Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TPH_RS08305 Genome accession   NC_018870
Coordinates   1674897..1676000 (-) Length   367 a.a.
NCBI ID   WP_015050749.1    Uniprot ID   -
Organism   Thermacetogenium phaeum DSM 12270     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1669897..1681000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TPH_RS08280 (Tph_c16590) - 1670153..1670656 (-) 504 WP_015050744.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TPH_RS08285 (Tph_c16600) - 1670664..1671221 (-) 558 WP_015050745.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TPH_RS08290 (Tph_c16610) - 1671565..1672809 (+) 1245 WP_015050746.1 O-antigen ligase -
  TPH_RS08295 (Tph_c16620) - 1672882..1673313 (-) 432 WP_015050747.1 type IV pilin protein -
  TPH_RS08300 (Tph_c16630) - 1673672..1674895 (-) 1224 WP_015050748.1 type II secretion system F family protein -
  TPH_RS08305 (Tph_c16640) pilT 1674897..1676000 (-) 1104 WP_015050749.1 type IV pilus twitching motility protein PilT Machinery gene
  TPH_RS08310 (Tph_c16650) - 1676003..1677664 (-) 1662 WP_015050750.1 GspE/PulE family protein -
  TPH_RS08315 (Tph_c16660) - 1677977..1679008 (-) 1032 WP_015050751.1 type IV pilus twitching motility protein PilT -
  TPH_RS08320 (Tph_c16670) aroB 1679024..1680106 (-) 1083 WP_015050752.1 3-dehydroquinate synthase -
  TPH_RS08325 (Tph_c16680) - 1680087..1680647 (-) 561 WP_015050753.1 shikimate kinase -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40511.75 Da        Isoelectric Point: 6.4057

>NTDB_id=54110 TPH_RS08305 WP_015050749.1 1674897..1676000(-) (pilT) [Thermacetogenium phaeum DSM 12270]
MADINAILTQAAKLGASDVHITVGRPPVFRINGALFPLDDPQLRGRLDVIKPEEIGIFSPEDTDLLAREMMREDQYERYA
KKGELDFSYSLPGVTRVRANVFKQRNSTTLVMRLLSTTIPSFEELGLPPVVAYLSRRPHGLILVTGPTGSGKSTTLAAMI
DLINKEKRLHIITLEDPIEYLHRHKLGIVNQREIGEDTQSFATALRAALREDPDVILVGEMRDLETIATAITAAETGHLV
LATLHTSSAAETIDRIIDVFPSAQQQQVKVQLANTIEGVISQQLIPRKDVPGRALALEILVATPAIRNLIREGKTYQIPS
QIQTGGKYGMQTLDMSLRVLYRKGMIGKEEVLSRALEPESILGKGEE

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=54110 TPH_RS08305 WP_015050749.1 1674897..1676000(-) (pilT) [Thermacetogenium phaeum DSM 12270]
ATGGCAGACATTAACGCCATTCTCACCCAGGCTGCTAAGCTCGGTGCTTCCGATGTCCACATAACGGTCGGCAGGCCTCC
GGTCTTCAGGATCAACGGCGCCCTTTTCCCCCTTGATGACCCGCAGCTGAGAGGCCGGCTGGACGTAATCAAACCGGAGG
AGATCGGAATCTTTTCACCGGAGGATACAGATCTCCTGGCGCGGGAAATGATGAGGGAGGACCAGTACGAGCGATATGCG
AAAAAAGGGGAACTGGACTTCTCCTATTCGCTCCCCGGGGTTACCCGAGTGAGGGCGAACGTCTTCAAACAGAGGAACAG
CACCACCCTGGTGATGCGCCTGCTGAGCACGACGATCCCCTCTTTTGAGGAGCTGGGGCTCCCTCCGGTGGTTGCCTACC
TGTCCAGAAGGCCGCACGGCCTCATTTTGGTGACGGGCCCCACCGGCAGCGGTAAATCCACAACCCTGGCGGCGATGATC
GATTTAATTAACAAGGAGAAGAGGCTGCACATCATCACTCTGGAAGACCCCATCGAGTACCTCCACAGGCACAAGCTGGG
CATCGTCAACCAGCGGGAAATCGGCGAGGACACCCAATCCTTTGCCACGGCTCTGCGGGCGGCCTTGCGTGAGGACCCTG
ACGTCATCCTGGTGGGCGAGATGCGCGACCTGGAGACCATAGCCACAGCCATTACCGCTGCCGAAACCGGCCACCTTGTT
CTGGCAACCCTGCATACCTCCAGTGCCGCCGAAACCATCGACAGGATCATCGACGTCTTTCCCTCTGCCCAGCAGCAGCA
GGTGAAGGTGCAGCTGGCCAATACCATCGAGGGAGTAATTTCGCAGCAGCTGATACCCCGAAAAGACGTTCCGGGCCGGG
CGCTGGCCCTGGAGATCCTGGTGGCCACCCCCGCCATCAGAAATCTGATCCGTGAAGGGAAGACCTACCAGATACCTTCG
CAGATCCAGACGGGGGGGAAGTACGGTATGCAGACGCTGGACATGTCCCTGCGGGTGCTTTATCGGAAGGGAATGATCGG
CAAAGAAGAGGTTTTAAGCAGGGCGCTTGAGCCTGAGAGCATCCTGGGAAAAGGAGAGGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-286)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.482

97.82

0.523
  pilT Pseudomonas aeruginosa PAK

49.025

97.82

0.48
  pilT Legionella pneumophila strain Lp02

48.023

96.458

0.463
  pilT Legionella pneumophila strain ERS1305867

48.023

96.458

0.463
  pilT Acinetobacter baylyi ADP1

47.354

97.82

0.463
  pilT Pseudomonas stutzeri DSM 10701

47.354

97.82

0.463
  pilT Acinetobacter baumannii strain A118

47.222

98.093

0.463
  pilT Acinetobacter baumannii D1279779

47.222

98.093

0.463
  pilT Acinetobacter nosocomialis M2

47.222

98.093

0.463
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.727

95.913

0.458
  pilT Vibrio cholerae strain A1552

47.727

95.913

0.458
  pilT Neisseria meningitidis 8013

46.927

97.548

0.458
  pilT Neisseria gonorrhoeae MS11

46.648

97.548

0.455
  pilU Acinetobacter baylyi ADP1

39.167

98.093

0.384
  pilU Pseudomonas stutzeri DSM 10701

41.349

92.916

0.384
  pilU Vibrio cholerae strain A1552

39.118

92.643

0.362

Multiple sequence alignment