Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JYB86_RS11830 Genome accession   NZ_CP070997
Coordinates   2523260..2524297 (+) Length   345 a.a.
NCBI ID   WP_005179768.1    Uniprot ID   N9MH18
Organism   Acinetobacter indicus strain LYS68A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2474775..2522404 2523260..2524297 flank 856


Gene organization within MGE regions


Location: 2474775..2524297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JYB86_RS11620 (JYB86_11620) - 2474778..2474972 (+) 195 WP_168370238.1 hypothetical protein -
  JYB86_RS11625 (JYB86_11625) pgaD 2475071..2475508 (-) 438 WP_205779043.1 poly-beta-1,6-N-acetyl-D-glucosamine biosynthesis protein PgaD -
  JYB86_RS11630 (JYB86_11630) pgaC 2475541..2476800 (-) 1260 WP_205779044.1 poly-beta-1,6-N-acetyl-D-glucosamine synthase -
  JYB86_RS11635 (JYB86_11635) pgaB 2476819..2478744 (-) 1926 WP_228288739.1 poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB -
  JYB86_RS11640 (JYB86_11640) tehB 2479321..2480187 (+) 867 WP_205779046.1 SAM-dependent methyltransferase TehB -
  JYB86_RS11645 (JYB86_11645) - 2480325..2480435 (-) 111 WP_005179737.1 hypothetical protein -
  JYB86_RS11650 (JYB86_11650) - 2480623..2481862 (+) 1240 Protein_2262 IS256 family transposase -
  JYB86_RS11655 (JYB86_11655) - 2481983..2482405 (+) 423 Protein_2263 site-specific integrase -
  JYB86_RS11660 (JYB86_11660) - 2482771..2483157 (-) 387 Protein_2264 Fic family protein -
  JYB86_RS11665 (JYB86_11665) - 2483333..2484064 (+) 732 WP_205779047.1 Fic family protein -
  JYB86_RS11670 (JYB86_11670) - 2484683..2486386 (-) 1704 WP_205779048.1 LeoA/HP0731 family dynamin-like GTPase -
  JYB86_RS11675 (JYB86_11675) - 2486387..2488762 (-) 2376 WP_205779049.1 GTPase -
  JYB86_RS11680 (JYB86_11680) - 2488818..2489720 (-) 903 WP_205779050.1 helix-turn-helix transcriptional regulator -
  JYB86_RS11685 (JYB86_11685) - 2490604..2493480 (-) 2877 WP_205779051.1 type I restriction endonuclease subunit R -
  JYB86_RS11690 (JYB86_11690) - 2493509..2494420 (-) 912 WP_205779052.1 DUF5655 domain-containing protein -
  JYB86_RS11695 (JYB86_11695) - 2494438..2495289 (-) 852 WP_034585572.1 HNH endonuclease -
  JYB86_RS11700 (JYB86_11700) - 2495300..2496541 (-) 1242 WP_205779053.1 restriction endonuclease subunit S -
  JYB86_RS11705 (JYB86_11705) - 2496541..2498109 (-) 1569 WP_205779054.1 type I restriction-modification system subunit M -
  JYB86_RS11710 (JYB86_11710) - 2498313..2499374 (-) 1062 WP_180168789.1 HNH endonuclease -
  JYB86_RS11715 (JYB86_11715) - 2499377..2499964 (-) 588 WP_205779055.1 class I SAM-dependent methyltransferase -
  JYB86_RS11720 (JYB86_11720) - 2500037..2500267 (-) 231 WP_004811081.1 helix-turn-helix transcriptional regulator -
  JYB86_RS11725 (JYB86_11725) - 2500365..2501264 (-) 900 WP_205779056.1 hydrolase or metal-binding protein -
  JYB86_RS11730 (JYB86_11730) - 2501422..2501817 (+) 396 WP_075166718.1 transposase family protein -
  JYB86_RS11735 (JYB86_11735) - 2501765..2502235 (+) 471 WP_104470204.1 transposase family protein -
  JYB86_RS11740 (JYB86_11740) - 2502461..2503525 (-) 1065 WP_205779057.1 YqaJ viral recombinase family protein -
  JYB86_RS11745 (JYB86_11745) - 2503675..2504709 (-) 1035 WP_205779058.1 DUF932 domain-containing protein -
  JYB86_RS11750 (JYB86_11750) - 2504730..2505002 (-) 273 WP_205779059.1 hypothetical protein -
  JYB86_RS11755 (JYB86_11755) - 2505069..2505986 (-) 918 WP_205779060.1 inovirus-type Gp2 protein -
  JYB86_RS11760 (JYB86_11760) - 2506286..2507332 (-) 1047 WP_205779061.1 hypothetical protein -
  JYB86_RS11765 (JYB86_11765) - 2507620..2508779 (+) 1160 WP_205778996.1 IS3 family transposase -
  JYB86_RS11770 (JYB86_11770) - 2508739..2509062 (-) 324 WP_205779062.1 hypothetical protein -
  JYB86_RS11775 (JYB86_11775) - 2509338..2510474 (-) 1137 WP_205779792.1 tyrosine-type recombinase/integrase -
  JYB86_RS11785 (JYB86_11785) glpK 2511149..2512633 (+) 1485 WP_205779063.1 glycerol kinase GlpK -
  JYB86_RS11790 (JYB86_11790) - 2512754..2513251 (-) 498 WP_034597877.1 thioesterase family protein -
  JYB86_RS11795 (JYB86_11795) hemB 2513360..2514373 (+) 1014 WP_005179750.1 porphobilinogen synthase -
  JYB86_RS11800 (JYB86_11800) thiO 2514390..2515514 (+) 1125 WP_205779064.1 glycine oxidase ThiO -
  JYB86_RS11805 (JYB86_11805) - 2515569..2516030 (-) 462 WP_205779065.1 ABZJ_00895 family protein -
  JYB86_RS11810 (JYB86_11810) - 2516438..2517688 (+) 1251 WP_205779066.1 exonuclease SbcCD subunit D C-terminal domain-containing protein -
  JYB86_RS11815 (JYB86_11815) - 2517698..2521303 (+) 3606 WP_205779067.1 AAA family ATPase -
  JYB86_RS11820 (JYB86_11820) - 2521307..2522404 (+) 1098 WP_104488133.1 acyltransferase family protein -
  JYB86_RS11825 (JYB86_11825) - 2522450..2523136 (-) 687 WP_163143431.1 YggS family pyridoxal phosphate-dependent enzyme -
  JYB86_RS11830 (JYB86_11830) pilT 2523260..2524297 (+) 1038 WP_005179768.1 type IV pilus twitching motility protein PilT Machinery gene

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38237.74 Da        Isoelectric Point: 6.5051

>NTDB_id=542094 JYB86_RS11830 WP_005179768.1 2523260..2524297(+) (pilT) [Acinetobacter indicus strain LYS68A]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEDNLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFRDICDYPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYDHILTVED
PIEFVHQSKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMSTLDQSL
KALVSRGVISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=542094 JYB86_RS11830 WP_005179768.1 2523260..2524297(+) (pilT) [Acinetobacter indicus strain LYS68A]
ATGGATATTACAGAACTATTGGCCTTTTCGGCAAAAAATGGGGCATCGGATTTACACCTGTCGGCAGGCATGCCGCCACT
GATTCGTGTGGATGGTGAAGTACGCCGCATCAATTTGCCCGCCTTGGAACATAAAGAAGTCCACAAGCTGGTCTATGACA
TCATGAACGACAAACAGCGCCGTGATTATGAAGATAACCTGGAAACCGACTTCTCGTTTGAAGTCCCGGGCGTGGCGCGT
TTCCGTGTTAATGCATTTAACCAGAACCGCGGTGCAGGCGCGGTGTTTCGTACCATTCCCTCTAAAGTGCTGACCATTGA
AGATTTGGGAATGGGTCAAATCTTTAGAGATATCTGTGACTATCCACGTGGTCTAGTGCTAGTCACGGGGCCGACGGGTT
CGGGTAAATCCACCACCCTGGCGGCGATGATTGACTATATCAATGACAATCGCTATGACCACATCCTGACCGTGGAAGAC
CCGATTGAATTTGTGCATCAGTCTAAAAAATGTCTGGTCAACCAGCGTGAAGTGCATCGTGATACCCACGGCTTTAATGA
AGCACTACGTTCGGCGCTGCGTGAAGACCCGGATATTATTCTGGTCGGTGAGATGCGTGACCTGGAAACCATCCGTTTGG
CACTTACCGCGGCAGAAACCGGTCACCTGGTCTTTGGTACCCTGCATACCACCTCGGCGGCAAAAACCATTGACCGTGTG
ATTGACGTCTTTCCGGCCGAAGAAAAAGATATGGTGCGTGCCATGCTGTCTGAATCTTTACAGGCGGTCATTTCACAAAC
CCTGCTCAAGAAAAATGGCGGTGGCCGTGTGGCGGCGCATGAAATCATGATCGGGATTCCGGCCATCCGTAACCTGATTC
GTGAAAACAAGGTTGCACAGATGTACTCAGCCATTCAAACCGGTGCCAACTACGGCATGAGTACGCTGGACCAGAGTCTG
AAAGCCCTGGTCAGCCGTGGCGTGATCAGTCCGCAGACCGCGCGTACTGTGGCGAAACAGCCAGAATCGTTCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9MH18

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.783

100

0.948
  pilT Acinetobacter baumannii D1279779

94.493

100

0.945
  pilT Acinetobacter baumannii strain A118

94.493

100

0.945
  pilT Acinetobacter baylyi ADP1

86.919

99.71

0.867
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.048

97.391

0.672
  pilT Vibrio cholerae strain A1552

69.048

97.391

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

42.09

97.101

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

37.861

100

0.38