Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J4N40_RS02710 Genome accession   NZ_CP071840
Coordinates   556073..557179 (+) Length   368 a.a.
NCBI ID   WP_005381016.1    Uniprot ID   A0A2I3C9Z5
Organism   Vibrio sp. SCSIO 43097     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 551073..562179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N40_RS02675 (J4N40_02675) - 551113..551715 (-) 603 WP_005381009.1 XTP/dITP diphosphatase -
  J4N40_RS02680 (J4N40_02680) - 551834..552265 (-) 432 WP_005381010.1 DUF4426 domain-containing protein -
  J4N40_RS02685 (J4N40_02685) yggU 552394..552684 (-) 291 WP_065274862.1 DUF167 family protein YggU -
  J4N40_RS02690 (J4N40_02690) - 552684..553241 (-) 558 WP_005381012.1 YggT family protein -
  J4N40_RS02695 (J4N40_02695) proC 553294..554112 (-) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  J4N40_RS02700 (J4N40_02700) - 554272..554973 (-) 702 WP_115386296.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4N40_RS02705 (J4N40_02705) pilT 555002..556042 (+) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  J4N40_RS02710 (J4N40_02710) pilU 556073..557179 (+) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J4N40_RS02715 (J4N40_02715) ruvX 557241..557666 (-) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  J4N40_RS02720 (J4N40_02720) - 557702..558265 (-) 564 WP_005381020.1 YqgE/AlgH family protein -
  J4N40_RS02725 (J4N40_02725) gshB 558393..559343 (-) 951 WP_005381021.1 glutathione synthase -
  J4N40_RS02730 (J4N40_02730) rsmE 559357..560088 (-) 732 WP_025768830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J4N40_RS02735 (J4N40_02735) endA 560202..560897 (-) 696 WP_005384533.1 deoxyribonuclease I -
  J4N40_RS02740 (J4N40_02740) - 561048..561545 (-) 498 WP_115386294.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41254.36 Da        Isoelectric Point: 6.5943

>NTDB_id=548152 J4N40_RS02710 WP_005381016.1 556073..557179(+) (pilU) [Vibrio sp. SCSIO 43097]
MDLDKFLEGMLALKASDLYITVGAPILFRVDGELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=548152 J4N40_RS02710 WP_005381016.1 556073..557179(+) (pilU) [Vibrio sp. SCSIO 43097]
ATGGATCTAGATAAATTTCTCGAAGGCATGTTGGCGCTGAAAGCGTCGGATCTTTACATCACGGTTGGAGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAAAAACTCACCGAGAGTGATGTGGCTGCATTACTCGACAGCGCTA
TGGAACCAGATCGGCGTCAGGAATTTCGTAAAAGTCGCGAGTCGAATTTTGCCATTGTGAGAGATTGTGGTCGCTTCCGT
GTGAGTGCCTTTTTTCAACGCGAGTTACCAGGCGCAGTGATTCGTCGTATTGAAACTAATATTCCTACTTTTGAGCAGTT
AAAGTTACCGTTAGTGTTGCAAGATTTGGCGATAGCTAAGCGTGGTTTGGTGCTGGTGGTCGGTGCTACTGGATCGGGTA
AATCGACCACCATGGCGGCGATGACAGGCTATCGAAACAGCAATAAAACCGGGCACATTTTGACGGTCGAAGATCCGATC
GAGTTCGTGCATGAACACAAGCGCTGTATCGTGACTCAACGTGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGCCAAGCACCAGATATGATTTTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACCTGTGTATGGCAACGTTGCACGCCAACAATGCAAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGATTTGTCGATGAACCTAAAAGGCGTGGTTGGTCAGCAGCTGAT
CCGAGATAAAAATGGGCAGGGACGTCATGGTGTATTTGAGATCTTGCTAAATAGTCCACGTGTGTCGGATTTGATTCGTC
GCGGAGATTTACATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTTTAT
AAGTTAGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTACATAGTGCCGACTCAGCGAATGATCTGCGCCTGATGTT
GAAAACGCAGCGTGGTGAAGCATTCTCTACGGGCAGTTTGGCAAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3C9Z5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.337

100

0.823
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.692

89.946

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361