Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JW295_RS12895 Genome accession   NZ_CP070544
Coordinates   2021671..2022651 (-) Length   326 a.a.
NCBI ID   WP_058842385.1    Uniprot ID   -
Organism   Citrobacter freundii strain CF48846     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2016671..2027651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JW295_RS12865 (JW295_12870) - 2017447..2017944 (+) 498 WP_003838223.1 SprT family zinc-dependent metalloprotease -
  JW295_RS12870 (JW295_12875) endA 2018039..2018746 (+) 708 WP_058842381.1 deoxyribonuclease I -
  JW295_RS12875 (JW295_12880) rsmE 2018821..2019552 (+) 732 WP_058842382.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JW295_RS12880 (JW295_12885) gshB 2019572..2020519 (+) 948 WP_058842383.1 glutathione synthase -
  JW295_RS12885 (JW295_12890) - 2020695..2021258 (+) 564 WP_058842384.1 YqgE/AlgH family protein -
  JW295_RS12890 (JW295_12895) ruvX 2021258..2021674 (+) 417 WP_003027087.1 Holliday junction resolvase RuvX -
  JW295_RS12895 (JW295_12900) pilT 2021671..2022651 (-) 981 WP_058842385.1 type IV pilus twitching motility protein PilT Machinery gene
  JW295_RS12900 (JW295_12905) - 2022669..2023373 (+) 705 WP_049001602.1 YggS family pyridoxal phosphate-dependent enzyme -
  JW295_RS12905 (JW295_12910) - 2023392..2023958 (+) 567 WP_003825417.1 YggT family protein -
  JW295_RS12910 (JW295_12915) yggU 2023955..2024245 (+) 291 WP_003027101.1 DUF167 family protein YggU -
  JW295_RS12915 (JW295_12920) - 2024253..2024846 (+) 594 WP_003027104.1 XTP/dITP diphosphatase -
  JW295_RS12920 (JW295_12925) hemW 2024839..2025975 (+) 1137 WP_058842386.1 radical SAM family heme chaperone HemW -
  JW295_RS12925 (JW295_12930) ansB 2026091..2027137 (-) 1047 WP_044713806.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35874.05 Da        Isoelectric Point: 6.6317

>NTDB_id=540623 JW295_RS12895 WP_058842385.1 2021671..2022651(-) (pilT) [Citrobacter freundii strain CF48846]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRHGKVESAPFITPDVKNLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRA
SAFVHLQGTSLALRLLPLDCPHLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYASRRCLIQQREVGAHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=540623 JW295_RS12895 WP_058842385.1 2021671..2022651(-) (pilT) [Citrobacter freundii strain CF48846]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGTAATGCATGGCCCGCGCG
TTGGCGCAGACATGGAAAAGTCGAAAGCGCACCGTTTATCACGCCTGACGTAAAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGTCCATCTGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAATGGGTTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACGCCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGCACACTGCGCCTCTTTCGCCGCCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAAACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTTCTTTCGCAAAAACTTGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTACTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGTTTGCAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362