGTTTGGTACTGGTTACCGGTCCTACGGGTT
CTGGTAAATCGACTACCTTGGCTGCCATTGTTGACTACATTAACCGTAATCACAATAAGCATGTCCTAACAATTGAAGAT
CCGATTGAATTTGTTCACACCAACAATAAGTGTCTGATCAACCAACGTGAAGTACACCGTGATACACACAGCTTCCAAAA
CGCACTGCGCAGCGCATTGCGTGAAGACCCGGATGTAATTCTAGTTGGTGAGATGCGTGATAAAGAGACCATCAGTTTGG
CGCTGACAGCCGCTGAAACGGGCCACCTTGTTTTCGGTACGCTGCACACTAGCTCAGCAGCGAAAACCATCGACCGTATT
ATTGACGTCTTCCCTGGTAGTGACAAAGACATGGTGCGTTCGATGCTGTCTGAATCATTGCGTTCGGTTATCGCGCAAAA
ACTATTAAAACGTAACGGCGGCGGTCGTATTGCTTGTCATGAAATCATGATGGCGACACCTGCTATCCGTAACCTTATTC
GAGAAGATAAAGTCGCACAGATGTACTCAATCATTCAAACTGGTGCGGCGCACGGCATGCAAACCATGGAGCAAAACGCT
CGCCAATTGATGGCGCAAGGTATGGTTACTCGTGAAGAAGTGGATAAGAAAATCGAAATTGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K7SNY4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

71.818

95.376

0.685
  pilT Acinetobacter baumannii strain A118

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.733

95.087

0.682
  pilT Acinetobacter nosocomialis M2

71.515

95.376

0.682
  pilT Pseudomonas aeruginosa PAK

68.529

98.266

0.673
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.399

0.673
  pilT Legionella pneumophila strain Lp02

67.372

95.665

0.645
  pilT Legionella pneumophila strain ERS1305867

67.372

95.665

0.645
  pilT Neisseria meningitidis 8013

65.465

96.243

0.63
  pilT Neisseria gonorrhoeae MS11

63.824

98.266

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.143

100

0.506
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393