Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TOL2_RS03110 Genome accession   NC_018645
Coordinates   684661..685743 (+) Length   360 a.a.
NCBI ID   WP_014956093.1    Uniprot ID   K0NIC8
Organism   Desulfobacula toluolica Tol2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 679661..690743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOL2_RS03090 (TOL2_05650) thiS 681064..681261 (-) 198 WP_014956089.1 sulfur carrier protein ThiS -
  TOL2_RS03095 (TOL2_05660) - 681258..683399 (-) 2142 WP_014956090.1 aldehyde ferredoxin oxidoreductase family protein -
  TOL2_RS03100 (TOL2_05670) - 683806..684105 (+) 300 WP_014956091.1 YggT family protein -
  TOL2_RS03105 (TOL2_05680) - 684124..684624 (+) 501 WP_014956092.1 DivIVA domain-containing protein -
  TOL2_RS03110 (TOL2_05690) pilT 684661..685743 (+) 1083 WP_014956093.1 type IV pilus twitching motility protein PilT Machinery gene
  TOL2_RS03115 (TOL2_05700) - 685792..686952 (+) 1161 WP_014956094.1 type IV pilus twitching motility protein PilT -
  TOL2_RS03120 (TOL2_05710) - 686960..687544 (+) 585 WP_014956095.1 zinc-ribbon domain-containing protein -
  TOL2_RS03125 (TOL2_05720) - 687656..688468 (+) 813 WP_232508043.1 hypothetical protein -
  TOL2_RS03130 (TOL2_05730) - 688465..690465 (+) 2001 WP_014956097.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39852.61 Da        Isoelectric Point: 6.8357

>NTDB_id=53474 TOL2_RS03110 WP_014956093.1 684661..685743(+) (pilT) [Desulfobacula toluolica Tol2]
MAEIDAFFKLMHEQGASDLHLASGQQPALRLHGDIERIKYDTLTSDKLRGLLYEITSQEKIKVFEETGDVDFGYEIPGLA
RYRANYFMQKSGISAVFREIPSEILTAEQLGLPPVISKLADLPRGLVLVTGPTGSGKSTTLAAIVDQANRNRKDHIITVE
DPIEFVHRSQNCIVNHREVGMHTQTFSSALRGALREDPDIILVGELRDLETISLAIEAASTGHLVFGTLHTSSAAKTVDR
IIEVFPSTEQAQIRSTLSDGLRAIVAQTLFKRVDKKGRCAALEILVATPAVRNLIREAKTHQIPSMIQTGKQYGMQLLDD
AIMQLYKKGWISPDEAYGKANNKSLFRPFLKNPPSDFTEA

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=53474 TOL2_RS03110 WP_014956093.1 684661..685743(+) (pilT) [Desulfobacula toluolica Tol2]
ATGGCTGAAATTGATGCTTTTTTTAAGCTGATGCATGAGCAGGGTGCTTCCGATCTCCATCTGGCCTCGGGCCAGCAACC
GGCATTAAGACTTCATGGCGATATTGAACGTATAAAATATGATACATTGACCAGTGATAAACTAAGGGGCCTTTTATATG
AAATAACCTCCCAGGAGAAGATTAAGGTTTTTGAAGAAACCGGGGATGTGGATTTTGGTTATGAAATCCCTGGGCTTGCC
AGGTACAGGGCAAACTATTTTATGCAAAAAAGCGGTATCTCAGCAGTTTTCCGCGAGATCCCGTCTGAAATACTGACTGC
AGAACAGCTGGGGCTTCCACCGGTTATTTCAAAACTGGCTGATCTTCCAAGGGGTCTTGTTCTGGTTACAGGACCTACCG
GGTCAGGTAAGTCAACTACCCTTGCCGCTATTGTTGATCAGGCCAATAGAAACAGAAAAGATCATATCATTACTGTGGAA
GACCCCATTGAGTTTGTACACCGAAGCCAGAACTGCATTGTGAACCACAGGGAAGTGGGAATGCACACCCAGACATTTTC
ATCAGCCTTGAGAGGGGCCCTTCGTGAAGACCCGGATATTATTCTCGTGGGGGAATTAAGGGATCTTGAAACCATTTCCC
TTGCCATTGAAGCGGCTTCCACAGGCCATCTGGTGTTTGGAACCCTGCATACCTCCAGTGCTGCTAAAACCGTTGACAGG
ATTATCGAAGTGTTCCCCTCCACAGAACAGGCCCAGATACGCTCTACCTTGTCTGACGGTCTTCGCGCCATTGTGGCCCA
GACATTGTTTAAGAGAGTTGATAAAAAAGGCAGGTGTGCAGCTTTGGAAATTTTAGTGGCAACACCTGCCGTGCGAAATC
TTATCCGTGAAGCCAAAACTCACCAGATTCCATCCATGATTCAGACCGGCAAGCAATACGGGATGCAATTGCTGGATGAT
GCCATCATGCAATTGTATAAAAAAGGATGGATCAGCCCTGATGAGGCATATGGAAAAGCTAATAATAAATCCTTGTTCAG
GCCTTTTCTGAAGAACCCGCCGTCTGATTTTACGGAAGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K0NIC8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

55.523

95.556

0.531
  pilT Legionella pneumophila strain Lp02

56.798

91.944

0.522
  pilT Legionella pneumophila strain ERS1305867

56.798

91.944

0.522
  pilT Pseudomonas aeruginosa PAK

55.325

93.889

0.519
  pilT Acinetobacter nosocomialis M2

53.892

92.778

0.5
  pilT Acinetobacter baumannii D1279779

53.892

92.778

0.5
  pilT Acinetobacter baumannii strain A118

53.892

92.778

0.5
  pilT Acinetobacter baylyi ADP1

54.079

91.944

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.517

90.833

0.486
  pilT Vibrio cholerae strain A1552

53.517

90.833

0.486
  pilT Neisseria meningitidis 8013

51.988

90.833

0.472
  pilT Neisseria gonorrhoeae MS11

51.682

90.833

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.512

95.556

0.444
  pilU Pseudomonas stutzeri DSM 10701

39.542

96.944

0.383
  pilU Acinetobacter baylyi ADP1

36.364

100

0.367
  pilU Vibrio cholerae strain A1552

36.723

98.333

0.361

Multiple sequence alignment