Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JW296_RS14980 Genome accession   NZ_CP070549
Coordinates   2596614..2597594 (-) Length   326 a.a.
NCBI ID   WP_119174540.1    Uniprot ID   A0AAD2PP53
Organism   Citrobacter freundii strain CF49969     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2591614..2602594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JW296_RS14950 (JW296_14945) - 2592390..2592887 (+) 498 WP_003838223.1 SprT family zinc-dependent metalloprotease -
  JW296_RS14955 (JW296_14950) endA 2592982..2593689 (+) 708 WP_016150969.1 deoxyribonuclease I -
  JW296_RS14960 (JW296_14955) rsmE 2593764..2594495 (+) 732 WP_003846360.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JW296_RS14965 (JW296_14960) gshB 2594515..2595462 (+) 948 WP_003027083.1 glutathione synthase -
  JW296_RS14970 (JW296_14965) - 2595638..2596201 (+) 564 WP_003027086.1 YqgE/AlgH family protein -
  JW296_RS14975 (JW296_14970) ruvX 2596201..2596617 (+) 417 WP_006686843.1 Holliday junction resolvase RuvX -
  JW296_RS14980 (JW296_14975) pilT 2596614..2597594 (-) 981 WP_119174540.1 type IV pilus twitching motility protein PilT Machinery gene
  JW296_RS14985 (JW296_14980) - 2597612..2598316 (+) 705 WP_003838215.1 YggS family pyridoxal phosphate-dependent enzyme -
  JW296_RS14990 (JW296_14985) - 2598335..2598901 (+) 567 WP_003825417.1 YggT family protein -
  JW296_RS14995 (JW296_14990) yggU 2598898..2599188 (+) 291 WP_003027101.1 DUF167 family protein YggU -
  JW296_RS15000 (JW296_14995) - 2599196..2599789 (+) 594 WP_119174541.1 XTP/dITP diphosphatase -
  JW296_RS15005 (JW296_15000) hemW 2599782..2600918 (+) 1137 WP_046670298.1 radical SAM family heme chaperone HemW -
  JW296_RS15010 (JW296_15005) ansB 2601016..2602062 (-) 1047 WP_003027108.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35942.98 Da        Isoelectric Point: 6.4255

>NTDB_id=540647 JW296_RS14980 WP_119174540.1 2596614..2597594(-) (pilT) [Citrobacter freundii strain CF49969]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRRRGKVESAPFTTPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRA
SAFVHQQGTSLALRLLPLDCPHLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHVLTLEDPIE
YRYTSRRCLIQQREVGAHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=540647 JW296_RS14980 WP_119174540.1 2596614..2597594(-) (pilT) [Citrobacter freundii strain CF49969]
ATGAATATGGAAGAAATAGTGACCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCCGCGCG
TTGGCGCAGGCGTGGAAAAGTCGAAAGCGCACCGTTTACCACGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGTCCATCAGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAATGGGTTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACGTTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGCACACTGCGCCTCTTTCGCCGCCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAAACCGGACATCTTGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTTCTTTCGCAAAAACTTGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTACTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGTTTGCAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.037

100

0.463
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Neisseria gonorrhoeae MS11

45.732

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Legionella pneumophila strain ERS1305867

45.342

98.773

0.448
  pilT Legionella pneumophila strain Lp02

45.342

98.773

0.448
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.009

97.239

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362