Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JTI59_RS06590 Genome accession   NZ_CP070511
Coordinates   1308387..1309430 (+) Length   347 a.a.
NCBI ID   WP_062753072.1    Uniprot ID   A0A6G9J076
Organism   Parageobacillus toebii strain NEB718     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1303387..1314430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JTI59_RS06575 (JTI59_06575) - 1304492..1305316 (+) 825 WP_062753078.1 PRC-barrel domain-containing protein -
  JTI59_RS06580 (JTI59_06580) - 1305337..1306698 (+) 1362 WP_205424533.1 VanW family protein -
  JTI59_RS06585 (JTI59_06585) - 1306707..1308374 (+) 1668 WP_062753074.1 GspE/PulE family protein -
  JTI59_RS06590 (JTI59_06590) pilT 1308387..1309430 (+) 1044 WP_062753072.1 type IV pilus twitching motility protein PilT Machinery gene
  JTI59_RS06595 (JTI59_06595) - 1309432..1310643 (+) 1212 WP_062753070.1 type II secretion system F family protein -
  JTI59_RS06600 (JTI59_06600) - 1310742..1311218 (+) 477 WP_205424534.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JTI59_RS06605 (JTI59_06605) - 1311229..1311975 (+) 747 WP_205424673.1 prepilin peptidase -
  JTI59_RS06610 (JTI59_06610) pilM 1311990..1312943 (+) 954 WP_205424535.1 type IV pilus biogenesis protein PilM -
  JTI59_RS06615 (JTI59_06615) - 1312933..1313529 (+) 597 WP_062753065.1 PilN domain-containing protein -
  JTI59_RS06620 (JTI59_06620) - 1313526..1314248 (+) 723 WP_205424536.1 pilus assembly protein PilO -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38411.63 Da        Isoelectric Point: 9.2938

>NTDB_id=540413 JTI59_RS06590 WP_062753072.1 1308387..1309430(+) (pilT) [Parageobacillus toebii strain NEB718]
MKQRLDALLRAAFELKASDVHLTVGVPPIFRINGELKRYGQDILRPEDTEGMAKAIIPSSLWPQFQEKGELDFSYGIPGV
SRFRINTYKQRSCVSLAIRIIPTKIPTIDELRLPEVLKQIAQKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMRKHIITL
EDPIEYLHKHGSCIIDQREVGFDTNNFANGLRAALRQDPDVILVGEMRDLETIHTAITAAETGHLVLGTLHTSSAPATID
RIIDVFPPAQQSQIRIQLASVLVAIISQRLFPTPQKTGRVAATEILINNTAVANLIRNEKVHQIINVMQTSRSLGMHTLA
MSIKELMQAGLILRDAAEPYLVQERGG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=540413 JTI59_RS06590 WP_062753072.1 1308387..1309430(+) (pilT) [Parageobacillus toebii strain NEB718]
ATGAAGCAAAGATTGGACGCTCTTTTACGTGCCGCGTTTGAACTAAAAGCTTCTGATGTTCATTTAACGGTCGGGGTGCC
GCCGATTTTTCGCATCAATGGCGAATTAAAAAGGTATGGCCAAGACATTTTGCGGCCGGAAGATACGGAAGGAATGGCAA
AAGCGATCATTCCGTCTAGCCTATGGCCGCAATTTCAAGAAAAAGGAGAATTGGATTTTTCGTACGGGATTCCAGGCGTT
TCCCGCTTCCGCATCAATACGTATAAACAGCGGTCATGTGTGTCGCTCGCTATCCGCATTATACCGACAAAAATTCCAAC
AATTGATGAACTGCGTCTTCCTGAAGTATTAAAACAGATCGCGCAAAAACCGCACGGGCTTGTGCTAGTGACAGGTCCGA
CGGGAAGCGGAAAATCAACGACGCTTGCGGCGATGATCGACTATATGAACAAAACGATGCGGAAACATATTATTACGCTT
GAGGATCCTATCGAATATTTGCACAAGCATGGCAGCTGCATTATTGACCAGCGTGAAGTTGGCTTTGACACGAACAATTT
TGCCAACGGTTTGCGCGCGGCGCTTCGTCAAGATCCCGATGTTATTTTAGTCGGGGAAATGCGCGATTTGGAAACGATTC
ATACCGCCATTACGGCGGCGGAAACGGGGCATCTTGTTCTTGGCACATTACATACATCGAGCGCGCCGGCGACAATCGAT
CGGATTATTGACGTGTTTCCGCCCGCCCAACAATCGCAAATTCGCATTCAGCTCGCGTCCGTTCTTGTCGCGATTATTTC
TCAGCGTCTTTTTCCAACACCGCAAAAAACAGGACGGGTCGCTGCCACGGAAATTTTAATTAATAACACGGCAGTCGCCA
ATTTAATTCGCAATGAAAAAGTCCATCAAATCATTAACGTTATGCAAACAAGCCGCTCCCTTGGCATGCATACGCTGGCG
ATGAGTATTAAAGAGTTAATGCAAGCCGGGCTGATCCTTCGCGATGCGGCTGAACCTTATCTTGTACAGGAGCGTGGCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G9J076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.744

94.524

0.499
  pilT Pseudomonas aeruginosa PAK

50.61

94.524

0.478
  pilT Pseudomonas stutzeri DSM 10701

50.456

94.813

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.849

95.389

0.476
  pilT Vibrio cholerae strain A1552

49.849

95.389

0.476
  pilT Acinetobacter baumannii strain A118

50.155

93.084

0.467
  pilT Acinetobacter nosocomialis M2

50.155

93.084

0.467
  pilT Acinetobacter baumannii D1279779

50.155

93.084

0.467
  pilT Acinetobacter baylyi ADP1

48.923

93.66

0.458
  pilT Neisseria meningitidis 8013

48.447

92.795

0.45
  pilT Neisseria gonorrhoeae MS11

48.447

92.795

0.45
  pilT Legionella pneumophila strain ERS1305867

46.061

95.101

0.438
  pilT Legionella pneumophila strain Lp02

46.061

95.101

0.438
  pilU Pseudomonas stutzeri DSM 10701

43.544

95.965

0.418
  pilU Acinetobacter baylyi ADP1

40.867

93.084

0.38
  pilU Vibrio cholerae strain A1552

40.367

94.236

0.38