Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J0F83_RS20620 Genome accession   NZ_CP071218
Coordinates   4322764..4323786 (-) Length   340 a.a.
NCBI ID   WP_033639393.1    Uniprot ID   A0A379YJK8
Organism   Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S65_jyu2015     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4317764..4328786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0F83_RS20590 (J0F83_20590) - 4318565..4319077 (+) 513 WP_033639394.1 SprT family zinc-dependent metalloprotease -
  J0F83_RS20595 (J0F83_20595) endA 4319179..4319874 (+) 696 WP_015379043.1 deoxyribonuclease I -
  J0F83_RS20600 (J0F83_20600) rsmE 4319944..4320675 (+) 732 WP_004937458.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J0F83_RS20605 (J0F83_20605) gshB 4320686..4321636 (+) 951 WP_016930084.1 glutathione synthase -
  J0F83_RS20610 (J0F83_20610) - 4321782..4322345 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  J0F83_RS20615 (J0F83_20615) ruvX 4322345..4322767 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  J0F83_RS20620 (J0F83_20620) pilT 4322764..4323786 (-) 1023 WP_033639393.1 type IV pilus twitching motility protein PilT Machinery gene
  J0F83_RS20625 (J0F83_20625) - 4323807..4324514 (+) 708 WP_033639392.1 YggS family pyridoxal phosphate-dependent enzyme -
  J0F83_RS20630 (J0F83_20630) proC 4324534..4325355 (+) 822 WP_033639391.1 pyrroline-5-carboxylate reductase -
  J0F83_RS20635 (J0F83_20635) - 4325387..4325941 (+) 555 WP_004937436.1 YggT family protein -
  J0F83_RS20640 (J0F83_20640) yggU 4325938..4326228 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  J0F83_RS20645 (J0F83_20645) - 4326247..4326840 (+) 594 WP_004937429.1 XTP/dITP diphosphatase -
  J0F83_RS20650 (J0F83_20650) hemW 4326833..4327975 (+) 1143 WP_033639390.1 radical SAM family heme chaperone HemW -
  J0F83_RS20655 (J0F83_20655) - 4328012..4328446 (-) 435 WP_016930094.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36501.78 Da        Isoelectric Point: 7.2889

>NTDB_id=543932 J0F83_RS20620 WP_033639393.1 4322764..4323786(-) (pilT) [Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S65_jyu2015]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQNAGISLALRRIAGQSPSLAELAAPAIVPALLRRDDGLILVTGATGSGKSTTLAAMIDEINRHQQRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGERAGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=543932 J0F83_RS20620 WP_033639393.1 4322764..4323786(-) (pilT) [Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S65_jyu2015]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAACGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATTGTCCCGGCGTTGCTGCGGCGCGACGACGGGCTTATCTTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGTTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAGCCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGAGAGTGCGCGGGGGAAAGGGCGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A379YJK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Neisseria meningitidis 8013

50

100

0.5
  pilT Pseudomonas aeruginosa PAK

49.267

100

0.494
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Neisseria gonorrhoeae MS11

49.408

99.412

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.673

99.706

0.485
  pilT Legionella pneumophila strain Lp02

47.941

100

0.479
  pilT Legionella pneumophila strain ERS1305867

47.941

100

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.737

100

0.45
  pilU Pseudomonas stutzeri DSM 10701

38.348

99.706

0.382
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379