Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J0B03_RS00765 Genome accession   NZ_CP071444
Coordinates   156414..157478 (-) Length   354 a.a.
NCBI ID   WP_207299997.1    Uniprot ID   A0A974XMJ2
Organism   Alkalibacter rhizosphaerae strain ES005     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 151414..162478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0B03_RS00750 (J0B03_00750) - 152848..153912 (-) 1065 WP_207299994.1 PilX N-terminal domain-containing pilus assembly protein -
  J0B03_RS12375 (J0B03_00755) - 154740..155084 (-) 345 WP_207299995.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  J0B03_RS00760 (J0B03_00760) - 155177..156391 (-) 1215 WP_207299996.1 type II secretion system F family protein -
  J0B03_RS00765 (J0B03_00765) pilT 156414..157478 (-) 1065 WP_207299997.1 type IV pilus twitching motility protein PilT Machinery gene
  J0B03_RS00770 (J0B03_00770) - 157492..159180 (-) 1689 WP_207299998.1 GspE/PulE family protein -
  J0B03_RS00775 (J0B03_00775) - 159256..159678 (-) 423 WP_207299999.1 methylglyoxal synthase -
  J0B03_RS00780 (J0B03_00780) - 159680..160912 (-) 1233 WP_207300950.1 U32 family peptidase -
  J0B03_RS00785 (J0B03_00785) - 160920..161600 (-) 681 WP_207300000.1 O-methyltransferase -
  J0B03_RS00790 (J0B03_00790) - 161602..161934 (-) 333 WP_207300001.1 YlbF family regulator -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39346.27 Da        Isoelectric Point: 7.2938

>NTDB_id=545343 J0B03_RS00765 WP_207299997.1 156414..157478(-) (pilT) [Alkalibacter rhizosphaerae strain ES005]
MKNDIDQLLKATIESKASDLHITVGLPPMIRIDGELHPLWDIAPYTQDETKQLATAMVNDSQRKALEETGQVDFSYSWSG
YGRFRVNAFKQRTSYTVALRVIGSGIPTMAELNLPKTMEQLTRINKGLILVTGPTGSGKSTTLASMIDIINSERSAHILT
LEDPIEYLHRHKKSMVNQREIGQDTTSYQDALRAALREDPDVILVGEMRDLDSIAIALTAAETGHLVLSTLHTIGAAKTI
DRVIDVFPPHQQQQVRTQMASVIQSVVSQQLLAKKSGHGRIVALEIMIANPAIRNLIREGKNHQINSILQMSGKAGMVTM
DSYLYDLYSKGVISMDNALFYSVDQEAMRKRMMV

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=545343 J0B03_RS00765 WP_207299997.1 156414..157478(-) (pilT) [Alkalibacter rhizosphaerae strain ES005]
ATGAAAAACGATATCGACCAACTGCTCAAAGCGACTATTGAAAGCAAGGCTTCCGACCTGCACATCACTGTAGGGCTGCC
ACCCATGATCCGAATCGACGGAGAGCTCCATCCTTTATGGGACATTGCTCCCTATACCCAGGACGAGACGAAACAGCTGG
CAACGGCCATGGTAAACGACAGCCAGAGAAAAGCCCTGGAAGAAACGGGACAAGTGGACTTCTCCTATTCCTGGTCCGGG
TACGGACGGTTCCGGGTCAACGCCTTCAAGCAGCGGACCAGCTATACCGTAGCTCTACGGGTCATCGGTTCCGGGATCCC
AACCATGGCAGAGTTGAACCTGCCAAAGACCATGGAACAACTGACGAGGATCAACAAGGGATTGATTTTGGTTACGGGTC
CTACCGGTAGTGGTAAATCCACCACCCTGGCCTCCATGATCGACATCATCAATAGCGAGCGAAGCGCCCATATCCTGACC
TTGGAGGATCCCATCGAATACCTTCACCGTCACAAAAAAAGCATGGTCAACCAACGGGAGATCGGCCAGGACACCACCAG
CTATCAGGATGCTCTACGAGCCGCCCTTCGGGAAGACCCGGATGTCATTCTGGTAGGGGAGATGCGGGACCTGGATTCCA
TCGCCATTGCACTGACGGCGGCAGAAACAGGCCACTTGGTCCTATCCACCCTGCACACCATTGGTGCCGCCAAGACCATT
GACCGGGTCATCGACGTCTTTCCGCCTCATCAGCAGCAACAGGTACGGACCCAGATGGCAAGCGTCATCCAGTCCGTTGT
ATCCCAACAGTTGTTGGCCAAGAAAAGCGGACATGGACGGATCGTGGCCTTGGAGATCATGATCGCCAACCCAGCTATCC
GAAACCTGATCCGGGAAGGAAAGAACCACCAGATCAATTCCATACTCCAAATGAGTGGCAAAGCAGGCATGGTCACCATG
GACAGCTATTTATATGATCTGTATTCCAAAGGAGTCATCAGCATGGACAATGCCCTGTTCTACTCCGTAGACCAGGAAGC
CATGCGAAAACGCATGATGGTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.537

94.633

0.497
  pilT Pseudomonas stutzeri DSM 10701

52.537

94.633

0.497
  pilT Acinetobacter baylyi ADP1

51.94

94.633

0.492
  pilT Acinetobacter nosocomialis M2

51.642

94.633

0.489
  pilT Acinetobacter baumannii D1279779

51.343

94.633

0.486
  pilT Acinetobacter baumannii strain A118

51.343

94.633

0.486
  pilT Legionella pneumophila strain Lp02

51.045

94.633

0.483
  pilT Legionella pneumophila strain ERS1305867

51.045

94.633

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.56

96.328

0.477
  pilT Neisseria meningitidis 8013

48.837

97.175

0.475
  pilT Neisseria gonorrhoeae MS11

48.547

97.175

0.472
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.303

93.22

0.469
  pilT Vibrio cholerae strain A1552

50.303

93.22

0.469
  pilU Vibrio cholerae strain A1552

41.349

96.328

0.398
  pilU Pseudomonas stutzeri DSM 10701

39.017

97.74

0.381
  pilU Acinetobacter baylyi ADP1

38.824

96.045

0.373