Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JQN63_RS29105 Genome accession   NZ_CP069318
Coordinates   6375506..6376549 (+) Length   347 a.a.
NCBI ID   WP_203400072.1    Uniprot ID   -
Organism   Delftia lacustris strain R54     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6370506..6381549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JQN63_RS29085 (JQN63_29090) - 6370896..6372491 (-) 1596 WP_203400068.1 AMP-binding protein -
  JQN63_RS29090 (JQN63_29095) - 6372651..6373373 (+) 723 WP_203400069.1 TetR/AcrR family transcriptional regulator -
  JQN63_RS29095 (JQN63_29100) - 6373400..6374683 (+) 1284 WP_203400070.1 branched-chain amino acid ABC transporter substrate-binding protein -
  JQN63_RS29100 (JQN63_29105) - 6374743..6375468 (-) 726 WP_203400071.1 YggS family pyridoxal phosphate-dependent enzyme -
  JQN63_RS29105 (JQN63_29110) pilT 6375506..6376549 (+) 1044 WP_203400072.1 type IV pilus twitching motility protein PilT Machinery gene
  JQN63_RS29110 (JQN63_29115) - 6376581..6377213 (+) 633 WP_016450724.1 cyclic nucleotide-binding domain-containing protein -
  JQN63_RS29115 (JQN63_29120) pilU 6377343..6378479 (+) 1137 WP_016450725.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JQN63_RS29120 (JQN63_29125) - 6378580..6379491 (+) 912 WP_016450726.1 NAD(P)-dependent oxidoreductase -
  JQN63_RS29125 (JQN63_29130) - 6379690..6380421 (-) 732 WP_016451991.1 BON domain-containing protein -
  JQN63_RS29130 (JQN63_29135) - 6380418..6381026 (-) 609 WP_016451992.1 SIS domain-containing protein -
  JQN63_RS29135 (JQN63_29140) - 6381100..6381516 (-) 417 WP_017406037.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38309.02 Da        Isoelectric Point: 6.8352

>NTDB_id=533498 JQN63_RS29105 WP_203400072.1 6375506..6376549(+) (pilT) [Delftia lacustris strain R54]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKTVHAMVYDIMSDSQRKAYEEFLEVDFSFEIEGLAR
FRVNAFNQYRGAAAVFRTIPSKILTLEQLNAPRIFADLALKPRGLVLVTGPTGSGKSTTLAGMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMSQSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRIAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSQNVGMQTLDQN
LTDLVRRNLISPAEARAKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=533498 JQN63_RS29105 WP_203400072.1 6375506..6376549(+) (pilT) [Delftia lacustris strain R54]
GTGGACATCACCCAATTGCTGGCTTTCAGCGTCAAGAACAAGGCCTCCGACCTGCACCTGTCCGCGGGGCTGCCGCCGAT
GATCCGCGTGCACGGCGATGTGCGCCGCATCAATGTCGATCCGCTGGACCACAAGACGGTCCACGCCATGGTCTACGACA
TCATGAGCGACTCCCAGCGCAAGGCCTACGAGGAGTTCCTGGAGGTGGACTTCTCGTTCGAGATCGAGGGCCTGGCGCGC
TTTCGTGTCAACGCCTTCAACCAGTACCGGGGCGCGGCCGCCGTCTTCCGTACCATTCCCAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCGCCGCGCATCTTCGCCGACCTGGCCCTGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACAGGCT
CGGGCAAGTCCACCACGCTGGCCGGCATGGTCAACCACCTCAACGAGACCGAGTACGGCCACATCCTGACGGTGGAGGAC
CCCATCGAATTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGTCGCAGTCGTTCTCGGC
CGCCCTGCGCTCGGCGCTGCGCGAGGACCCGGACGCCATCCTGGTGGGCGAAATGCGTGACCTCGAAACCATCCGCCTGG
CCATGACCGCGGCCGAGACGGGCCACCTGGTCTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGCATC
ATCGACGTCTTCCCGGCCGAGGAAAAGGAGATGGTGCGCGCCATGCTTTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCATCGCGGCCCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCCCAGATGTACTCCACCATCCAGACGAGTCAGAATGTGGGCATGCAGACGCTGGACCAGAAC
CTGACCGATCTGGTGCGGCGCAACCTGATCAGCCCGGCCGAAGCGCGCGCCAAGGCCAAGATACCGGACAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.362

99.424

0.749
  pilT Acinetobacter nosocomialis M2

75.072

99.424

0.746
  pilT Acinetobacter baumannii D1279779

75.072

99.424

0.746
  pilT Acinetobacter baumannii strain A118

75.072

99.424

0.746
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Acinetobacter baylyi ADP1

72.754

99.424

0.723
  pilT Legionella pneumophila strain Lp02

72.861

97.695

0.712
  pilT Legionella pneumophila strain ERS1305867

72.861

97.695

0.712
  pilT Neisseria meningitidis 8013

67.941

97.983

0.666
  pilT Neisseria gonorrhoeae MS11

67.647

97.983

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.982

98.271

0.648
  pilT Vibrio cholerae strain A1552

65.982

98.271

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

42.687

96.542

0.412
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395