Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JZM58_RS28215 Genome accession   NZ_CP071797
Coordinates   6318753..6319787 (-) Length   344 a.a.
NCBI ID   WP_007953348.1    Uniprot ID   J3FB13
Organism   Pseudomonas fluorescens strain YK-310     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6313753..6324787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JZM58_RS28185 (JZM58_28185) ruvX 6313977..6314414 (+) 438 WP_197867213.1 Holliday junction resolvase RuvX -
  JZM58_RS28190 (JZM58_28190) pyrR 6314496..6314999 (+) 504 WP_207984221.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  JZM58_RS28195 (JZM58_28195) - 6315027..6316031 (+) 1005 WP_007913665.1 aspartate carbamoyltransferase catalytic subunit -
  JZM58_RS28200 (JZM58_28200) - 6316028..6317299 (+) 1272 WP_207984222.1 dihydroorotase -
  JZM58_RS28205 (JZM58_28205) - 6317335..6317769 (-) 435 WP_007953343.1 TM2 domain-containing protein -
  JZM58_RS28210 (JZM58_28210) - 6318019..6318636 (+) 618 WP_007953345.1 C40 family peptidase -
  JZM58_RS28215 (JZM58_28215) pilT 6318753..6319787 (-) 1035 WP_007953348.1 type IV pilus twitching motility protein PilT Machinery gene
  JZM58_RS28220 (JZM58_28220) - 6319844..6320530 (+) 687 WP_207984223.1 YggS family pyridoxal phosphate-dependent enzyme -
  JZM58_RS28225 (JZM58_28225) proC 6320554..6321372 (+) 819 WP_207984224.1 pyrroline-5-carboxylate reductase -
  JZM58_RS28230 (JZM58_28230) - 6321383..6321973 (+) 591 WP_007953355.1 YggT family protein -
  JZM58_RS28235 (JZM58_28235) - 6321970..6322260 (+) 291 WP_207984225.1 DUF167 domain-containing protein -
  JZM58_RS28240 (JZM58_28240) - 6322326..6323465 (+) 1140 WP_207984226.1 homoserine O-acetyltransferase -
  JZM58_RS28245 (JZM58_28245) metW 6323473..6324093 (+) 621 WP_007953362.1 methionine biosynthesis protein MetW -
  JZM58_RS28250 (JZM58_28250) - 6324120..6324554 (+) 435 WP_207984227.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38071.74 Da        Isoelectric Point: 7.0157

>NTDB_id=547846 JZM58_RS28215 WP_007953348.1 6318753..6319787(-) (pilT) [Pseudomonas fluorescens strain YK-310]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=547846 JZM58_RS28215 WP_007953348.1 6318753..6319787(-) (pilT) [Pseudomonas fluorescens strain YK-310]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGTGTGGATGGCGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGACTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTCCCCGGCGTGGCGCGC
TTCCGGGTCAATGCCTTCAACCAGAACCGTGGCGCTGGCGCGGTGTTCCGGACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGCGACGTCTTTCGCAAGATCACCGATGCGCCGCGCGGGCTGGTGCTGGTGACCGGACCGACCGGCT
CCGGCAAGTCCACGACCCTGGCGGCGATGATCGATTACCTCAACACCCATCGTCATCACCACATCCTGACCATCGAAGAT
CCGATCGAATTCGTTCACGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGCAGTTTCGCCAC
CGCATTGCGATCGGCACTGCGTGAGGATCCGGACGTGATTCTGGTGGGTGAGATGCGCGATCTGGAAACCATCCGCCTGG
CACTCACCGCCGCCGAAACCGGGCACCTGGTGTTCGGCACCTTGCACACCACGTCGGCAGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCCGGGGACGAAAAGTCCATGGTGCGCTCGATGCTCTCGGAGTCGTTGCTGGCGGTGGTGTCGCAGAC
GCTGATCAAGAAGATCGGCGGCGGCCGGGTGGCGGCGCACGAGATCATGCTGGGGACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATTCAGACCGGCGGATCGCTGGGGATGCAGACACTCGACATGTGCCTG
AAGGATCTGGTGACCAAGGGTTTGATCAGCCGCGAACATGCGCGGGAGAAGGCGCGCACGCCGGATAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J3FB13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398