Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JN756_RS30150 Genome accession   NZ_CP069594
Coordinates   6602757..6603791 (-) Length   344 a.a.
NCBI ID   WP_046381759.1    Uniprot ID   A0A0R3B9J7
Organism   Pseudomonas sp. Y39-6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6597757..6608791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN756_RS30120 (JN756_29930) ruvX 6597908..6598345 (+) 438 WP_005792259.1 Holliday junction resolvase RuvX -
  JN756_RS30125 (JN756_29935) pyrR 6598430..6598936 (+) 507 WP_017845001.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  JN756_RS30130 (JN756_29940) - 6598961..6599965 (+) 1005 WP_017845002.1 aspartate carbamoyltransferase catalytic subunit -
  JN756_RS30135 (JN756_29945) - 6599962..6601233 (+) 1272 WP_057003953.1 dihydroorotase -
  JN756_RS30140 (JN756_29950) - 6601367..6601795 (-) 429 WP_017845004.1 NINE protein -
  JN756_RS30145 (JN756_29955) - 6602046..6602681 (+) 636 WP_046381760.1 C40 family peptidase -
  JN756_RS30150 (JN756_29960) pilT 6602757..6603791 (-) 1035 WP_046381759.1 type IV pilus twitching motility protein PilT Machinery gene
  JN756_RS30155 (JN756_29965) - 6603849..6604535 (+) 687 WP_017845007.1 YggS family pyridoxal phosphate-dependent enzyme -
  JN756_RS30160 (JN756_29970) proC 6604569..6605387 (+) 819 WP_017845008.1 pyrroline-5-carboxylate reductase -
  JN756_RS30165 (JN756_29975) - 6605427..6606014 (+) 588 WP_017845009.1 YggT family protein -
  JN756_RS30170 (JN756_29980) - 6606119..6607258 (+) 1140 WP_046381757.1 homoserine O-acetyltransferase -
  JN756_RS30175 (JN756_29985) metW 6607266..6607886 (+) 621 WP_017845011.1 methionine biosynthesis protein MetW -
  JN756_RS30180 (JN756_29990) - 6607908..6608342 (+) 435 WP_201021623.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37488.25 Da        Isoelectric Point: 8.0512

>NTDB_id=535866 JN756_RS30150 WP_046381759.1 6602757..6603791(-) (pilT) [Pseudomonas sp. Y39-6]
MDITELLTHSVRRGASDLHLSAGLAPMLRMDGEVWSLDGPVLSPTHVADLLSPLLNQYQQKDFETSLETDFAFELPGVGR
FRVNVFQQARGMGAVFRNIPAQVQSLENLGLGAVFQRIAQLPRGLVLVTGPTGSGKSTTLAALIDHLNQHRRQHILTLED
PIEFIHQPCRALINQRQVHRDTHSFSAALRSALRQDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTASAAKTVDRL
VDVFPAGEKPMVRSMLSESLQAVVSQVLVKKIGGGRVAAHEIMLCTPAIRNLIREDKVAQIVSAIQTGGASGMQTLDMRL
KALVTQGVISREAAREKARVPTDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=535866 JN756_RS30150 WP_046381759.1 6602757..6603791(-) (pilT) [Pseudomonas sp. Y39-6]
ATGGATATCACTGAATTACTCACGCACAGCGTGCGCCGGGGCGCCTCCGACCTGCACCTGTCGGCCGGCCTCGCACCGAT
GCTGCGGATGGATGGCGAGGTCTGGTCCCTGGATGGGCCAGTGCTGTCACCCACGCACGTGGCGGATTTGCTCAGCCCTT
TGCTCAACCAGTACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTCGCCTTCGAACTGCCCGGTGTCGGGCGA
TTCCGGGTGAATGTGTTCCAGCAGGCGCGTGGCATGGGCGCGGTGTTTCGCAACATCCCCGCCCAGGTCCAGAGCCTGGA
AAACCTCGGGTTGGGCGCGGTGTTCCAACGTATCGCCCAACTGCCGCGCGGCCTGGTGCTGGTGACCGGGCCCACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCGCTGATCGATCACCTCAACCAGCACCGGCGCCAGCACATCCTCACCTTGGAAGAC
CCCATCGAGTTCATCCACCAGCCCTGCCGGGCCCTGATCAACCAGCGCCAGGTGCACCGCGACACCCACAGTTTTTCGGC
GGCCTTGCGTTCGGCACTGCGGCAAGACCCGGATGTGATCCTGGTGGGTGAACTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCTGAGACCGGGCATCTGGTCTTCGGCACCCTGCACACCGCGTCGGCGGCCAAGACCGTGGATCGCCTG
GTGGACGTGTTTCCGGCGGGAGAAAAGCCCATGGTCCGCTCGATGCTCTCGGAGTCGCTGCAGGCGGTGGTGTCCCAGGT
GTTGGTCAAGAAGATCGGCGGCGGGCGCGTGGCGGCCCATGAAATCATGCTGTGTACGCCCGCCATTCGCAATTTGATCC
GCGAAGACAAGGTGGCGCAGATCGTATCGGCGATTCAGACCGGTGGCGCATCGGGGATGCAGACGTTGGATATGCGCTTG
AAGGCCTTGGTCACGCAGGGCGTGATCAGCCGGGAGGCGGCGCGGGAGAAGGCGAGGGTGCCGACAGACATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0R3B9J7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

70.64

100

0.706
  pilT Pseudomonas stutzeri DSM 10701

68.605

100

0.686
  pilT Acinetobacter nosocomialis M2

68.035

99.128

0.674
  pilT Acinetobacter baumannii D1279779

68.035

99.128

0.674
  pilT Acinetobacter baumannii strain A118

68.035

99.128

0.674
  pilT Acinetobacter baylyi ADP1

67.155

99.128

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.095

97.965

0.628
  pilT Vibrio cholerae strain A1552

64.095

97.965

0.628
  pilT Legionella pneumophila strain Lp02

63.609

98.256

0.625
  pilT Legionella pneumophila strain ERS1305867

63.609

98.256

0.625
  pilT Neisseria gonorrhoeae MS11

61.471

98.837

0.608
  pilT Neisseria meningitidis 8013

61.471

98.837

0.608
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.497
  pilU Pseudomonas stutzeri DSM 10701

39.71

100

0.398
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395