Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J4G45_RS04515 Genome accession   NZ_CP071770
Coordinates   766405..767442 (-) Length   345 a.a.
NCBI ID   WP_004975327.1    Uniprot ID   A0AAP9KIG7
Organism   Acinetobacter towneri strain GX5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 761405..772442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4G45_RS04485 (J4G45_04485) - 762178..762633 (+) 456 WP_154320417.1 bacteriohemerythrin -
  J4G45_RS04490 (J4G45_04490) - 762779..763864 (+) 1086 WP_179996017.1 hypothetical protein -
  J4G45_RS04495 (J4G45_04495) - 763861..764181 (+) 321 WP_004975319.1 RnfH family protein -
  J4G45_RS04500 (J4G45_04500) - 764238..764636 (-) 399 WP_004975323.1 outer membrane protein assembly factor BamE -
  J4G45_RS04505 (J4G45_04505) fur 764755..765192 (+) 438 WP_004975325.1 ferric iron uptake transcriptional regulator -
  J4G45_RS04510 (J4G45_04510) pilU 765265..766383 (-) 1119 WP_104852406.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J4G45_RS04515 (J4G45_04515) pilT 766405..767442 (-) 1038 WP_004975327.1 type IV pilus twitching motility protein PilT Machinery gene
  J4G45_RS04520 (J4G45_04520) - 767567..768256 (+) 690 WP_180044258.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4G45_RS04525 (J4G45_04525) - 768617..768892 (-) 276 WP_004975329.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38264.81 Da        Isoelectric Point: 6.5056

>NTDB_id=547627 J4G45_RS04515 WP_004975327.1 766405..767442(-) (pilT) [Acinetobacter towneri strain GX5]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEIRRINLPALEHKEVHKLVYDIMNDKQRRDYEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEDLGMGQIFKDICEYPRGLVLVTGPTGSGKSTTLAAMIDFINDNRYDHILTVED
PIEFVHQSKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQSLLKKNGGGRVAAHEIMIGIPAVRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KNLVSRGVISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=547627 J4G45_RS04515 WP_004975327.1 766405..767442(-) (pilT) [Acinetobacter towneri strain GX5]
ATGGATATTACAGAATTATTGGCGTTCTCCGCTAAAAACGGCGCTTCGGATTTACACTTATCTGCGGGCATGCCACCACT
GATTCGTGTGGATGGTGAAATTCGTCGAATTAACTTACCAGCATTAGAACATAAAGAAGTACACAAATTAGTGTACGACA
TTATGAATGATAAACAACGTCGTGATTATGAAGAAAATCTAGAAACGGACTTTTCTTTTGAAGTCCCAGGCGTGGCTCGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGTGCAGGTGCAGTATTCCGTACCATTCCTTCTAAAGTTTTGACCCTTGA
AGATTTAGGCATGGGACAAATCTTTAAAGATATTTGTGAATATCCACGTGGTTTGGTATTGGTGACAGGCCCAACAGGTT
CAGGTAAATCAACCACACTTGCCGCGATGATTGACTTTATTAATGACAATCGCTACGACCATATTCTGACCGTCGAAGAC
CCAATCGAATTTGTACACCAATCTAAAAAATGTTTGGTTAACCAACGAGAAGTACACCGTGATACGCATGGCTTTAACGA
AGCATTACGTTCGGCACTGCGTGAAGACCCTGACATTATTCTAGTTGGTGAGATGCGTGACTTAGAAACCATTCGTTTGG
CACTGACTGCTGCTGAAACGGGTCACTTGGTATTTGGTACGCTACACACCACCTCTGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTACGTGCCATGTTGTCTGAATCTTTACAAGCCGTCATTTCACAATC
CCTGCTCAAGAAAAATGGTGGTGGTCGTGTAGCTGCGCATGAAATCATGATTGGTATTCCTGCAGTGCGTAACCTGATTC
GTGAAAACAAAGTAGCTCAAATGTACTCGGCAATTCAGACTGGTGCCAACTACGGTATGACCACCCTTGACCAGAGTTTG
AAAAACTTGGTATCGCGCGGTGTGATTAGCCCACAAACGGCTCGTACGGTTGCAAAACAGCCTGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.203

100

0.942
  pilT Acinetobacter baumannii D1279779

93.913

100

0.939
  pilT Acinetobacter baumannii strain A118

93.913

100

0.939
  pilT Acinetobacter baylyi ADP1

86.047

99.71

0.858
  pilT Pseudomonas aeruginosa PAK

82.849

99.71

0.826
  pilT Pseudomonas stutzeri DSM 10701

82.558

99.71

0.823
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Neisseria meningitidis 8013

68.696

100

0.687
  pilT Neisseria gonorrhoeae MS11

68.406

100

0.684
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.75

97.391

0.67
  pilT Vibrio cholerae strain A1552

68.75

97.391

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.101

0.406
  pilU Vibrio cholerae strain A1552

41.493

97.101

0.403
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388