Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J2N86_RS11375 Genome accession   NZ_CP071527
Coordinates   2578073..2579113 (-) Length   346 a.a.
NCBI ID   WP_252579584.1    Uniprot ID   -
Organism   Legionella lytica strain PCM 2298     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2573073..2584113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J2N86_RS11355 (J2N86_11365) - 2573535..2573975 (+) 441 WP_252579580.1 SMI1/KNR4 family protein -
  J2N86_RS11360 (J2N86_11370) - 2574100..2575278 (+) 1179 WP_252579581.1 IS4 family transposase -
  J2N86_RS11365 (J2N86_11375) - 2575425..2577185 (+) 1761 WP_252579582.1 ankyrin repeat domain-containing protein -
  J2N86_RS11370 (J2N86_11380) rph 2577255..2577962 (+) 708 WP_252579583.1 ribonuclease PH -
  J2N86_RS11375 (J2N86_11385) pilT 2578073..2579113 (-) 1041 WP_252579584.1 type IV pilus twitching motility protein PilT Machinery gene
  J2N86_RS11380 (J2N86_11390) - 2579237..2579923 (+) 687 WP_252579585.1 YggS family pyridoxal phosphate-dependent enzyme -
  J2N86_RS11385 (J2N86_11395) - 2580151..2580726 (+) 576 WP_252579586.1 YggT family protein -
  J2N86_RS11390 (J2N86_11400) - 2580772..2581329 (+) 558 WP_252579587.1 DUF2845 domain-containing protein -
  J2N86_RS11395 (J2N86_11405) - 2581351..2581632 (+) 282 WP_252579588.1 hypothetical protein -
  J2N86_RS11400 (J2N86_11410) - 2581818..2582873 (-) 1056 WP_252579589.1 enoyl-CoA hydratase/isomerase family protein -
  J2N86_RS11405 (J2N86_11415) - 2582876..2583652 (-) 777 WP_252579590.1 enoyl-CoA hydratase/isomerase family protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38458.06 Da        Isoelectric Point: 6.2962

>NTDB_id=546014 J2N86_RS11375 WP_252579584.1 2578073..2579113(-) (pilT) [Legionella lytica strain PCM 2298]
MDIIELLTSAVNNQASDLHLSSGVEPWIRVDGELYKMNLPVLEHSDLFAMIDELLSAQQKEELARHWELDFSFELAEFGR
FRVNVFHHLRGIAAAFRFIPTTIPSFSDLSLPPVFTELINYSNGLVLVTGPTGSGKSTTLAAMIDYINNSRHGHILTIED
PIEFVHLSNQCLVSQREVHHNTHSFHAALRSALREDPDVLLVGELRDLETIRLAMSAAETGHLVLGSLHTNSAVHAVNRI
LDVFPADEKAAARSILAESLRAVIAQRLVKRKEGRGRIAALEIMICTRAIRNLIREDKVAQMVSFLQTGKGGGMQTIDQH
LLELVRNGIISRDTVRDIAVDTGLFN

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=546014 J2N86_RS11375 WP_252579584.1 2578073..2579113(-) (pilT) [Legionella lytica strain PCM 2298]
ATGGATATAATCGAATTATTGACTTCAGCAGTTAACAATCAAGCTTCAGATTTACATTTGTCCTCTGGTGTAGAGCCCTG
GATTCGTGTTGATGGCGAATTATACAAAATGAACTTACCGGTTTTAGAGCATTCCGATTTATTCGCTATGATTGATGAAC
TTCTTAGTGCACAACAAAAGGAAGAGTTGGCAAGACATTGGGAACTTGATTTTTCTTTTGAACTTGCGGAATTTGGTCGT
TTCCGGGTTAATGTATTTCATCATTTGCGGGGTATTGCTGCGGCTTTTCGGTTTATTCCTACAACGATTCCCAGTTTTAG
CGATTTGAGTTTGCCACCTGTTTTTACTGAACTAATCAATTACTCTAACGGATTGGTATTAGTAACAGGACCAACGGGTT
CAGGAAAAAGTACAACCTTAGCGGCGATGATTGATTACATTAACAACTCACGCCATGGACATATTCTTACTATCGAAGAC
CCGATAGAGTTTGTGCACCTTAGTAATCAATGTTTAGTGAGTCAACGGGAAGTGCATCACAATACCCATAGTTTCCATGC
CGCACTACGTTCTGCTTTGCGTGAGGATCCAGATGTCTTGTTGGTGGGAGAATTGCGCGATTTAGAAACGATTCGATTAG
CTATGAGTGCGGCAGAAACTGGGCATTTGGTATTGGGGAGCTTACATACAAATTCTGCAGTTCATGCAGTTAATCGTATT
CTTGATGTCTTTCCTGCAGACGAAAAAGCGGCAGCACGTTCTATTTTAGCGGAATCATTAAGGGCGGTTATTGCACAAAG
GTTGGTAAAGCGAAAGGAGGGAAGAGGGCGCATTGCTGCTTTAGAAATCATGATTTGTACGAGGGCAATACGTAATTTAA
TTCGAGAAGATAAAGTAGCGCAGATGGTTTCTTTTTTGCAAACGGGGAAGGGGGGAGGGATGCAGACCATCGATCAGCAT
TTGTTGGAATTAGTGAGGAACGGGATTATATCCAGAGATACTGTACGTGATATAGCGGTGGATACGGGATTGTTTAATTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

64.638

99.711

0.645
  pilT Legionella pneumophila strain Lp02

64.638

99.711

0.645
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

59.821

97.11

0.581
  pilT Vibrio cholerae strain A1552

59.821

97.11

0.581
  pilT Acinetobacter baylyi ADP1

58.997

97.977

0.578
  pilT Acinetobacter nosocomialis M2

58.997

97.977

0.578
  pilT Pseudomonas aeruginosa PAK

58.997

97.977

0.578
  pilT Acinetobacter baumannii D1279779

58.997

97.977

0.578
  pilT Acinetobacter baumannii strain A118

58.997

97.977

0.578
  pilT Pseudomonas stutzeri DSM 10701

58.702

97.977

0.575
  pilT Neisseria meningitidis 8013

55.655

97.11

0.54
  pilT Neisseria gonorrhoeae MS11

55.357

97.11

0.538
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.507

99.711

0.454
  pilU Vibrio cholerae strain A1552

40.483

95.665

0.387
  pilU Pseudomonas stutzeri DSM 10701

40.181

95.665

0.384
  pilU Acinetobacter baylyi ADP1

38.554

95.954

0.37