Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J4N37_RS13610 Genome accession   NZ_CP071855
Coordinates   2861733..2862839 (-) Length   368 a.a.
NCBI ID   WP_010450346.1    Uniprot ID   A0A2K7SNZ2
Organism   Vibrio sp. SCSIO 43153     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2856733..2867839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N37_RS13580 (J4N37_13450) - 2857158..2857655 (+) 498 WP_045389730.1 SprT family zinc-dependent metalloprotease -
  J4N37_RS13585 (J4N37_13455) endA 2857805..2858500 (+) 696 WP_010450340.1 deoxyribonuclease I -
  J4N37_RS13590 (J4N37_13460) rsmE 2858647..2859378 (+) 732 WP_038886322.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J4N37_RS13595 (J4N37_13465) gshB 2859393..2860343 (+) 951 WP_045389733.1 glutathione synthase -
  J4N37_RS13600 (J4N37_13470) - 2860472..2861035 (+) 564 WP_010450344.1 YqgE/AlgH family protein -
  J4N37_RS13605 (J4N37_13475) ruvX 2861083..2861508 (+) 426 WP_010450345.1 Holliday junction resolvase RuvX -
  J4N37_RS13610 (J4N37_13480) pilU 2861733..2862839 (-) 1107 WP_010450346.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J4N37_RS13615 (J4N37_13485) pilT 2862869..2863909 (-) 1041 WP_010450347.1 type IV pilus twitching motility protein PilT Machinery gene
  J4N37_RS13620 (J4N37_13490) - 2863938..2864648 (+) 711 WP_010450348.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4N37_RS13625 (J4N37_13495) proC 2864779..2865597 (+) 819 WP_071235275.1 pyrroline-5-carboxylate reductase -
  J4N37_RS13630 (J4N37_13500) - 2865651..2866208 (+) 558 WP_182027296.1 YggT family protein -
  J4N37_RS13635 (J4N37_13505) yggU 2866208..2866498 (+) 291 WP_005425805.1 DUF167 family protein YggU -
  J4N37_RS13640 (J4N37_13510) - 2866558..2866989 (+) 432 WP_005425804.1 DUF4426 domain-containing protein -
  J4N37_RS13645 (J4N37_13515) - 2867095..2867697 (+) 603 WP_005436525.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41321.45 Da        Isoelectric Point: 6.7730

>NTDB_id=548424 J4N37_RS13610 WP_010450346.1 2861733..2862839(-) (pilU) [Vibrio sp. SCSIO 43153]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLTENDVALLLDSAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGTLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=548424 J4N37_RS13610 WP_010450346.1 2861733..2862839(-) (pilU) [Vibrio sp. SCSIO 43153]
ATGGACCTGAATAAATTTCTCGAAGGCATGCTGGCGTTAAAAGCGTCGGATCTCTATATCACTGTTGGTGCACCGATCTT
GTTTCGTGTCGATGGCGAACTGCGCCCGCAAGGCGATAAGTTGACTGAAAATGACGTCGCCTTGTTGCTTGACAGCGCGA
TGGACCCAGAGCGACGTCAGGAGTTTCGCAAAAGCCGTGAGTCTAACTTTGCCATTGTCCGTGATTGTGGTCGCTTCCGT
GTCAGTGCCTTTTTCCAACGTGAATTGCCGGGAGCAGTCATTCGTCGTATTGAAACCAATATTCCGACGTTTGAGCAACT
CAAGTTACCTTTGGTGCTGCAAGATCTTGCCATTGCCAAGCGTGGTTTAGTGCTGGTGGTTGGTGCAACTGGTTCTGGTA
AGTCAACGACTATGGCGGCGATGACGGGCTATCGAAACAACAACAAAACTGGCCATATCCTGACGGTAGAAGACCCGATT
GAATTTGTGCACGAGCATAAGCGTTGTATCGTGACACAGCGTGAAGTGGGCCTAGACACCGACAGCTATGAGGTGGCGTT
GAAAAACTCGTTACGTCAGGCTCCAGACATGATTTTGATTGGTGAGATCCGTAGCCGAGAAACGATGGAATACGCAATGA
CCTTTGCTGAGACAGGTCACTTGTGTATGGCGACACTGCACGCCAACAATGCAAACCAAGCGCTTGAGCGTATTTTGCAC
CTAGTGCCAAAAGATCAGAAAGATCAGTTCCTGTTTGATCTGTCGATGAACTTAAAAGGTGTTGTCGGTCAGCAGTTGAT
CCGCGATAAAAACGGTCAAGGACGTCATGGCGTGTTTGAAATCTTATTAAACAGCCCGCGTGTTTCTGATCTGATCCGTC
GAGGTGATCTGCATGAGTTGAAATCAACCATGGCTCGCTCTAACGAATTTGGGATGTTGACCTTCGACCAATCGCTCTAC
AAATTGGTGATGCAAGGTAAGATCAGCGAAGAAGATGCATTGCACAGTGCGGATTCAGCCAACGATCTGCGTTTGATGTT
GAAGACGCAACGTGGTGAAGCCTTCTCTACAGGCACGCTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K7SNZ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.522

100

0.815
  pilU Pseudomonas stutzeri DSM 10701

58.757

96.196

0.565
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.86

93.478

0.391
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Pseudomonas stutzeri DSM 10701

40.936

92.935

0.38
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375