Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PPUTUW4_RS25965 Genome accession   NC_019670
Coordinates   5838907..5839941 (-) Length   344 a.a.
NCBI ID   WP_015097205.1    Uniprot ID   -
Organism   Pseudomonas sp. UW4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5833907..5844941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPUTUW4_RS25935 (PputUW4_05109) ruvX 5834060..5834497 (+) 438 WP_007975412.1 Holliday junction resolvase RuvX -
  PPUTUW4_RS25940 (PputUW4_05110) pyrR 5834577..5835080 (+) 504 WP_015097201.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PPUTUW4_RS25945 (PputUW4_05111) - 5835108..5836127 (+) 1020 WP_007975410.1 aspartate carbamoyltransferase catalytic subunit -
  PPUTUW4_RS25950 (PputUW4_05112) - 5836124..5837395 (+) 1272 WP_015097202.1 dihydroorotase -
  PPUTUW4_RS25955 (PputUW4_05113) - 5837487..5837906 (-) 420 WP_015097203.1 NINE protein -
  PPUTUW4_RS25960 (PputUW4_05114) - 5838158..5838778 (+) 621 WP_015097204.1 C40 family peptidase -
  PPUTUW4_RS25965 (PputUW4_05115) pilT 5838907..5839941 (-) 1035 WP_015097205.1 type IV pilus twitching motility protein PilT Machinery gene
  PPUTUW4_RS25970 (PputUW4_05116) - 5839998..5840684 (+) 687 WP_015097206.1 YggS family pyridoxal phosphate-dependent enzyme -
  PPUTUW4_RS25975 (PputUW4_05117) proC 5840720..5841538 (+) 819 WP_007975403.1 pyrroline-5-carboxylate reductase -
  PPUTUW4_RS25980 (PputUW4_05118) - 5841549..5842139 (+) 591 WP_015097207.1 YggT family protein -
  PPUTUW4_RS25985 (PputUW4_05119) - 5842144..5842437 (+) 294 WP_015097208.1 DUF167 domain-containing protein -
  PPUTUW4_RS25990 (PputUW4_05120) - 5842622..5843761 (+) 1140 WP_015097209.1 homoserine O-acetyltransferase -
  PPUTUW4_RS25995 (PputUW4_05121) metW 5843769..5844389 (+) 621 WP_007975398.1 methionine biosynthesis protein MetW -
  PPUTUW4_RS26000 (PputUW4_05122) - 5844416..5844850 (+) 435 WP_015097210.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37657.21 Da        Isoelectric Point: 6.9823

>NTDB_id=54630 PPUTUW4_RS25965 WP_015097205.1 5838907..5839941(-) (pilT) [Pseudomonas sp. UW4]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHQQVQALIDAIMNDTQRADFEKHLEIDFSFEVPGVAR
FRANAFNQSRGAGAVFRTIAAKVQSMDELGMGEVFRKMTDAPRGLVLVTGPTGSGKSTTLAAMIDYLNNHRHHHILTIED
PIEFVHEPRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKVGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSAIQSGGALGMQTLDMSL
KGLVSRGVISRDHALEKARSPDSF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=54630 PPUTUW4_RS25965 WP_015097205.1 5838907..5839941(-) (pilT) [Pseudomonas sp. UW4]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAGGGGGCATCGGACCTGCATTTGTCTGCTGGCCTGCCGCCGAT
GATTCGGGTGGATGGCGATGTGCGGCGCATCAACCTGCCGGCCATGGACCACCAACAGGTGCAGGCGCTGATCGATGCCA
TCATGAATGACACCCAGCGGGCGGACTTTGAGAAGCATCTGGAAATCGATTTTTCCTTTGAAGTGCCCGGTGTGGCGCGT
TTTCGGGCCAATGCGTTCAATCAGAGCCGTGGCGCGGGTGCGGTGTTTCGCACCATTGCGGCGAAAGTGCAGAGCATGGA
CGAACTGGGCATGGGGGAAGTGTTTCGCAAAATGACCGACGCTCCCCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGTT
CCGGAAAATCCACCACGCTGGCGGCGATGATCGACTACCTCAATAACCATCGCCATCACCACATCCTCACCATTGAAGAC
CCTATCGAGTTTGTGCACGAGCCGCGCAAGTGCCTGATCAACCAGCGTGAGGTCCACCGCGATACCCGCAGCTTCGCCAC
GGCCTTGCGCTCGGCGTTGCGGGAGGATCCGGACGTGATTCTGGTGGGGGAGATGCGCGATCTGGAAACCATACGCCTGG
CGTTGACCGCCGCCGAAACCGGCCATCTGGTATTCGGCACGCTGCACACCACGTCGGCGGCAAAGACCATTGACCGGGTA
GTGGACGTGTTTCCGGGGGACGAGAAGTCAATGGTGCGCTCGATGCTCTCCGAGTCGCTGCTGGCGGTGGTGTCCCAGAC
GCTGGTGAAGAAGGTCGGCGGCGGACGCATCGCGGCCCACGAAATCATGCTGGGGACGTCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATTCAGTCGGGAGGGGCGTTGGGAATGCAGACGCTGGATATGAGCTTG
AAGGGCCTGGTGAGCAGGGGGGTGATCAGTCGTGATCATGCGCTGGAGAAAGCGCGGTCGCCGGATAGCTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

84.012

100

0.84
  pilT Pseudomonas aeruginosa PAK

82.558

100

0.826
  pilT Acinetobacter nosocomialis M2

76.453

100

0.765
  pilT Acinetobacter baumannii D1279779

76.163

100

0.762
  pilT Acinetobacter baumannii strain A118

76.163

100

0.762
  pilT Acinetobacter baylyi ADP1

75.581

100

0.756
  pilT Legionella pneumophila strain Lp02

68.314

100

0.683
  pilT Legionella pneumophila strain ERS1305867

68.314

100

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.301

95.64

0.663
  pilT Vibrio cholerae strain A1552

69.301

95.64

0.663
  pilT Neisseria meningitidis 8013

64.348

100

0.645
  pilT Neisseria gonorrhoeae MS11

64.058

100

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Vibrio cholerae strain A1552

40.87

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401

Multiple sequence alignment