Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J4N43_RS02400 Genome accession   NZ_CP071842
Coordinates   498728..499768 (+) Length   346 a.a.
NCBI ID   WP_005381015.1    Uniprot ID   A0A2I3C9Z7
Organism   Vibrio sp. SCSIO 43009     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 493728..504768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N43_RS02370 (J4N43_02365) - 494839..495441 (-) 603 WP_006743095.1 XTP/dITP diphosphatase -
  J4N43_RS02375 (J4N43_02370) - 495560..495991 (-) 432 WP_053350811.1 DUF4426 domain-containing protein -
  J4N43_RS02380 (J4N43_02375) yggU 496120..496410 (-) 291 WP_005381011.1 DUF167 family protein YggU -
  J4N43_RS02385 (J4N43_02380) - 496410..496967 (-) 558 WP_005381012.1 YggT family protein -
  J4N43_RS02390 (J4N43_02385) proC 497020..497838 (-) 819 WP_062730829.1 pyrroline-5-carboxylate reductase -
  J4N43_RS02395 (J4N43_02390) - 497998..498699 (-) 702 WP_062730830.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4N43_RS02400 (J4N43_02395) pilT 498728..499768 (+) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  J4N43_RS02405 (J4N43_02400) pilU 499799..500905 (+) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J4N43_RS02410 (J4N43_02405) ruvX 500967..501392 (-) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  J4N43_RS02415 (J4N43_02410) - 501428..501991 (-) 564 WP_005381020.1 YqgE/AlgH family protein -
  J4N43_RS02420 (J4N43_02415) gshB 502120..503070 (-) 951 WP_005381021.1 glutathione synthase -
  J4N43_RS02425 (J4N43_02420) rsmE 503085..503816 (-) 732 WP_053350809.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J4N43_RS02430 (J4N43_02425) endA 503930..504625 (-) 696 WP_017635582.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38532.02 Da        Isoelectric Point: 6.5039

>NTDB_id=548193 J4N43_RS02400 WP_005381015.1 498728..499768(+) (pilT) [Vibrio sp. SCSIO 43009]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=548193 J4N43_RS02400 WP_005381015.1 498728..499768(+) (pilT) [Vibrio sp. SCSIO 43009]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCGGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTAAGAAAACTAGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAGTTGCATAACGTTGGCCGT
TTCCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTATTCCGTACCATTCCAAGTAGCATTCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTGGTCACTGGCCCAACAGGTT
CAGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCACGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATTAACCAGCGTGAAGTTCACCGTGACACCCATAGTTTCCAAAA
CGCACTGCGCAGCGCATTGCGCGAAGACCCAGACGTTATTCTGGTTGGTGAGATGCGTGACAAAGAAACCATCAGCCTTG
CCTTAACGGCGGCAGAAACGGGTCACTTAGTTTTCGGGACGTTGCACACCAGCTCGGCGGCAAAAACCATTGACCGTATT
ATCGATGTGTTTCCTGGTAGTGATAAAGATATGGTTCGCTCGATGCTATCTGAGTCCCTTCGTTCGGTTATCGCGCAAAA
GCTTCTGAAGCGTAATGGTGGTGGCCGTATTGCATGCCATGAAATTATGATGGCAACGCCTGCGATTCGCAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAAACAGGTGCGGCTCACGGCATGCAAACCATGGAGCAAAATGCT
CGTCAGTTAATGGCGCAAGGCATGGTTGCTCGTGAAGAGGTCGATAGTAAGATCGGATTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3C9Z7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

99.711

0.867
  pilT Vibrio cholerae strain A1552

86.957

99.711

0.867
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.429

95.087

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.399

0.671
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

91.908

0.486
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361