Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JQN59_RS03980 Genome accession   NZ_CP069801
Coordinates   820066..821046 (+) Length   326 a.a.
NCBI ID   WP_149608187.1    Uniprot ID   -
Organism   Citrobacter sp. B72     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 815066..826046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JQN59_RS03950 (JQN59_03950) ansB 815581..816627 (+) 1047 WP_149608185.1 L-asparaginase 2 -
  JQN59_RS03955 (JQN59_03955) hemW 816742..817878 (-) 1137 WP_003825412.1 radical SAM family heme chaperone HemW -
  JQN59_RS03960 (JQN59_03960) - 817871..818464 (-) 594 WP_003825414.1 XTP/dITP diphosphatase -
  JQN59_RS03965 (JQN59_03965) yggU 818472..818762 (-) 291 WP_149608186.1 DUF167 family protein YggU -
  JQN59_RS03970 (JQN59_03970) - 818759..819325 (-) 567 WP_003825417.1 YggT family protein -
  JQN59_RS03975 (JQN59_03975) - 819344..820048 (-) 705 WP_103284733.1 YggS family pyridoxal phosphate-dependent enzyme -
  JQN59_RS03980 (JQN59_03980) pilT 820066..821046 (+) 981 WP_149608187.1 type IV pilus twitching motility protein PilT Machinery gene
  JQN59_RS03985 (JQN59_03985) ruvX 821043..821459 (-) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  JQN59_RS03990 (JQN59_03990) - 821459..822022 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  JQN59_RS03995 (JQN59_03995) gshB 822199..823146 (-) 948 WP_048215574.1 glutathione synthase -
  JQN59_RS04000 (JQN59_04000) rsmE 823166..823897 (-) 732 WP_149608188.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JQN59_RS04005 (JQN59_04005) endA 823972..824679 (-) 708 WP_008785767.1 deoxyribonuclease I -
  JQN59_RS04010 (JQN59_04010) - 824774..825271 (-) 498 WP_003825440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35990.15 Da        Isoelectric Point: 6.6559

>NTDB_id=536810 JQN59_RS03980 WP_149608187.1 820066..821046(+) (pilT) [Citrobacter sp. B72]
MNMEEIVALSVKHNVSDLHLCNAWRARWRIRGKVEIAPFTTPDVENLLMCWLSEQQQVQWQEQGQIDFAIALAGSRRLRA
SAFAHQQGTSLALRLLPLDCPRLDDLQTPDVIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQHVAGHILTLEDPIE
YRYTSQRCLIHQREVGVHCASFAAGLRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGAAQAIARMVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKLGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=536810 JQN59_RS03980 WP_149608187.1 820066..821046(+) (pilT) [Citrobacter sp. B72]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCGTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATCGCACCCTTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCAGGAACAGGGGCAGATTGATTTTGCTATTGCGCTGGCAGGTTCCCGGCGGCTGCGCGCC
AGTGCTTTTGCTCATCAACAGGGAACCTCGCTGGCGCTAAGATTGCTCCCGCTTGATTGCCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACTCTGGCGGCGATGGTAGAGTACCTGAATCAGCATGTTGCGGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCACCAACGGGAGGTGGGCGTACACTGCGCTTCTTTCGCCGCCGGTTTACG
CGGCGCGCTGCGTGAAGATCCCGACGTCATTTTGCTGGGCGAACTGCGCGACGCGGAAACCATTCGTCTGGCATTAACGG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGATGGTGGATACC
TTTCCCGCACAGGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCTGGGAGGACGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCAGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAACAGACAGGTATGCAGACATTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGTCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilU Acinetobacter baylyi ADP1

37.152

99.08

0.368