Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JY391_RS18595 Genome accession   NZ_CP071070
Coordinates   3912529..3913509 (-) Length   326 a.a.
NCBI ID   WP_199956972.1    Uniprot ID   -
Organism   Citrobacter sedlakii strain 3347689II     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3907529..3918509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JY391_RS18565 (JY391_18565) - 3908278..3908778 (+) 501 WP_211934806.1 SprT family zinc-dependent metalloprotease -
  JY391_RS18570 (JY391_18570) endA 3908870..3909577 (+) 708 WP_042290392.1 deoxyribonuclease I -
  JY391_RS18575 (JY391_18575) rsmE 3909649..3910380 (+) 732 WP_060570792.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JY391_RS18580 (JY391_18580) gshB 3910393..3911340 (+) 948 WP_042290397.1 glutathione synthase -
  JY391_RS18585 (JY391_18585) - 3911516..3912079 (+) 564 WP_042290400.1 YqgE/AlgH family protein -
  JY391_RS18590 (JY391_18590) ruvX 3912079..3912495 (+) 417 WP_042290401.1 Holliday junction resolvase RuvX -
  JY391_RS18595 (JY391_18595) pilT 3912529..3913509 (-) 981 WP_199956972.1 type IV pilus twitching motility protein PilT Machinery gene
  JY391_RS18600 (JY391_18600) - 3913527..3914231 (+) 705 WP_042290467.1 YggS family pyridoxal phosphate-dependent enzyme -
  JY391_RS18605 (JY391_18605) - 3914250..3914816 (+) 567 WP_042290404.1 YggT family protein -
  JY391_RS18610 (JY391_18610) yggU 3914813..3915103 (+) 291 WP_042290406.1 DUF167 family protein YggU -
  JY391_RS18615 (JY391_18615) - 3915111..3915704 (+) 594 WP_211934807.1 XTP/dITP diphosphatase -
  JY391_RS18620 (JY391_18620) hemW 3915697..3916833 (+) 1137 WP_211934808.1 radical SAM family heme chaperone HemW -
  JY391_RS18625 (JY391_18625) - 3917140..3918126 (+) 987 WP_211934809.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35863.03 Da        Isoelectric Point: 7.2104

>NTDB_id=542675 JY391_RS18595 WP_199956972.1 3912529..3913509(-) (pilT) [Citrobacter sedlakii strain 3347689II]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRLEAAPFPIPDMDSLLAQWLDARQQAVFQQEGQLDFAVTLTGNQRLRA
SAFRQQRGISLALRLLPSRCATLEALATPAALPELLASENGLILVTGATGSGKSTTLAAMVDWLNRHTDGHILTLEDPIE
YIYASQRCLIQQREIGMHCSSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKEPVRSQLAGSLRAVLSQKLEPDKQEGRVALFELLINTPATGNLIREGKTHQLPHVIQTGQQAGMMTFAQSWQQR
QAQGRV

Nucleotide


Download         Length: 981 bp        

>NTDB_id=542675 JY391_RS18595 WP_199956972.1 3912529..3913509(-) (pilT) [Citrobacter sedlakii strain 3347689II]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTAAGCAATGCGTGGCCGGCGCG
ATGGCGCAAGCGAGGACGGCTTGAGGCGGCGCCATTCCCTATACCGGATATGGACAGCCTGTTGGCGCAATGGCTTGATG
CGCGACAGCAGGCGGTGTTTCAGCAGGAAGGGCAGCTTGATTTCGCCGTCACGCTTACGGGTAACCAGCGCTTGCGGGCC
AGCGCCTTTCGTCAACAGCGGGGGATATCGCTGGCGCTGCGCTTGTTGCCCTCCCGTTGCGCCACCCTGGAAGCGCTGGC
GACGCCTGCGGCACTGCCTGAACTGCTTGCCAGCGAGAACGGTCTGATACTGGTGACCGGAGCGACGGGCAGCGGTAAAT
CCACCACCCTGGCGGCAATGGTGGACTGGCTCAACCGCCATACCGACGGACATATTCTGACGCTGGAAGATCCCATTGAG
TACATTTACGCCAGCCAACGCTGCCTGATTCAACAGCGTGAGATCGGTATGCATTGCTCATCGTTTGCCGCGGGATTACG
CGCGGCGCTGCGGGAAGACCCAGACGTTATTCTGCTCGGGGAACTGCGGGACAGCGAGACCATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGGCATCTGGTATTGGCGACGCTACATACCCGCGGCGCGGCGCAGGCCGTCGAGCGGCTGGTGGATACC
TTTCCGGCGCAGGAAAAAGAGCCGGTGCGCAGCCAACTGGCGGGCAGTCTGCGCGCCGTTCTGTCGCAAAAGCTGGAGCC
CGACAAGCAGGAAGGGCGCGTTGCGCTGTTTGAATTGCTGATCAACACCCCAGCGACGGGAAATTTAATTCGTGAGGGCA
AAACGCACCAACTACCCCATGTCATTCAGACCGGACAGCAGGCTGGAATGATGACCTTTGCCCAGAGTTGGCAGCAGCGT
CAGGCGCAGGGGCGAGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.632

100

0.491
  pilT Vibrio cholerae strain A1552

48.632

100

0.491
  pilT Neisseria meningitidis 8013

47.576

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.273

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

46.505

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.201

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

45.593

100

0.46
  pilT Acinetobacter baumannii strain A118

45.593

100

0.46
  pilT Acinetobacter nosocomialis M2

45.289

100

0.457
  pilT Legionella pneumophila strain ERS1305867

46.226

97.546

0.451
  pilT Legionella pneumophila strain Lp02

46.226

97.546

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.587

97.239

0.414
  pilU Vibrio cholerae strain A1552

40.062

98.773

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Acinetobacter baylyi ADP1

37.538

99.693

0.374